P2rx7 (NM_001284402) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI467586; P2X(7); P2X7R
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230613 representing NM_001284402 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCCCGTGAGCCACTTATGCAGCCATAAATCCGGGAAAGTCCTTGAAATCCATAGCATATGGATCG GGACGCTGAAGAACACCTTCCTAGGGGCCATCTGCGTCTATATCTGCTTTGCTTTGGTGAGCGATAAGCT GTACCAGCGGAAAGAGCCTGTTATCAGCTCCGTGCACACCAAGGTCAAAGGCATAGCAGAGGTGACGGAG AATGTCACAGAGGGTGGGGTGACGAAGTTAGGACACAGCATCTTTGACACTGCAGACTACACCTTCCCTT TGCAGGGGAACTCATTCTTTGTCATGACAAACTATGTCAAGTCAGAAGGCCAAGTGCAGACGCTGTGTCC TGAGTATCCCAGGCGCGGTGCACAGTGCTCTTCTGACCGGCGTTGTAAAAAGGGGTGGATGGACCCACAG AGCAAAGGAATCCAGACTGGCAGGTGTGTTCCATATGACAAGACAAGGAAGACCTGTGAAGTCTCTGCCT GGTGTCCTACTGAGGAGGAGAAAGAAGCTCCCCGACCTGCACTCTTGAGGAGCGCCGAAAACTTCACCGT ACTCATCAAGAATAATATCCACTTCCCCGGCCACAACTATACCACGAGAAACATCTTGCCAACTATGAAC GGCTCTTGTACCTTTCACAAGACTTGGGACCCTCAGTGTTCCATCTTCCGACTAGGGGACATCTTCCAGG AAGCAGGAGAGAACTTTACAGAGGTGGCAGTTCAGGGAGGAATCATGGGTATCGAGATCTACTGGGATTG CAACCTAGACAGCTGGTCCCATCACTGCCGACCCAGGTACAGCTTCCGCCGCCTGGATGACAAGAACACG GATGAGTCCTTCGTCCCCGGCTACAACTTCAGATATGCCAAGTACTATAAGGAGAACAATGTGGAAAAGC GGACGTTGATCAAAGCCTTCGGCATCCGTTTTGACATCCTGGTTTTCGGCACTGGAGGAAAATTTGACAT CATCCAGCTGGTTGTATACATTGGATCCACCCTGTCCTACTTTGGCTTGGCCACTGTGTGCATTGACTTG CTCATCAACACATACTCCAGTGCTTTCTGCAGGTCGGGGGTTTACCCCTACTGTAAGTGCTGTGAGCCCT GCACAGTGAACGAGTATTACTACAGAAAGAAGTGTGAGTCCATCATGGAACCCAAGCCGACGTTGAAGTA TGTGTCCTTTGTCGACGAGCCGCACATTCGCATGGTGGACCAGCAGCTGCTTGGGAAAAGTCTGCAAGTT GTCAAAGGCCAAGAAGTTCCAAGACCCCAGATGGACTTCTCCGACCTGTCTAGGCTGTCCCTATCTCTCC ACGACTCACCCCTGACTCCTGGACAATCTGAGGAAATTCAGCTGCTCCATGAAGAGGTGGCCCCTAAGTC CGGGGACAGCCCGAGTTGGTGCCAGTGTGGAAATTGCCTCCCGTCTCGCCTACCGGAGCAACGCAGGGCC CTGGAGGAGCTGTGCTGCCGGAGGAAGCCGGGGCGGTGCATCACCACCTCCAAGCTCTTCCATAAGCTCG TGCTGTCCCGAGACACCCTGCAGCTCCTCCTGCTTTACCAGGATCCCTTGCTGGTGCTGGGGGAAGAGGC CACCAACAGCAGGCTGCGACACCGTGCTTACAGGTGCTATGCCACCTGGCGCTTCGGCTCCCAGGACATG GCCGACTTTGCCATTCTGCCCAGCTGTTGCCGCTGGAGGATCCGGAAGGAGTTCCCCAAGACCGAGGGGC AGTATAGTGGCTTCAAGTATCCCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230613 representing NM_001284402 Red=Cloning site Green=Tags(s) MLPVSHLCSHKSGKVLEIHSIWIGTLKNTFLGAICVYICFALVSDKLYQRKEPVISSVHTKVKGIAEVTE NVTEGGVTKLGHSIFDTADYTFPLQGNSFFVMTNYVKSEGQVQTLCPEYPRRGAQCSSDRRCKKGWMDPQ SKGIQTGRCVPYDKTRKTCEVSAWCPTEEEKEAPRPALLRSAENFTVLIKNNIHFPGHNYTTRNILPTMN GSCTFHKTWDPQCSIFRLGDIFQEAGENFTEVAVQGGIMGIEIYWDCNLDSWSHHCRPRYSFRRLDDKNT DESFVPGYNFRYAKYYKENNVEKRTLIKAFGIRFDILVFGTGGKFDIIQLVVYIGSTLSYFGLATVCIDL LINTYSSAFCRSGVYPYCKCCEPCTVNEYYYRKKCESIMEPKPTLKYVSFVDEPHIRMVDQQLLGKSLQV VKGQEVPRPQMDFSDLSRLSLSLHDSPLTPGQSEEIQLLHEEVAPKSGDSPSWCQCGNCLPSRLPEQRRA LEELCCRRKPGRCITTSKLFHKLVLSRDTLQLLLLYQDPLLVLGEEATNSRLRHRAYRCYATWRFGSQDM ADFAILPSCCRWRIRKEFPKTEGQYSGFKYPY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001284402
ORF Size	1776 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284402.2
RefSeq Size	4764 bp
RefSeq ORF	1779 bp
Locus ID	18439
MW	68.2 kDa
Gene Summary	Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. In the absence of its natural ligand, ATP, functions as a scavenger receptor in the recognition and engulfment of apoptotic cells.[UniProtKB/Swiss-Prot Function]

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