Tbc1d1 (NM_001289514) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1110062G02Rik; AI385682; AW555803; mKIAA1108; Nob1; Nobq1; Tbc1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231504 representing NM_001289514 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCACCATTGTTCAGCTGGGTGGCCAAGGTGCCTGAGATCATCAGCTCCATCCGGCAGGCCGGGAAGA TTGCCCGCCAGGAAGAGCTGCGTTGCCCCTCCGAGTTCGACGATACCTTCGCCAAAAAGTTCGAGGTGCT CTTCTGTGGCCGGGTGACTGTGGCTCACAAGAAGGCCCCACCCGCACTGATTGACGAGTGTATCGAGAAG TTCAACCATGTGAGCTGTGGTCGCAGAACGGACTGGGAAGCGCCCACCGGGCAGCCATCAGCGCCTGGCC CCAGGCCCATGCGCAAATCCTTCTCACAGCCTGGACTGCGCTCGCTGGCCTTCAGGAAGGAGTTCCAGGA CGCTAGCCTCCGCAGTAGCACCTTTAGCTCCTTTGACAATGACATAGAGAACCACCTCATCGGTGGGCAC AATGTGGTTCAGCCCACAGACATGGAGGAGAACCGAACTATGCTGTTCACGATTGGCCAATCTGAAGTTT ACCTCATCAGTCCTGACACCAAAAAGATTGCACTGGAGAAAAATTTTAAGGAGATATCCTTTTGCTCTCA GGGCATCAGACATGTGGACCACTTTGGATTCATCTGCCGAGAGTGCTCGGGTGGCGGCAGTGGCGGCTTT CATTTTGTCTGTTACGTGTTCCAGTGCACAAATGAAGCTCTGGTTGACGAGATCATGATGACTCTGAAGC AGGCTTTCACGGTAGCTGCGGTGCAGCAGACGGCTAAGGCACCAGCCCAGCTCTGTGAGGGCTGCCCCTT GCAAGGCCTGCACAAGCTCTGCGAAAGGATAGAGGGAATGAATTCATCTAAAACCAAATTAGAACTCCAG AAGCACTTGACCACACTGACCAATCAGGAGCAGGCCACCATATTCGAGGAGGTTCAGAAATTGAGACCAA GAAACGAGCAGCGAGAGAATGAATTAATTATTTCTTTTCTGAGGTGCTTATATGAAGAGAAGCAAAAAGA GCACAGCCACACTGGGGAGCCAAAGCAGACACTACAGGTGGCAGCAGAGAATATTGGGAGTGACCTGCCA CCCAGTGCTAGCCGGTTCAGGTTAGATTCGCTGAAGAACAGAGCAAAGAGGTCCTTAACAGAGTCCCTAG AGAGCATTCTGTCCCGGGGTAATAAAGCCAGAGGCCTGCAGGACCATTCCGCCAGTGTGGATCTGGACAG CTCCACTTCTAGTACTCTAAGTAACACCAGCAAAGAGCTGTCCATGGGTGACAAGGAGGCCTTCCCCGTC TCTGAGACCTCCTTCAAGCTCCTTGGCTCCTCAGATGACCTGTCCAGTGACTCAGAGGGCCACATTGCAG AAGAGTCTGCCCTGTTGTCACCCCAGCAGGCGTTCAGAAGGAGAGCCAACACCCTGAGTCATTTCCCAGT AGAGTGCCCTGCGCCTCCAGAACCTGCCCAGAGCTCTCCAGGGGTCTCTCAAAGGAAACTCATGCGGTAC CACTCCGTGAGCACAGAGACGCCTCATGAACGCAAGGACTTTGAATCCAAAGCAAACCACCTGGGTGACA CAGATGGGACCCCCGTGAAGACCCGGCGGCACTCGTGGAGACAGCAGATATTCCTTCGAGTGGCCACTCC ACAGAAGGCTTGTGACTCCCCGAGCAGATATGAAGATTATTCCGAGCTGGGAGAGCTCCCTCCACGCTCC CCTTTAGAACCGGTGTGTGAGGACGGCCCATTTGGCCCAGTACAGGAAGAAAAGAGGAAGACGTCACGCG AGCTTCGAGAGCTGTGGAAAAAGGCCATCTTGCAGCAGATCCTGCTGCTCAGGATGGAGAAGGAGAATCA GAAGCTACAAGCCTCTGAAAACGATTTGCTGAACAAACGCCTCAAGCTTGACTATGAAGAAATCACTCCG TGTCTTAAAGAAGTCACTACAGTGTGGGAAAAGATGCTTAGCACTCCAGGAAGATCCAAAATTAAGTTTG ACATGGAAAAAGTGCACTCAGCTGTTGGGCAAGGTGTGCCACGTCATCACCGAGGTGAGATCTGGAAATT TCTAGCTGAGCAGTTCCACCTTAAACACCCATTTCCTAGTAAACAGCAGCCAAAGGACGTGCCCTACAAA GAGCTCCTGAAGAAGCTGACCTCGCAGCAGCACGCCATTCTCATCGACCTCGGGCGAACCTTTCCAACAC ATCCATACTTCTCTGCCCAGCTTGGAGCAGGTCAGCTGTCACTTTACAACATTCTGAAGGCCTACTCGCT