Brd2 (NM_001204973) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AW228947; D17H6S113E; Frg-1; Fsrg-1; Fsrg1; mKIAA4005; Nat; Ring3; Rnf3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231166 representing NM_001204973 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCAAAACGTGACTCCCCACAAGCTCCCTGGGGAAGGGAATGCAGGGTTACTGGGGCTGGGCCCAG AGGCAGCAGCACCAGGGAAAAGGATTCGAAAGCCTTCTCTGCTGTATGAGGGATTTGAGAGCCCCACAAT GGCTTCTGTACCAGCTTTACAACTGGCCCCTGCCAATCCACCACCCCCTGAGGTGTCCAATCCCAAAAAG CCAGGACGGGTAACAAACCAACTGCAGTACCTGCACAAGGTAGTGATGAAGGCTCTGTGGAAGCATCAGT TTGCATGGCCATTCCGGCAGCCTGTGGACGCTGTGAAGCTGGGTTTGCCGGATTATCACAAAATTATAAA ACAGCCTATGGACATGGGTACTATCAAGAGGAGACTTGAAAACAATTACTACTGGGCTGCCTCAGAATGT ATGCAGGATTTTAATACTATGTTTACCAACTGTTACATTTATAACAAGCCCACCGATGATATTGTCCTAA TGGCACAGACACTGGAAAAGATCTTCCTACAGAAAGTAGCATCCATGCCACAAGAGGAGCAAGAGCTTGT GGTGACCATCCCTAAAAACAGCCATAAGAAGGGGGCCAAGTTAGCAGCACTCCAGGGCAGTATTACCAGT GCCCATCAGGTGCCTGCTGTCTCTTCTGTGTCGCATACAGCCCTGTATACACCACCACCTGAAATACCTA CCACTGTCCTCAACATTCCCCACCCATCAGTCATCTCTTCTCCTCTTCTTAAGTCCCTGCATTCTGCTGG ACCCCCACTCCTTGCTGTATCAGCAGCGCCTCCAGCTCAGCCCCTTGCCAAGAAAAAAGGCGTTAAACGG AAAGCGGATACTACCACCCCTACACCCACAGCCATCCTGGCTCCTGGTTCCCCAGCTAGTCCTCCTGGGA GTCTTGAGCCAAAGGCAGCAAGGCTCCCTCCTATGCGCAGAGAGAGTGGCCGCCCAATCAAACCCCCACG AAAAGACTTGCCTGACTCGCAACAGCAACACCAGAGCTCTAAGAAAGGGAAGCTGTCAGAGCAGTTAAAG CACTGCAACGGCATCCTGAAGGAACTGCTCTCAAAGAAGCACGCTGCCTACGCCTGGCCCTTCTATAAGC CAGTGGACGCTTCTGCTCTTGGCCTTCATGATTACCATGACATCATTAAACACCCCATGGACCTCAGCAC TGTCAAGCGGAAGATGGAGAACCGTGACTACCGGGATGCACAGGAGTTTGCTGCTGATGTACGGCTTATG TTCTCCAACTGCTATAAGTACAATCCTCCAGACCACGATGTTGTGGCTATGGCACGAAAGTTGCAGGATG TGTTTGAGTTTCGCTATGCCAAGATGCCAGATGAGCCACTGGAACCAGGACCTCTACCAGTCTCTACTGC CTTGCCTCCTGGGTTGACCAAATCCTCTTCAGAGTCCTCCAGTGAGGAAAGTAGCAGTGAGAGTTCCTCT GAGGAAGAGGAGGAGGAGGAAGAAGATGAGGACGAGGAGGAGAGTGAAAGCTCAGACTCTGAGGAGGAAA GGGCTCATCGCCTAGCAGAGCTGCAGGAGCAGCTTCGGGCAGTTCATGAACAACTGGCTGCCCTGTCCCA GGGCCCAATATCTAAGCCCAAGCGGAAGAGAGAGAAAAAGGAAAAAAAGAAGAAACGGAAGGCAGAGAAA CATCGTGGCCGAATTGGGATCGATGAAGATGATAAGGGGCCTAGGGCACCTCGCCCACCTCAGCCCAAGA AATCTAAGAAAGCAGGTGGTGGGGGTAGCAATGCTACTACACTCAGCCATCCTGGCTTTGGGACTTCCGG