FIBP (NM_004214) Human Tagged ORF Clone

CAT#: RC200088

FIBP (Myc-DDK-tagged)-Human fibroblast growth factor (acidic) intracellular binding protein (FIBP), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_004214" in other vectors (4)

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CNY 3,656.00

CNY 5,320.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


FIBP Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms FGFIBP; FIBP-1; TROFAS
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200088 representing NM_004214
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCAGTGAGCTGGACATCTTCGTGGGGAACACGACCCTTATCGACGAGGACGTGTATCGCCTCTGGC
TCGATGGTTACTCGGTGACCGACGCGGTGGCCCTGCGGGTGCGCTCGGGAATCCTGGAGCAGACTGGCGC
CACGGCAGCGGTGCTGCAGAGCGACACCATGGACCATTACCGCACCTTCCACATGCTCGAGCGGCTGCTG
CATGCGCCGCCCAAGCTACTGCACCAGCTCATCTTCCAGATTCCGCCCTCCCGGCAGGCACTACTCATCG
AGAGGTACTATGCCTTTGATGAGGCCTTTGTTCGGGAGGTGCTGGGCAAGAAGCTGTCCAAAGGCACCAA
GAAAGACCTGGATGACATCAGCACCAAAACAGGCATCACCCTCAAGAGCTGCCGGAGACAGTTTGACAAC
TTTAAACGGGTCTTCAAGGTGGTAGAGGAAATGCGGGGCTCCCTGGTGGACAATATTCAGCAACACTTCC
TCCTCTCTGACCGGTTGGCCAGGGACTATGCAGCCATCGTCTTCTTTGCTAACAACCGCTTTGAGACAGG
GAAGAAAAAACTGCAGTATCTGAGCTTCGGTGACTTTGCCTTCTGCGCTGAGCTCATGATCCAAAACTGG
ACCCTTGGAGCCGTCGACTCACAGATGGATGACATGGACATGGACTTAGACAAGGAATTTCTCCAGGACT
TGAAGGAGCTCAAGGTGCTAGTGGCTGACAAGGACCTTCTGGACCTGCACAAGAGCCTGGTGTGCACTGC
TCTCCGGGGAAAGCTGGGCGTCTTCTCTGAGATGGAAGCCAACTTCAAGAACCTGTCCCGGGGGCTGGTG
AACGTGGCCGCCAAGCTGACCCACAATAAAGATGTCAGAGACCTGTTTGTGGACCTCGTGGAGAAGTTTG
TGGAACCCTGCCGCTCCGACCACTGGCCACTCAGCGACGTGCGGTTCTTCCTGAATCAGTATTCAGCGTC
TGTCCACTCCCTCGATGGCTTCCGACACCAGGCCCTCTGGGACCGCTACATGGGCACCCTCCGCGGCTGC
CTCCTGCGCCTGTATCATGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200088 representing NM_004214
Red=Cloning site Green=Tags(s)

MTSELDIFVGNTTLIDEDVYRLWLDGYSVTDAVALRVRSGILEQTGATAAVLQSDTMDHYRTFHMLERLL
HAPPKLLHQLIFQIPPSRQALLIERYYAFDEAFVREVLGKKLSKGTKKDLDDISTKTGITLKSCRRQFDN
FKRVFKVVEEMRGSLVDNIQQHFLLSDRLARDYAAIVFFANNRFETGKKKLQYLSFGDFAFCAELMIQNW
TLGAVDSQMDDMDMDLDKEFLQDLKELKVLVADKDLLDLHKSLVCTALRGKLGVFSEMEANFKNLSRGLV
NVAAKLTHNKDVRDLFVDLVEKFVEPCRSDHWPLSDVRFFLNQYSASVHSLDGFRHQALWDRYMGTLRGC
LLRLYHD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004214
ORF Size 1071 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004214.5
RefSeq Size 1429 bp
RefSeq ORF 1074 bp
Locus ID 9158
UniProt ID O43427
MW 41 kDa
Gene Summary Acidic fibroblast growth factor is mitogenic for a variety of different cell types and acts by stimulating mitogenesis or inducing morphological changes and differentiation. The FIBP protein is an intracellular protein that binds selectively to acidic fibroblast growth factor (aFGF). It is postulated that FIBP may be involved in the mitogenic action of aFGF. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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