Adcy8 (NM_001291903) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AC8; AW060868
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231694 representing NM_001291903 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTCTCGGATGTGCACTGCCTTAGTGGCAGCGAGGAACTCTACACCATCCAACCGACGCCCCCGG CCGGCGACGACGGGAGCGGCTCTCGGCCGCAGCGGCTGCTGTGGCAGACGGCGGTGCGGCACATCACGGA GCAGCGCTTCATCCACGGGCACCGAGGCGGCGGCGGCGGCGGGGTCTCCCGCAAAGCCTCGAACCCTGCG GGCAGTGGACCCAATCACCACGCGCCGCAGCTGTCTAGCGACTCGGTGCTGCCTCTCTATTCTCTGGGCC CCGGAGAGCGAGCGCACAACACCGGTGGCACCAAAGTCTTTCCGGAACGCAGCGGGAGCGGCAGTGCCAG TGGCAGCGGGGGCGGGGGCGACTTGGGCTTCCTACACCTTGACTGTGCCCCAAGTAACTCGGATTTCTTC CTCAATGGAGGATACAGCTACCGAGGGGTCATTTTTCCAACCCTACGCAACTCCTTCAAGTCTCGGGATC TGGAGCGCCTCTACCAACGCTATTTCCTGGGCCAGAGGCGCAAATCGGAGGTAGTGATGAACGTGTTGGA TGTACTAACCAAACTCACCCTTTTAGTCCTGCACTTGAGCCTGGCCTCGGCTCCAATGGACCCTCTCAAG GGCATCCTGCTAGGCTTTTTCACTGGCATCGAGGTGGTGATCTGCGCCCTCGTGGTGGTCAGGAAGGACA ACACCTCCCACACTTACCTGCAATACAGCGGCGTGGTCACTTGGGTGGCTATGACCACCCAGATTCTGGC AGCAGGCCTGGGCTATGGGCTTCTGGGCGACGGCATAGGCTACGTGCTTTTTACACTCTTCGCCACCTAC AGCATGCTTCCCCTGCCTCTCACCTGGGCCATCTTGGCCGGCCTGGGCACATCTTTGCTGCAAGTCACCC TTCAAGTACTCATACCCAGGCTAGCGGTCTTTTCCATCAACCAGGTCCTGGCCCAGGTGGTGCTATTCAT GTGTATGAATACAGCAGGAATCTTCATCAGTTACCTTTCAGACCGTGCCCAGCGCCAGGCCTTCCTGGAG ACCCGGAGGTGTGTGGAGGCCAGGCTCCGCTTGGAGACAGAGAACCAAAGACAGGAGCGGCTTGTGCTGT CTGTGCTCCCCAGGTTTGTCGTCCTGGAAATGATCAACGACATGACCAATGTGGAGGATGAGCACCTGCA GCATCAGTTCCACCGCATCTACATCCATCGCTATGAGAACGTCAGTATTCTTTTTGCAGATGTCAAAGGA TTTACCAACCTCTCTACGACCTTGTCTGCTCAGGAGCTGGTCAGGATGCTCAACGAACTCTTTGCCAGAT TCGACCGGCTGGCCCATGAGCATCACTGTCTTCGCATTAAAATCCTGGGGGACTGCTACTACTGTGTGTC TGGACTGCCTGAGCCCCGCCGGGACCACGCTCATTGCTGTGTTGAAATGGGCCTCAGCATGATCAAAACT ATCAGGTTTGTGAGATCCAGAACGAAGCACGATGTTGACATGCGAATTGGAATCCATTCAGGTTCTGTGC TATGTGGTGTGTTGGGCCTGAGGAAATGGCAGTTTGATGTCTGGTCTTGGGATGTGGACATAGCAAACAA GCTTGAGTCTGGAGGAATCCCTGGGAGGATTCACATTTCTAAAGCCACCCTGGATTGCCTCAATGGTGAC TATAACGTGGAAGAGGGTCACGGGAAAGAGAGGAACGAATTCTTGAGGAAGCATAACATAGAGACCTATT TGATTAAGCAGCCTGAGGAGAGTTTGCTCTGCTTGCCTGAAGACATCGTTAAGGAGTCGGTGAGCTGCTC TGACAGGAGAAACAGTGGAGCAACATTCACAGAAGGATCGTGGAGCCCAGAGCTGCCTTTCGACAACATC GTGGGCAAACAGAATACTCTGGCTGCCCTAACAAGAAATTCAATAAATCTGCTTCCAAACCATCTCGCAC