Vrk2 (NM_001252447) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2810003O05Rik; AI447698
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229988 representing NM_001252447 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACCAAGAAGAAAAGAGAAATATAAGCTTCCGGTTCCACTCCCAGAAGGCAAAATTCTGGATGATA TGGAAGGAAACCGGTGGGCACTGGGCAAGATGATCGGCTCTGGAGGGTTTGGACTGATATACTTAGCTTT CCCCACAAATAAACCAAACAAAGATGCAAGACATGTCATAAAGCTGGAGTATCAAGAAAATGGCCCATTA TTTTCAGAGCTTAAATTTTATCAGAGAGCTGCAAAAAGAGAATGTATCCAAAAGTGGATACAACAGAGGA AACTTGATTATTTAGGAATTCCTGTATTTTATGGATTTGGTCTGACTGATTTCAAAGGAAGAAGTTACAG ATTCATGGTAATGGAAAGACTTGGAATAGATTTACAGAAACTCCTGGACCAGAATGGTGGTTTTAAAAAA TTAACCGTCCTACAACTTGGCATCAGGATGCTGGATGTACTGGAATATATACATGAAAATGAGTATGTTC ATGGTGATATAAAAGCCGCAAATCTACTGTTGGATTTTACAAATCCAGACCGGGTTTATCTTGCAGACTA TGGACTTTCCTACAGATATTGTCCCAATGGGAACCACAAACAGTATCAGGAAGATCCCAGAAAGGGCCAT AATGGGACAATAGAGTTTACAAGTTTGGATGCACACAAAGGAGTGGCCCCGTCCAGGAGGAGTGATGTTG AAATCCTTGGTTACTGCATGCTGCACTGGCTCTTCGGGAAGCTTCCTTGGGAAGCAAAGCTGGACGACCC TGTGGCTGTCCAGACTGCTAAAACCAACCTGCTGGATGAACTCCCAGAGTCGGTGCTTAAGTGGGCTCCT TCTGGAAGCAGTTGCAGTGAACTTGTCAAGTACTTGATGTATGTTCATAATTTAGCTTATGATGACAAGC CAGACTATCAGAAGCTCAAGAAAATTTTGAATCCAGATGGAGTACCTTTAGGGCCACTGGAATTTTCCAC TAAAGTACAGAGTGTCCATGTGCGGACTCCAGCCCAACAAAAAGAAAACTCAAGGACAAGGAAAATACAT GAATATTCTGATATTTTCAGTGAGATGCAGAGTCTTCAACAAACACCCAGCTATATGAGTTTCCAAGGTT CATATTGCAAGCCCTATCTGGACTGCACTAGAAGAGATCCCATCAGGAAGCCAAGATCCCTGCCTCGGTA CAGACACACGCCCACGGGTAATCTGGGAGTCACAGACTTGGAAAGTTCTCCAAGGTTTTGGCCTGCAATT TTTCAGTTGACTCTGAGTGAAGAGACAAAGGCAGATGTGTATTACTATGGGATCACTATATTCTGTCTGC TGATATTTGTATTTCTTGCTTTGTATTTTCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229988 representing NM_001252447 Red=Cloning site Green=Tags(s) MAPRRKEKYKLPVPLPEGKILDDMEGNRWALGKMIGSGGFGLIYLAFPTNKPNKDARHVIKLEYQENGPL FSELKFYQRAAKRECIQKWIQQRKLDYLGIPVFYGFGLTDFKGRSYRFMVMERLGIDLQKLLDQNGGFKK LTVLQLGIRMLDVLEYIHENEYVHGDIKAANLLLDFTNPDRVYLADYGLSYRYCPNGNHKQYQEDPRKGH NGTIEFTSLDAHKGVAPSRRSDVEILGYCMLHWLFGKLPWEAKLDDPVAVQTAKTNLLDELPESVLKWAP SGSSCSELVKYLMYVHNLAYDDKPDYQKLKKILNPDGVPLGPLEFSTKVQSVHVRTPAQQKENSRTRKIH EYSDIFSEMQSLQQTPSYMSFQGSYCKPYLDCTRRDPIRKPRSLPRYRHTPTGNLGVTDLESSPRFWPAI FQLTLSEETKADVYYYGITIFCLLIFVFLALYFL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001252447
ORF Size	1362 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252447.1, NP_001239376.1
RefSeq Size	1745 bp
RefSeq ORF	1365 bp
Locus ID	69922
UniProt ID	Q8BN21
MW	52.9 kDa
Gene Summary	Serine/threonine kinase that regulates several signal transduction pathways. Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta (IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase (MAPK) complexes. Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription. Phosphorylates histone H3. Phosphorylates 'Thr-18' of p53/TP53, and thereby increases its stability and activity. Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins. Downregulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1. Blocks the phosphorylation of ERK in response to ERBB2 and HRAS. May also phosphorylate MAPK8IP1. Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity: casein, MBP and histone H2B, but it is not sure that this is physiologically relevant (By similarity).[UniProtKB/Swiss-Prot Function]

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