AGGAAGTAGCAACAAGCTACCTAAAAAGTCTCAAAAGACAGCTCCACCTGTCCTTCCCACTGGCTATGAT TCTGAGGAGGAGGAAGAAAGCAGGCCCATGAGTTATGATGAGAAGAGACAGTTAAGCCTGGATATCAATA AGTTACCTGGGGAAAAGCTGGGTCGAGTAGTACATATCATCCAAGCCAGGGAACCCTCTCTACGTGATTC AAATCCAGAAGAAATTGAGATTGATTTTGAAACACTCAAGCCGTCCACACTTAGAGAGCTTGAGCGATAT GTTTTATCCTGCCTTCGAAAGAAACCCCGGAAGCCCTACACTATTAGGAAACCTGTGGGAAAAACAAAGG AGGAACTGGCTTTGGAGAAGAAGCGGGAGCTAGAGAAGCGGTTGCAGGATGTCAGTGGACAGCTCAACTC CACCAAAAAGCCTCCCAAGAAAGCGAGTGAGAAGACAGAGTCATCTGCACAGCAAGTGGCAGTGTCCCGT CTCAGTGCTTCTAGTTCCAGCTCAGATTCCAGCTCCTCGTCGTCATCTTCCTCTTCTTCAGACACCAGCG ATTCAGACTCGGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231166 representing NM_001204973 Red=Cloning site Green=Tags(s) MLQNVTPHKLPGEGNAGLLGLGPEAAAPGKRIRKPSLLYEGFESPTMASVPALQLAPANPPPPEVSNPKK PGRVTNQLQYLHKVVMKALWKHQFAWPFRQPVDAVKLGLPDYHKIIKQPMDMGTIKRRLENNYYWAASEC MQDFNTMFTNCYIYNKPTDDIVLMAQTLEKIFLQKVASMPQEEQELVVTIPKNSHKKGAKLAALQGSITS AHQVPAVSSVSHTALYTPPPEIPTTVLNIPHPSVISSPLLKSLHSAGPPLLAVSAAPPAQPLAKKKGVKR KADTTTPTPTAILAPGSPASPPGSLEPKAARLPPMRRESGRPIKPPRKDLPDSQQQHQSSKKGKLSEQLK HCNGILKELLSKKHAAYAWPFYKPVDASALGLHDYHDIIKHPMDLSTVKRKMENRDYRDAQEFAADVRLM FSNCYKYNPPDHDVVAMARKLQDVFEFRYAKMPDEPLEPGPLPVSTALPPGLTKSSSESSSEESSSESSS EEEEEEEEDEDEEESESSDSEEERAHRLAELQEQLRAVHEQLAALSQGPISKPKRKREKKEKKKKRKAEK HRGRIGIDEDDKGPRAPRPPQPKKSKKAGGGGSNATTLSHPGFGTSGGSSNKLPKKSQKTAPPVLPTGYD SEEEEESRPMSYDEKRQLSLDINKLPGEKLGRVVHIIQAREPSLRDSNPEEIEIDFETLKPSTLRELERY VLSCLRKKPRKPYTIRKPVGKTKEELALEKKRELEKRLQDVSGQLNSTKKPPKKASEKTESSAQQVAVSR LSASSSSSDSSSSSSSSSSSDTSDSDSG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001204973
ORF Size	2394 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001204973.1, NP_001191902.1
RefSeq Size	4685 bp
RefSeq ORF	2397 bp
Locus ID	14312
UniProt ID	Q7JJ13
MW	88.1 kDa
Gene Summary	Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling. Regulates transcription of the CCND1 gene. Plays a role in nucleosome assembly (By similarity). May play a role in spermatogenesis or folliculogenesis.[UniProtKB/Swiss-Prot Function]

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