Sox6 (NM_001277326) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI987981; SOX-LZ
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231223 representing NM_001277326 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTCCAAGCAAGCCACCTCTCCATTTGCCTGTACAGCGGATGGAGAGGAAGCAATGACCCAGGACT TGACCTCAAGGGAAAAGGAAGAGGGCAGTGATCAACACCCGGCCTCCCATCTGCCTCTGCACCCCATAAT GCACAACAAACCTCACTCTGAGGAGCTGCCTACACTTGTCAGTACCATTCAACAAGATGCTGACTGGGAC AGCGTTCTGTCATCTCAGCAAAGAATGGAGTCAGAGAATAATAAGTTATGTTCCCTATATTCCTTCCGAA ATACGTCTACCTCACCACATAAGCCTGACGAAGGGAGTCGGGAGCGTGAAATAATGAACAGTGTTACTTT TGGAACCCCGGAACGCCGCAAAGGGAGCCTTGCCGATGTGGTGGATACGCTGAAGCAGAAGAAGCTGGAG GAGATGACTCGGACAGAACAAGAGGATTCCTCCTGCATGGAAAAACTACTTTCAAAAGATTGGAAGGAAA AAATGGAAAGACTAAATACGAGTGAACTTCTTGGAGAAATCAAAGGTACACCTGAGAGCCTTGCAGAGAA AGAACGGCAACTCTCCACCATGATTACCCAGCTGATCAGCTTGCGGGAGCAGCTCCTGGCAGCCCATGAT GAACAGAAAAAGCTGGCAGCATCGCAGATCGAGAAACAGCGGCAGCAAATGGACCTTGCTCGCCAACAGC AAGAACAGATCGCAAGACAACAGCAGCAACTTCTACAGCAGCAGCACAAGATTAATCTCCTGCAGCAACA GATCCAGGTTCAGGGGCACATGCCTCCGCTCATGATCCCAATTTTTCCACATGACCAGCGGACCTTGGCA GCAGCTGCTGCTGCCCAACAGGGATTCCTCTTCCCCCCTGGAATAACATACAAGCCAGGTGATAACTACC CCGTACAGTTCATTCCGTCAACAATGGCAGCTGCTGCTGCTTCTGGACTCAGCCCTTTACAGCTTCAGAA GGGTCATGTTTCCCACCCACAAATTAACCCAAGGCTAAAGGGCATAAGTGACCGTTTTGGCAGGAATTTG GACCCCTCTGAACATGGTGGTGGCCACTCTTACAACCACAGACAGATTGAGCAGCTCTATGCCGCTCAGC TGGCCAGCATGCAGGTGTCACCTGGAGCAAAGATGCCATCAACTCCACAGCCACCAAACTCGGCAGGGGC AGTCTCACCTACTGGGATAAAAAATGAAAAGAGAGGGACCAGCCCTGTAACTCAAGTTAAGGATGAAACA ACAGCCCAGCCGCTGAATCTCTCATCCCGGCCTAAGACAGCAGAGCCTGTGAAGTCCCCAACATCTCCCA CCCAGAACCTCTTCCCAGCCAGCAAAACCAGCCCTGTCAACCTGCCAAACAAAAGCAGCATCCCCAGCCC CATTGGAGGAAGCCTGGGGAGAGGATCCTCTCTAGATATACTATCCAGTCTCAACTCTCCTGCCCTGTTT GGGGACCAGGACACAGTGATGAAAGCCATTCAGGAGGCTCGGAAGATGCGAGAACAGATCCAGCGGGAGC AACAACAGCAGCCACACGGAGTTGATGGGAAACTGTCCTCCATGAACAACATGGGGCTGAGCAACTGCAG GACTGAGAAGGAAAGAACACGCTTTGAGAACCTGGGTCCCCAATTAACAGGAAAGTCAAGTGAAGATGGA AAACTGGGCCCGGGTGTCATCGATCTCACTCGGCCAGAGGACGCAGAAGGAAGCAAAGCAATGAATGGCT CTGCAGCTAAACTACAGCAGTATTATTGTTGGCCAACAGGAGGCGCCACTGTGGCTGAAGCCCGTGTCTA CAGGGATGCCCGCGGCCGTGCCAGCAGCGAACCCCACATCAAGCGACCAATGAATGCGTTCATGGTTTGG