TCTGGACCAGGAGGTTGGATACTGCCAAGGTCTCAGCTTTGTGGCAGGCATTTTGCTTCTTCACATGAGT GAGGAAGAGGCGTTCAAGATGCTCAAGTTCCTGATGTTTGACATGGGGCTGCGGAAACAGTATCGGCCAG ACATGATTATTTTGCAGATCCAGATGTACCAGCTGTCACGGCTCCTCCACGATTACCACCGAGACCTCTA CAACCACCTGGAAGAGCACGAGATCGGCCCCAGCCTCTACGCGGCTCCCTGGTTTCTCACTGTGTTCGCC TCACAGTTCCCACTCGGCTTTGTAGCCAGAGTCTTTGATATGATCTTCCTTCAGGGATCAGAGGTCATAT TTAAAGTAGCTTTAAGTCTTTTGGGGAGCCATAAGCCCTTGATTCTACAGCATGAGAACCTGGAAACCAT AGTGGACTTCATAAAGAACACACTCCCCAACCTGGGCCTGGTGCAGATGGAGAAGACCATCAGTCAGGTG TTTGAGATGGACATCGCCAAGCAGCTCCAGGCCTATGAGGTCGAGTACCACGTGCTCCAGGAGGAGCTTA TTGAGTCCTCGCCTCTCAGTGACAACCAAAGAATGGAGAAATTGGAGAAAACCAACAGCAGCTTGCGCAA ACAGAACCTTGACCTCCTGGAGCAGTTGCAGGTGGCAAATGCTAGGATCCAAAGCCTTGAAGCCACGGTA GAGAAACTTCTTACCAGCGAGAGTAAGCTGAAGCAGGCTGCGCTGACCCTGGAGGTGGAGCGCTCCGCCC TGCTGCAGATGGTGGAGGAGCTGCGGAGGCAAAGCGCCCGGCCCAGCACTCCAGAGCCAGACTGCACCCA GCTGGAGCCCACAGGCGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231504 representing NM_001289514 Red=Cloning site Green=Tags(s) MSPLFSWVAKVPEIISSIRQAGKIARQEELRCPSEFDDTFAKKFEVLFCGRVTVAHKKAPPALIDECIEK FNHVSCGRRTDWEAPTGQPSAPGPRPMRKSFSQPGLRSLAFRKEFQDASLRSSTFSSFDNDIENHLIGGH NVVQPTDMEENRTMLFTIGQSEVYLISPDTKKIALEKNFKEISFCSQGIRHVDHFGFICRECSGGGSGGF HFVCYVFQCTNEALVDEIMMTLKQAFTVAAVQQTAKAPAQLCEGCPLQGLHKLCERIEGMNSSKTKLELQ KHLTTLTNQEQATIFEEVQKLRPRNEQRENELIISFLRCLYEEKQKEHSHTGEPKQTLQVAAENIGSDLP PSASRFRLDSLKNRAKRSLTESLESILSRGNKARGLQDHSASVDLDSSTSSTLSNTSKELSMGDKEAFPV SETSFKLLGSSDDLSSDSEGHIAEESALLSPQQAFRRRANTLSHFPVECPAPPEPAQSSPGVSQRKLMRY HSVSTETPHERKDFESKANHLGDTDGTPVKTRRHSWRQQIFLRVATPQKACDSPSRYEDYSELGELPPRS PLEPVCEDGPFGPVQEEKRKTSRELRELWKKAILQQILLLRMEKENQKLQASENDLLNKRLKLDYEEITP CLKEVTTVWEKMLSTPGRSKIKFDMEKVHSAVGQGVPRHHRGEIWKFLAEQFHLKHPFPSKQQPKDVPYK ELLKKLTSQQHAILIDLGRTFPTHPYFSAQLGAGQLSLYNILKAYSLLDQEVGYCQGLSFVAGILLLHMS EEEAFKMLKFLMFDMGLRKQYRPDMIILQIQMYQLSRLLHDYHRDLYNHLEEHEIGPSLYAAPWFLTVFA SQFPLGFVARVFDMIFLQGSEVIFKVALSLLGSHKPLILQHENLETIVDFIKNTLPNLGLVQMEKTISQV FEMDIAKQLQAYEVEYHVLQEELIESSPLSDNQRMEKLEKTNSSLRKQNLDLLEQLQVANARIQSLEATV EKLLTSESKLKQAALTLEVERSALLQMVEELRRQSARPSTPEPDCTQLEPTGD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289514
ORF Size	3099 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289514.1, NP_001276443.1
RefSeq Size	4802 bp
RefSeq ORF	3102 bp
Locus ID	57915
MW	117.7 kDa
Gene Summary	May act as a GTPase-activating protein for Rab family protein(s). May play a role in the cell cycle and differentiation of various tissues. Involved in the trafficking and translocation of GLUT4-containing vesicles and insulin-stimulated glucose uptake into cells.[UniProtKB/Swiss-Prot Function]

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