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Cyld (NM_001128170) Mouse Tagged ORF Clone
CAT＃: MR227175
- TrueORF®
Cyld (Myc-DDK-tagged) - Mouse cylindromatosis (turban tumor syndrome) (Cyld), transcript variant 3
ORF Plasmid: tGFP
"NM_001128170" in other vectors (3)
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CNY 9,500.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2010013M14Rik; 2900009M21Rik; C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227175 representing NM_001128170
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCAGGCCTGTGGAGCCAAGAGAAAGTTACTTCACCCTACTGGGAAGAACGGATTTTTTATCTGC TTCTTCAAGAATGCAGTGTAACAGACAAACAAACTCAGAAGCTGCTGAAAGTACCCAAAGGGAGTATAGG ACAGTACATCCAAGACCGTTCTGTGGGGCATTCAAGAGTTCCTTCCACAAAAGGCAAGAAAAATCAGATT GGATTAAAAATCTTGGAGCAACCGCATGCAGTTCTGTTTGTTGATGAAAAGGATGTTGTAGAAATAAATG AAAAATTCACAGAGTTACTGTTGGCAATTACCAACTGTGAGGAGAGGCTCAGCCTATTTAGAAACAGACT CCGACTAAGTAAAGGCCTCCAGGTAGACGTGGGCAGTCCTGTGAAAGTACAGCTGCGATCTGGGGAAGAG AAATTTCCAGGAGTTGTACGCTTCAGAGGACCTTTATTAGCGGAGAGGACGGTGTCGGGGATTTTCTTTG GAGTAGAATTATTGGAAGAAGGTCGTGGTCAAGGTTTCACGGATGGGGTATATCAAGGGAAGCAGCTTTT CCAGTGTGATGAGGACTGTGGCGTGTTTGTTGCATTGGACAAACTAGAACTTATAGAAGATGATGACAAT GGATTGGAAAGTGATTTTGCAGGCCCAGGAGATACAATGCAGGTTGAACCTCCCCCTTTGGAAATAAACT CCAGAGTTTCTTTGAAGGTTGGAGAAAGTACAGAATCTGGAACAGTAATATTCTGTGATGTTTTACCAGG AAAAGAGAGTCTAGGATATTTTGTTGGTGTGGACATGGATAACCCTATTGGCAACTGGGATGGAAGGTTT GATGGAGTACAGCTCTGTAGTTTTGCAAGTGTTGAAAGTACAATTCTCCTGCACATCAATGACATCATCC CAGCTTTATCAGATAGCGTGACACAGGAAAGGAGGCCTCCCAAACTTGCCTTTATGTCAAGAGGTGTAGG TGACAAAGGTTCATCTAGTCATAATAAACCAAAGGTTACAGGATCTACCTCAGACCCTGGAAGTAGAAAC AGATCTGAATTATTTTATACCTTAAATGGGTCATCTGTTGACTCACAACAATCCAAGTCCAAAAATCCAT GGTACATTGATGAAGTTGCAGAAGACCCTGCAAAGTCACTTACAGAGATGTCTTCGGACTTCGGACATTC ATCTCCTCCACCGCAGCCTCCTTCCATGAACTCCTTGTCTAGCGAGAACAGATTCCACTCCTTACCCTTC AGCCTGACAAAGATGCCCAATACTAATGGCAGCATGGCTCATAGTCCACTCTCTCTGTCAGTGCAGTCTG TGATGGGGGAGCTGAACAGCACACCTGTCCAGGAGAGTCCACCCTTGCCCATCTCTTCTGGGAATGCACA CGGGCTAGAGGTGGGCTCACTGGCTGAAGTAAAAGAGAACCCCCCGTTCTATGGGGTTATCCGTTGGATT GGCCAGCCACCAGGGCTCAGTGACGTGCTAGCTGGACTGGAACTGGAAGATGAATGCGCAGGCTGTACAG ATGGAACTTTCAGGGGCACGCGGTATTTCACGTGTGCCCTGAAGAAGGCACTGTTTGTGAAACTGAAGAG CTGCAGACCGGACTCTAGGTTTGCATCCTTGCAGCCTGTTTCCAATCAAATTGAAAGGTGTAACTCTTTA GCATTTGGGGGCTATTTAAGTGAAGTAGTAGAAGAAAATACTCCACCTAAAATGGAAAAGGAAGGTTTAG AGATAATGATTGGAAAGAAGAAAGGCATCCAGGGCCATTACAATTCTTGTTACTTAGACTCAACTTTATT CTGCTTATTTGCTTTTAGTTCTGCCCTGGACACTGTGTTACTTAGACCCAAAGAGAAGAATGATATAGAG TATTACAGTGAGACTCAGGAGCTACTGAGGACAGAGATAGTCAATCCTCTGAGAATATATGGATATGTGT GTGCCACAAAGATTATGAAACTGAGGAAAATACTTGAAAAAGTTGAGGCTGCATCAGGTTTTACCTCTGA AGAAAAAGATCCTGAAGAATTCTTAAATATCCTGTTTCATGATATTTTAAGGGTTGAACCATTGTTAAAA ATAAGATCAGCAGGTCAAAAAGTTCAAGACTGTAACTTCTATCAAATTTTTATGGAAAAAAATGAGAAAG TTGGAGTACCCACAATTCAGCAGTTATTAGAATGGTCTTTTATCAACAGCAACCTGAAATTTGCAGAGGC ACCATCATGCTTGATTATCCAGATGCCTCGGTTTGGAAAAGACTTTAAACTATTTAAAAAAATTTTTCCT TCCCTGGAATTAAATATAACAGATTTACTTGAAGACACTCCCAGGCAGTGCCGCATCTGTGGAGGACTTG CGATGTACGAGTGCAGGGAGTGCTATGACGATCCGGACATCTCAGCTGGGAAGATCAAGCAGTTCTGTAA GACCTGCAGCACTCAGGTTCACCTTCATCCCAGGAGGTTGAATCATTCTTATCATCCAGTATCACTTCCC