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Map2k3 (NM_008928) Mouse Tagged ORF Clone
CAT＃: MR225898
- TrueORF®
Map2k3 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase 3 (Map2k3)
ORF Plasmid: tGFP
"NM_008928" in other vectors (4)
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CNY 3,420.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW212142; MEK3; MKK3; mMKK3b; Prkmk3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225898 representing NM_008928
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCGCCCGCCGCGAGCCCGCCGGCCAGCTTGCCTCAGACCAAAGGAAAATCCAAAAGGAAAAAGG ACTTACGGATATCCTGCGTGTCCAAGCCACCTGTGTCCAACCCCACACCCCCCCGGAACCTGGACTCCCG GACCTTCATCACTATCGGAGACAGAAACTTCGAAGTGGAGGCTGATGACTTGGTGACCATCTCAGAGCTG GGTCGTGGAGCCTATGGGGTGGTAGAGAAAGTGCGGCATGCTCAGAGTGGTACCATCATGGCTGTCAAGC GCATCCGGGCCACAGTGAACACACAGGAGCAGAAACGTCTGCTTATGGACCTAGACATCAACATGCGCAC GGTCGACTGCTTCTACACTGTCACCTTCTATGGTGCCCTCTTCAGAGAGGGGGATGTATGGATCTGCATG GAGCTCATGGACACTTCCCTGGATAAGTTCTACCGGAAGGTGCTGGAGAAGAACATGAAAATTCCGGAAG ACATCCTGGGGGAGATCGCTGTGTCTATCGTGCGGGCCCTGGAGCACCTGCATAGCAAGCTGTCTGTGAT CCACAGAGATGTGAAGCCATCCAATGTCCTCATCAACAAGGAAGGGCATGTGAAGATGTGCGACTTTGGC ATCAGTGGCTACCTGGTGGACTCTGTGGCAAAGACAATGGATGCTGGCTGCAAGCCTTACATGGCCCCTG AGAGGATCAACCCTGAACTGAATCAGAAGGGCTACAATGTCAAGTCTGATGTCTGGAGCCTCGGCATCAC CATGATCGAGATGGCCATTCTGCGATTCCCTTATGAGTCTTGGGGCACACCGTTCCAGCAGCTGAAGCAG GTGGTGGAGGAGCCATCCCCACAGCTCCCAGCGGACCAGTTCTCCCCTGAGTTTGTGGACTTCACTAGCC AGTGCCTAAGGAAGAACCCTGCAGAGCGCATGAGCTACCTGGAGCTGATGGAACACCCATTCTTCACCTT GCACAAAACTAAGAAGACAGACATTGCTGCCTTTGTGAAGGAGATCCTGGGAGAGGATTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225898 representing NM_008928
Red=Cloning site Green=Tags(s) MESPAASPPASLPQTKGKSKRKKDLRISCVSKPPVSNPTPPRNLDSRTFITIGDRNFEVEADDLVTISEL GRGAYGVVEKVRHAQSGTIMAVKRIRATVNTQEQKRLLMDLDINMRTVDCFYTVTFYGALFREGDVWICM ELMDTSLDKFYRKVLEKNMKIPEDILGEIAVSIVRALEHLHSKLSVIHRDVKPSNVLINKEGHVKMCDFG ISGYLVDSVAKTMDAGCKPYMAPERINPELNQKGYNVKSDVWSLGITMIEMAILRFPYESWGTPFQQLKQ VVEEPSPQLPADQFSPEFVDFTSQCLRKNPAERMSYLELMEHPFFTLHKTKKTDIAAFVKEILGEDS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008928 |
| ORF Size | 1041 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008928.5 |
| RefSeq Size | 2166 bp |
| RefSeq ORF | 1044 bp |
| Locus ID | 26397 |
| UniProt ID | O09110 |
| MW | 39.7 kDa |
| Gene Summary | Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209717 | Map2k3 (untagged) - Mouse mitogen-activated protein kinase kinase 3 (Map2k3), (10ug) |
CNY 3,656.00 |
|
| MG225898 | Map2k3 (tGFP-tagged) - Mouse mitogen-activated protein kinase kinase 3 (Map2k3), (10ug) |
CNY 3,800.00 |
|
| MR225898L3 | Lenti ORF clone of Map2k3 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase 3 (Map2k3) |
CNY 5,320.00 |
|
| MR225898L4 | Lenti ORF clone of Map2k3 (mGFP-tagged) - Mouse mitogen-activated protein kinase kinase 3 (Map2k3) |
CNY 5,320.00 |