GCAAAGGACGAAAGGAGGAAAATTCTTCAGGCCTTCCCTGACATGCATAACTCCAACATTAGCAAAATCT TAGGATCTCGCTGGAAATCCATGTCCAACCAGGAGAAGCAACCTTACTATGAAGAACAGGCCCGGCTAAG CAAAATCCACCTAGAGAAGTACCCAAACTACAAGTACAAACCCCGGCCGAAGCGCACATGCATCGTGGAT GGCAAGAAGCTCCGGATTGGGGAGTACAAGCAACTGATGCGCTCGCGAAGGCAGGAGATGCGACAGTTCT TCACTGTGGGGCAACAGCCTCAGATGCCCATCACCACAGGAACAGGTGTTGTGTATCCTGGTGCTATTAC TATGGCAACGACCACACCATCACCTCAGATGACATCTGACTGCTCTAGCACCTCTGCCAGCCCCGAGCCC AGCCTCCCTGTCATCCAGAGCACGTATGGTATGAAGATGGACGGCGCAAGCCTGGCTGGGAACGACATGA TTAATGGAGAGGATGAAATGGAAGCCTATGACGACTACGAAGATGATCCCAAATCAGACTATAGCAGCGA GAATGAGGCCCCAGAGCCTGTCAGTGCCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231223 representing NM_001277326 Red=Cloning site Green=Tags(s) MSSKQATSPFACTADGEEAMTQDLTSREKEEGSDQHPASHLPLHPIMHNKPHSEELPTLVSTIQQDADWD SVLSSQQRMESENNKLCSLYSFRNTSTSPHKPDEGSREREIMNSVTFGTPERRKGSLADVVDTLKQKKLE EMTRTEQEDSSCMEKLLSKDWKEKMERLNTSELLGEIKGTPESLAEKERQLSTMITQLISLREQLLAAHD EQKKLAASQIEKQRQQMDLARQQQEQIARQQQQLLQQQHKINLLQQQIQVQGHMPPLMIPIFPHDQRTLA AAAAAQQGFLFPPGITYKPGDNYPVQFIPSTMAAAAASGLSPLQLQKGHVSHPQINPRLKGISDRFGRNL DPSEHGGGHSYNHRQIEQLYAAQLASMQVSPGAKMPSTPQPPNSAGAVSPTGIKNEKRGTSPVTQVKDET TAQPLNLSSRPKTAEPVKSPTSPTQNLFPASKTSPVNLPNKSSIPSPIGGSLGRGSSLDILSSLNSPALF GDQDTVMKAIQEARKMREQIQREQQQQPHGVDGKLSSMNNMGLSNCRTEKERTRFENLGPQLTGKSSEDG KLGPGVIDLTRPEDAEGSKAMNGSAAKLQQYYCWPTGGATVAEARVYRDARGRASSEPHIKRPMNAFMVW AKDERRKILQAFPDMHNSNISKILGSRWKSMSNQEKQPYYEEQARLSKIHLEKYPNYKYKPRPKRTCIVD GKKLRIGEYKQLMRSRRQEMRQFFTVGQQPQMPITTGTGVVYPGAITMATTTPSPQMTSDCSSTSASPEP SLPVIQSTYGMKMDGASLAGNDMINGEDEMEAYDDYEDDPKSDYSSENEAPEPVSAN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001277326
ORF Size	2481 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001277326.1, NP_001264255.1
RefSeq Size	8662 bp
RefSeq ORF	2484 bp
Locus ID	20679
UniProt ID	P40645
MW	92.3 kDa
Gene Summary	This gene encodes a member of a family of transcriptional regulators containing high mobility group (HMG) DNA-binding domains. Function of the encoded protein is important for proper cardiac and skeletal development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]

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