AAAGACTTGCCTGACTGGGACTGGAGACACGGCTGCATCCCCTGTCAGAAGATGGAGTTATTTGCTGTTC TCTGCATAGAAACGAGCCACTATGTTGCTTTTGTGAAGTATGGGAAGGATGACTCTGCCTGGCTTTTCTT TGACAGCATGGCGGATCGAGATGGTGGTCAGAATGGCTTCAACATTCCACAAGTGACGCCCTGCCCAGAA GTGGGAGAGTACTTGAAGATGTCTCTGGAGGACCTGCACTCTTTGGATTCCAGAAGGATTCAAGGCTGTG CGCGCAGACTTCTTTGCGATGCATACATGTGCATGTACCAGAGTCCAACCATGAGCCTGTACAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227175 representing NM_001128170
Red=Cloning site Green=Tags(s) MSSGLWSQEKVTSPYWEERIFYLLLQECSVTDKQTQKLLKVPKGSIGQYIQDRSVGHSRVPSTKGKKNQI GLKILEQPHAVLFVDEKDVVEINEKFTELLLAITNCEERLSLFRNRLRLSKGLQVDVGSPVKVQLRSGEE KFPGVVRFRGPLLAERTVSGIFFGVELLEEGRGQGFTDGVYQGKQLFQCDEDCGVFVALDKLELIEDDDN GLESDFAGPGDTMQVEPPPLEINSRVSLKVGESTESGTVIFCDVLPGKESLGYFVGVDMDNPIGNWDGRF DGVQLCSFASVESTILLHINDIIPALSDSVTQERRPPKLAFMSRGVGDKGSSSHNKPKVTGSTSDPGSRN RSELFYTLNGSSVDSQQSKSKNPWYIDEVAEDPAKSLTEMSSDFGHSSPPPQPPSMNSLSSENRFHSLPF SLTKMPNTNGSMAHSPLSLSVQSVMGELNSTPVQESPPLPISSGNAHGLEVGSLAEVKENPPFYGVIRWI GQPPGLSDVLAGLELEDECAGCTDGTFRGTRYFTCALKKALFVKLKSCRPDSRFASLQPVSNQIERCNSL AFGGYLSEVVEENTPPKMEKEGLEIMIGKKKGIQGHYNSCYLDSTLFCLFAFSSALDTVLLRPKEKNDIE YYSETQELLRTEIVNPLRIYGYVCATKIMKLRKILEKVEAASGFTSEEKDPEEFLNILFHDILRVEPLLK IRSAGQKVQDCNFYQIFMEKNEKVGVPTIQQLLEWSFINSNLKFAEAPSCLIIQMPRFGKDFKLFKKIFP SLELNITDLLEDTPRQCRICGGLAMYECRECYDDPDISAGKIKQFCKTCSTQVHLHPRRLNHSYHPVSLP KDLPDWDWRHGCIPCQKMELFAVLCIETSHYVAFVKYGKDDSAWLFFDSMADRDGGQNGFNIPQVTPCPE VGEYLKMSLEDLHSLDSRRIQGCARRLLCDAYMCMYQSPTMSLYK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001128170 |
| ORF Size | 2865 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001128170.2 |
| RefSeq Size | 8022 bp |
| RefSeq ORF | 2868 bp |
| Locus ID | 74256 |
| UniProt ID | Q80TQ2 |
| MW | 107.3 kDa |
| Gene Summary | This gene encodes a protein that is a member of the ubiquitin C-terminal hydrolase subfamily of the deubiquitinating enzyme family. Members of this family catalyze the removal of ubiquitin from a substrate or another ubiquitin molecule and thereby play important roles in regulating signaling pathways, recycling ubiquitin and regulating protein stability. This protein removes ubiquitin from K-63-linked ubiquitin chains from proteins involved in NF-kappaB signaling and thus acts as a negative regulator of this pathway. In humans mutations in this gene have been associated with cylindromatosis, an autosomal dominant predisposition to tumors of skin appendages. In mouse deficiency of this gene impairs thymocyte development and increases susceptibility to skin and colon tumors. A pseudogene of this gene has been identified on chromosome 1. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227175 | Cyld (tGFP-tagged) - Mouse cylindromatosis (turban tumor syndrome) (Cyld) transcript variant 3, (10ug) |
CNY 10,450.00 |
|
| MR227175L3 | Lenti ORF clone of Cyld (Myc-DDK-tagged) - Mouse cylindromatosis (turban tumor syndrome) (Cyld), transcript variant 3 |
CNY 11,400.00 |
|
| MR227175L4 | Lenti ORF clone of Cyld (mGFP-tagged) - Mouse cylindromatosis (turban tumor syndrome) (Cyld), transcript variant 3 |
CNY 11,400.00 |
