Trex1 (NM_001012236) Mouse Tagged ORF Clone
CAT#: MR229187
- TrueORF®
Trex1 (myc-DDK-tagged) - Mouse three prime repair exonuclease 1 (Trex1), transcript variant 2
"NM_001012236" in other vectors (1)
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CNY 2,640.00
CNY 3,810.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AU041952 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR229187 representing NM_001012236.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGCTCACAGACCCTGCCCCATGGTCACATGCAGACCCTCATCTTCTTAGACCTGGAAGCCACTGGC CTGCCTTCGTCTCGGCCCGAAGTCACAGAGCTGTGCCTGCTGGCTGTCCACAGACGTGCTCTGGAGAAC ACTTCCATTTCTCAGGGACATCCACCTCCAGTGCCCAGACCGCCCCGTGTGGTGGACAAGCTCTCTCTG TGCATTGCTCCAGGGAAAGCCTGTAGCCCTGGGGCCAGTGAGATCACAGGTCTGAGCAAAGCTGAGCTG GAAGTACAGGGGCGTCAACGCTTCGATGACAACCTGGCCATCCTGCTCCGAGCCTTCCTGCAGCGCCAG CCACAGCCTTGCTGCCTTGTGGCACACAACGGTGACCGCTATGACTTTCCTCTGCTCCAGACAGAGCTT GCTAGGCTGAGCACTCCCAGTCCCCTAGATGGTACCTTCTGTGTGGACAGCATCGCTGCCCTAAAGGCC TTGGAACAAGCTAGCAGCCCCTCAGGGAATGGTTCGAGGAAAAGCTACAGCCTGGGCAGCATCTACACC CGCCTGTACTGGCAAGCACCGACAGACTCACATACTGCTGAAGGTGATGTTCTAACCCTGCTCAGCATC TGTCAGTGGAAGCCACAGGCCCTACTGCAGTGGGTGGACGAACATGCCCGGCCCTTTAGCACCGTCAAG CCCATGTACGGCACTCCGGCTACCACTGGAACAACCAACCTAAGGCCACATGCTGCCACAGCTACTACA CCCCTGGCCACAGCCAATGGAAGTCCCAGCAATGGCAGGAGCAGGCGACCTAAGAGTCCTCCTCCAGAG AAGGTCCCAGAAGCCCCATCACAGGAGGGGCTGCTGGCCCCACTGAGCCTGCTGACCCTCCTGACCTTG GCAATAGCCACTCTGTATGGACTCTTCCTGGCCTCACCTGGGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by MR229187
Blue=ORF Red=Cloning site Green=Tag(s) MGSQTLPHGHMQTLIFLDLEATGLPSSRPEVTELCLLAVHRRALENTSISQGHPPPVPRPPRVVDKLSL CIAPGKACSPGASEITGLSKAELEVQGRQRFDDNLAILLRAFLQRQPQPCCLVAHNGDRYDFPLLQTEL ARLSTPSPLDGTFCVDSIAALKALEQASSPSGNGSRKSYSLGSIYTRLYWQAPTDSHTAEGDVLTLLSI CQWKPQALLQWVDEHARPFSTVKPMYGTPATTGTTNLRPHAATATTPLATANGSPSNGRSRRPKSPPPE KVPEAPSQEGLLAPLSLLTLLTLAIATLYGLFLASPGQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001012236 |
ORF Size | 942 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001012236.1, NP_001012236.1 |
RefSeq Size | 1060 bp |
RefSeq ORF | 945 bp |
Locus ID | 22040 |
UniProt ID | Q91XB0 |
MW | 33.7 kDa |
Gene Summary | Major cellular 3'-to-5' DNA exonuclease which digests single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) with mismatched 3' termini. Prevents cell-intrinsic initiation of autoimmunity. Acts by metabolizing DNA fragments from endogenous retroelements, including L1, LTR and SINE elements. Unless degraded, these DNA fragments accumulate in the cytosol and activate the IFN-stimulatory DNA (ISD) response and innate immune signaling. Prevents chronic ATM-dependent checkpoint activation, by processing ssDNA polynucleotide species arising from the processing of aberrant DNA replication intermediates. Inefficiently degrades oxidized DNA, such as that generated upon antimicrobial reactive oxygen production or upon absorption of UV light. During GZMA-mediated cell death, contributes to DNA damage in concert with NME1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC226976 | Trex1 (untagged) - Mouse three prime repair exonuclease 1 (Trex1), transcript variant 2 |
CNY 3,040.00 |