AAGCTTTGCATGTCCAGTCTGGGCCTGAGGAAATTAACAAGAGAATAGAGCATACCATCGACTTGCGGAG TGGCGATAAGTTGAGAAGAGAGCATATCAAGCCATTCTCACTGATGTTTAAAGACTCCAGCCTGGAGCAC AAGTATTCTCAAATGAGGGATGAAGTATTCAAGTCAAACTTGGTCTGTGCATTTATCGTTCTTCTGTTTA TCACTGCAATTCAAAGTTTGCTTCCTTCTTCAAGGCTGATGCCTATGACTATCCAGTTCTCCATCCTGAT CATGCTGCACTCTGCCCTGGTCCTCATCACCACAGCAGAAGACTATAAGTGTCTGCCCCTCATCCTCCGA AAAACCTGCTGTTGGATTAATGAGACCTATCTGGCCCGCAACGTCATCATCTTCGCTTCCATCTTAATTA ACTTCCTGGGAGCTGTCCTAAATATTTACTTTGTCTTCACTGGGGTGTTGGCCATGGTGACCTGTGCTGT GTTTCTACGGCTTAACTCTGTCCTGAAGCTGGCAGTGCTACTCATCATGATCGCCATCTACGCCCTGCTC ACAGAGACCATCTACGCAGGTCTCTTTCTGAGTTATGACAACCTGAACCACAGTGGAGAAGATTTCCTGG GGACCAAGGAAGCATCACTGCTGCTGATGGCCATGTTCCTTCTTGCTGTGTTCTACCATGGACAACAGCT GGAGTACACAGCCCGCCTGGATTTCCTGTGGCGCGTACAGGCCAAAGAGGAGATCAACGAGATGAAGGAA CTGAGGGAACACAACGAGAACATGCTGCGCAATATCTTACCCAGCCATGTGGCCCGCCACTTCCTGGAGA AAGACAGAGACAATGAGGAGCTGTATTCTCAATCCTACGATGCTGTTGGAGTGATGTTCGCCTCCATCCC TGGGTTCGCAGACTTCTACTCTCAGACAGAAATGAACAACCAGGGAGTAGAATGTCTGCGCTTGCTGAAT GAGATCATTGCTGACTTTGATGAGCTGCTCGGGGAGGACCGGTTTCAGGACATTGAGAAGATTAAGACCA TTGGTAGTACATACATGGCTGTCTCAGGACTGTCCCCAGAGAAACAGCAATGTGAAGATAAATGGGGACA TTTGTGTGCCCTGGCTGACTTCTCTCTTGCTCTGACTGAAAGCATACAAGAGATCAACAAGCATTCATTC AACAATTTTGAACTCCGAATCGGCATCAGCCATGGCTCAGTGGTGGCAGGTGTAATTGGAGCTAAGAAAC CACAGTATGACATTTGGGGGAAAACTGTGAACTTGGCAAGCCGAATGGACAGCACGGGAGTAAGTGGCCG GATCCAAGTTCCTGAGGAGACCTACCTTATCCTGAAGGACCAGGGCTTTGCCTTTGACTACCGTGGGGAG ATATATGTGAAGGGCATCAGTGAACAAGAAGGGAAAATCAAAACATACTTTCTCCTGGGACGAGTCCAAC CCAACCCATTCATCTTACCCCCAAGGAGACTTCCCGGGCAATACTCTCTGGCTGCGGTTGTCCTTGGCCT TGTCCAGTCTCTCAACAGGCAAAGGCAGAAGCAACTTCTCAACGAGAACAGCAATTCGGGCATCATCAAG AGCCATTACAACCGGCGGACTTTGCTAACGCCAAGTGGGCCAGAGCCTGGAGCACAAGCTGAAGGCACTG ACAAATCCGATTTGCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231694 representing NM_001291903 Red=Cloning site Green=Tags(s) MELSDVHCLSGSEELYTIQPTPPAGDDGSGSRPQRLLWQTAVRHITEQRFIHGHRGGGGGGVSRKASNPA GSGPNHHAPQLSSDSVLPLYSLGPGERAHNTGGTKVFPERSGSGSASGSGGGGDLGFLHLDCAPSNSDFF LNGGYSYRGVIFPTLRNSFKSRDLERLYQRYFLGQRRKSEVVMNVLDVLTKLTLLVLHLSLASAPMDPLK GILLGFFTGIEVVICALVVVRKDNTSHTYLQYSGVVTWVAMTTQILAAGLGYGLLGDGIGYVLFTLFATY SMLPLPLTWAILAGLGTSLLQVTLQVLIPRLAVFSINQVLAQVVLFMCMNTAGIFISYLSDRAQRQAFLE TRRCVEARLRLETENQRQERLVLSVLPRFVVLEMINDMTNVEDEHLQHQFHRIYIHRYENVSILFADVKG