Hdac9 (NM_001271386) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AV022454; D030072B18Rik; HD7B; HD9; Hdac7b; HDRP; Mitr; mKIAA0744
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231566 representing NM_001271386 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTCGCCGGTGCAGCCAGATGGAGTGGCCGGACGGGAGCAGCTCTTGGCTCAGCAAAGAATGCACA GTATGATCAGCTCAGTGGACGTGAAATCAGAGGTTCCTATGGGCCTGGAGCCCATCTCACCTTTAGACCT GAGGACAGATCTCAGGATGATGATGCCTGTGGTGGATCCTGTCGTCCGCGAGAAGCAACTGCAGCAAGAG TTACTTCTTATTCAGCAGCAGCAGCAGATCCAGAAGCAGCTCCTCATAGCAGAGTTTCAGAAGCAGCACG AGAATTTGACAAGGCAGCATCAAGCACAACTCCAGGAGCACATCAAGTTACAACAGGAACTTCTAGCCAT AAAACAACAGCAAGAACTCCTAGAAAAGGAGCAGAAACTGGAGCAGCAGAGGCAAGAACAGGAAGTAGAG AGGCATCGCAGAGAGCAGCAGCTTCCTCCTCTCAGAGGCAAAGATAGAGGACGAGAAAGGGCAGTGGCAA GCACAGAGGTTAAGCAGAAGCTTCAAGAATTCCTGTTGAGTAAATCAGCAACAAAAGACACTCCAACCAA TGGAAAGAATCATTCCGTGGGCCGCCATCCCAAGCTCTGGTACACGGCTGCCCACCACACATCACTGGAT CAAAGCTCTCCACCCCTCAGTGGAACGTCTCCATCCTACAAGTACACATTACCAGGAGCTCAAGATAGCA AGGATGATTTCCCCTTGAGAAAAACTGCCTCAGAGCCCAACTTGAAGGTGCGGTCCAGGTTAAAACAGAA AGTGGCAGAGAGGAGAAGCAGTCCCTTACTCAGGCGGAAGGATGGAAATCTTGTCACTTCATTCAAGAAG CGAGTGTTTGAGGTGGCAGAATCCTCGGTCAGTAGCAGCTCTCCAGGGTCAGGTCCCAGTTCACCAAACA ATGGCCCTGCTGGGAATGTGACCGAAAATGAGGCTTCAGCTCTGCCTCCCACGCCTCACCCCGAGCAACT GGTTCCACAGCAGCGCATACTAATTCATGAAGATTCCATGAACCTGCTAAGTCTCTATACCTCCCCGTCC CTGCCCAATATCACTCTGGGACTTCCAGCAGTGCCGTCCCCACTCAATGCTTCTAACTCACTCAAAGACA AACAGAAGTGCGAGACACAGATGCTCAGACAAGGTGTTCCTCTGCCCAGTCAGTATGGCAGTAGCATTGC AGCGTCCTCCAGCCACGTTCATGTAGCAATGGAAGGAAAACCCACCAGCAGCCACCAGGCTCTCCTGCAG CACCTACTGCTGAAGGAACAAATGCGACAGCAAAAGCTTCTCGTGGCTGGTGGAGTTCCATTACACCCTC AGTCTCCTTTGGCAACAAAAGAAAGAATTTCACCAGGCATTAGAGGTACCCACAAATTGCCCCGCCACCG ACCCCTGAATCGAACCCAGTCTGCACCTTTGCCTCAGAGCACGCTGGCTCAGCTGGTCATTCAGCAGCAG CACCAGCAGTTCCTGGAGAAGCAGAAACAATACCAGCAGCAGATCCACATGAACAAACTGCTATCGAAAT CTATTGAACAACTGAAGCAACCAGGCAGCCACCTTGAAGAAGCAGAGGAGGAGCTTCAGGGGGACCAATC CATGGAAGACAGAGCAGCCTCTAAGGACAACAGTGCTAGGAGTGACAGCAGTGCTTGTGTGGAGGACACG CTGGGACAAGTTGGGGCTGTGAAGGTCAAGGAGGAACCCGTGGACAGCGATGAAGATGCTCAGATACAGG AAATGGAATGTGGGGAGCAGGCTGCTTTTATGCAACAGCCTCCCTTGCTGGAGTCCTCACGCGCGCGCTC ACTCTCCGTGCACCAGACTCTGGCTGCAGTTGGCACCGATGGATTAGAGAAACATGGTCTCCTCTCCCGG ACCCATTCTTCTCCTGCAGCCTCCATGTCACCTCACCCAGCAGCCGACTGTCCCCGCCAGCCTTCCTCTG CCACTGGAATTGCCTATGACCCTCTGATGCTGAAACACCAGTGCATCTGTGGCAGCTCCACGACCCACCC TGAGCACGCCGGGCGGATACAGAGCATCTGGTCACGGCTGCAAGAAACGGGGCTGCTAAGTAAATGTGAG CGAATTCAAGGTCGAAAAGCCAGCCTGGAGGAAATACAGCTTGTTCATTCTGAACATCACTCACTATTGT ATGGCACCAGCCCCCTGGATGGACAGAAGCTGGACCCCAGGACACTCTTAGGTGATGATTCTCGGAAATT CTTTTCCTCGTTGCCTTGTGGTGGACTCGGGGTGGACAGTGACACCATTTGGAATGAGCTGCACTCGTCC GGTGCTGCACGCATGGCTGTTGGCTGTGTCATTGAGCTGGCTTCCAAAGTGGCCTCAGGAGAGCTGAAGA