FGFBP1 (NM_005130) Human Tagged ORF Clone

CAT#: RC200819

FGFBP1 (Myc-DDK-tagged)-Human fibroblast growth factor binding protein 1 (FGFBP1)

ORF Plasmid: DDK tGFP



  "NM_005130" in other vectors (6)

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CNY 2,400.00

CNY 3,705.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


FGFBP1 mouse monoclonal antibody, clone OTI2B6 (formerly 2B6)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms FGF-BP; FGF-BP1; FGFBP; FGFBP-1; HBP17
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200819 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGATCTGTAGCCTCACCCTGCTCTCCTTCCTCCTACTGGCTGCTCAGGTGCTCCTGGTGGAGGGGA
AAAAAAAAGTGAAGAATGGACTTCACAGCAAAGTGGTCTCAGAACAAAAGGACACTCTGGGCAACACCCA
GATTAAGCAGAAAAGCAGGCCCGGGAACAAAGGCAAGTTTGTCACCAAAGACCAAGCCAACTGCAGATGG
GCTGCTACTGAGCAGGAGGAGGGCATCTCTCTCAAGGTTGAGTGCACTCAATTGGACCATGAATTTTCCT
GTGTCTTTGCTGGCAATCCAACCTCATGCCTAAAGCTCAAGGATGAGAGAGTCTATTGGAAACAAGTTGC
CCGGAATCTGCGCTCACAGAAAGACATCTGTAGATATTCCAAGACAGCTGTGAAAACCAGAGTGTGCAGA
AAGGATTTTCCAGAATCCAGTCTTAAGCTAGTCAGCTCCACTCTATTTGGGAACACAAAGCCCAGGAAGG
AGAAAACAGAGATGTCCCCCAGGGAGCACATCAAGGGCAAAGAGACCACCCCCTCTAGCCTAGCAGTGAC
CCAGACCATGGCCACCAAAGCTCCCGAGTGTGTGGAGGACCCAGATATGGCAAACCAGAGGAAGACTGCC
CTGGAGTTCTGTGGAGAGACTTGGAGCTCTCTCTGCACATTCTTCCTCAGCATAGTGCAGGACACGTCAT
GC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200819 protein sequence
Red=Cloning site Green=Tags(s)

MKICSLTLLSFLLLAAQVLLVEGKKKVKNGLHSKVVSEQKDTLGNTQIKQKSRPGNKGKFVTKDQANCRW
AATEQEEGISLKVECTQLDHEFSCVFAGNPTSCLKLKDERVYWKQVARNLRSQKDICRYSKTAVKTRVCR
KDFPESSLKLVSSTLFGNTKPRKEKTEMSPREHIKGKETTPSSLAVTQTMATKAPECVEDPDMANQRKTA
LEFCGETWSSLCTFFLSIVQDTSC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005130
ORF Size 702 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005130.2
RefSeq Size 1369 bp
RefSeq ORF 705 bp
Locus ID 9982
UniProt ID Q14512
Protein Families Secreted Protein
MW 26.3 kDa
Gene Summary This gene encodes a secreted fibroblast growth factor carrier protein. The encoded protein plays a critical role in cell proliferation, differentiation and migration by binding to fibroblast growth factors and potentiating their biological effects on target cells. The encoded protein may also play a role in tumor growth as an angiogenic switch molecule, and expression of this gene has been associated with several types of cancer including pancreatic and colorectal adenocarcinoma. A pseudogene of this gene is also located on the short arm of chromosome 4. [provided by RefSeq, Nov 2011]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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