FTNLSTTLSAQELVRMLNELFARFDRLAHEHHCLRIKILGDCYYCVSGLPEPRRDHAHCCVEMGLSMIKT IRFVRSRTKHDVDMRIGIHSGSVLCGVLGLRKWQFDVWSWDVDIANKLESGGIPGRIHISKATLDCLNGD YNVEEGHGKERNEFLRKHNIETYLIKQPEESLLCLPEDIVKESVSCSDRRNSGATFTEGSWSPELPFDNI VGKQNTLAALTRNSINLLPNHLAQALHVQSGPEEINKRIEHTIDLRSGDKLRREHIKPFSLMFKDSSLEH KYSQMRDEVFKSNLVCAFIVLLFITAIQSLLPSSRLMPMTIQFSILIMLHSALVLITTAEDYKCLPLILR KTCCWINETYLARNVIIFASILINFLGAVLNIYFVFTGVLAMVTCAVFLRLNSVLKLAVLLIMIAIYALL TETIYAGLFLSYDNLNHSGEDFLGTKEASLLLMAMFLLAVFYHGQQLEYTARLDFLWRVQAKEEINEMKE LREHNENMLRNILPSHVARHFLEKDRDNEELYSQSYDAVGVMFASIPGFADFYSQTEMNNQGVECLRLLN EIIADFDELLGEDRFQDIEKIKTIGSTYMAVSGLSPEKQQCEDKWGHLCALADFSLALTESIQEINKHSF NNFELRIGISHGSVVAGVIGAKKPQYDIWGKTVNLASRMDSTGVSGRIQVPEETYLILKDQGFAFDYRGE IYVKGISEQEGKIKTYFLLGRVQPNPFILPPRRLPGQYSLAAVVLGLVQSLNRQRQKQLLNENSNSGIIK SHYNRRTLLTPSGPEPGAQAEGTDKSDLP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291903
ORF Size	3657 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291903.1, NP_001278832.1
RefSeq Size	4974 bp
RefSeq ORF	3660 bp
Locus ID	11514
MW	137.1 kDa
Gene Summary	Catalyzes the formation of cAMP in response to calcium entry leadings to cAMP signaling activation that affect processes suche as synaptic plasticity and insulin secretion (PubMed:10864938, PubMed:25403481, PubMed:10482244, PubMed:14585998, PubMed:18448650). Plays a role in many brain functions, such as learning, memory, drug addiction, and anxiety modulation through regulation of synaptic plasticity by modulating long-term memory and long-term potentiation (LTP) through CREB transcription factor activity modulation (PubMed:10482244, PubMed:14585998, PubMed:18448650, PubMed:10864938, PubMed:12441059, PubMed:20638449, PubMed:27234425, PubMed:18222416). Plays a central role in insulin secretion by controlling glucose homeostasis through glucagon-like peptide 1 and glucose signaling pathway and maintains insulin secretion through calcium-dependent PKA activation leading to vesicle pool replenishment (PubMed:25403481). Also, allows PTGER3 to induce potentiation of PTGER4-mediated PLA2 secretion by switching from a negative to a positive regulation, during the IL1B induced-dedifferentiation of smooth muscle cells (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.