ATGGGTTTGCCGTTGTGAGGCCCCCAGGCCATCATGCGGAAGAGTCTGCAGCCATGGGGTTCTGCTTTTT TAATTCAGTTGCAATTACCGCCAAATACTTGAGAGACCAACTAAATATAAGCAAGATATTGATTGTAGAT CTGGATGTTCACCATGGAAACGGTACACAGCAGGCCTTTTATGCTGACCCCAGCATCCTGTACATTTCAC TCCATCGCTATGATGAAGGGAACTTTTTCCCTGGCAGTGGAGCCCCGAATGAGGTTGGAGTAGGTCTGGG AGAAGGCTACAATGTAAACATTGCCTGGACAGGTGGCCTCGACCCCCCTATGGGAGATGTTGAGTACCTT GAAGCGTTCAGGACTGTCGTGATGCCTGTGGCCAGAGAGTTTGATCCAGATATGGTCCTGGTTTCTGCTG GATTTGATGCATTGGAAGGCCATACCCCTCCCCTCGGGGGTTACAAAGTGACAGCAAAATGCTTTGGTCA TTTGACGAAGCAACTGATGACATTGGCTAATGGACGTGTGGCATTGGCTCTGGAAGGAGGACATGATCTG ACAGCTATTTGTGATGCATCGGAAGCCTGCATAAATGCCCTTCTAGGAAATGAGCCTGGTCCTCTTGAGG AAGATGCTCTCCACCAGTCCGTGAATACGAATGCAGCCGCTTCTTTACAGAAGACCATTGACATCCAAAG CAAGTATTGGAAGTCAATCAAGATGGTGGCTGCTGCAAGGGGCGGTGCTCTGCCTGCTTCTCAGCTGCAA GAGGAGACAGAGACCGTTTCTGCGCTAGCCTCCCTGACGGTAGATGTGGAACAGCCATTTGCTCAGGAAG ATGGCAGAACTGCTGGTGAGCCCATGGAAGAGGAGCCAGCCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231566 representing NM_001271386 Red=Cloning site Green=Tags(s) MSSPVQPDGVAGREQLLAQQRMHSMISSVDVKSEVPMGLEPISPLDLRTDLRMMMPVVDPVVREKQLQQE LLLIQQQQQIQKQLLIAEFQKQHENLTRQHQAQLQEHIKLQQELLAIKQQQELLEKEQKLEQQRQEQEVE RHRREQQLPPLRGKDRGRERAVASTEVKQKLQEFLLSKSATKDTPTNGKNHSVGRHPKLWYTAAHHTSLD QSSPPLSGTSPSYKYTLPGAQDSKDDFPLRKTASEPNLKVRSRLKQKVAERRSSPLLRRKDGNLVTSFKK RVFEVAESSVSSSSPGSGPSSPNNGPAGNVTENEASALPPTPHPEQLVPQQRILIHEDSMNLLSLYTSPS LPNITLGLPAVPSPLNASNSLKDKQKCETQMLRQGVPLPSQYGSSIAASSSHVHVAMEGKPTSSHQALLQ HLLLKEQMRQQKLLVAGGVPLHPQSPLATKERISPGIRGTHKLPRHRPLNRTQSAPLPQSTLAQLVIQQQ HQQFLEKQKQYQQQIHMNKLLSKSIEQLKQPGSHLEEAEEELQGDQSMEDRAASKDNSARSDSSACVEDT LGQVGAVKVKEEPVDSDEDAQIQEMECGEQAAFMQQPPLLESSRARSLSVHQTLAAVGTDGLEKHGLLSR THSSPAASMSPHPAADCPRQPSSATGIAYDPLMLKHQCICGSSTTHPEHAGRIQSIWSRLQETGLLSKCE RIQGRKASLEEIQLVHSEHHSLLYGTSPLDGQKLDPRTLLGDDSRKFFSSLPCGGLGVDSDTIWNELHSS GAARMAVGCVIELASKVASGELKNGFAVVRPPGHHAEESAAMGFCFFNSVAITAKYLRDQLNISKILIVD LDVHHGNGTQQAFYADPSILYISLHRYDEGNFFPGSGAPNEVGVGLGEGYNVNIAWTGGLDPPMGDVEYL EAFRTVVMPVAREFDPDMVLVSAGFDALEGHTPPLGGYKVTAKCFGHLTKQLMTLANGRVALALEGGHDL TAICDASEACINALLGNEPGPLEEDALHQSVNTNAAASLQKTIDIQSKYWKSIKMVAAARGGALPASQLQ EETETVSALASLTVDVEQPFAQEDGRTAGEPMEEEPAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271386
ORF Size	3264 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271386.1, NP_001258315.1
RefSeq Size	8195 bp
RefSeq ORF	3267 bp
Locus ID	79221
MW	119.5 kDa
Gene Summary	Devoided of intrinsic deacetylase activity, promotes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) by recruiting HDAC1 and HDAC3. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription, inhibits skeletal myogenesis and may be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.[UniProtKB/Swiss-Prot Function]

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