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Pde5a (NM_153422) Mouse Tagged ORF Clone
CAT＃: MR225179
- TrueORF®
Pde5a (Myc-DDK-tagged) - Mouse phosphodiesterase 5A, cGMP-specific (Pde5a)
ORF Plasmid: tGFP
"NM_153422" in other vectors (4)
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CNY 6,336.00
CNY 7,130.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Cgbpde; Cn5n; Pde5; Pde5a1; PDE5a2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225179 representing NM_153422
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACGAGCGGGCCCCAACTCCGTGCGGTCGCAGCAGCAGCGGGACCCGGACTGGGTGGAAGCGTGGC TGGATGATCACCGGGACTTTACCTTCTCTTACTTTATTAGAAAGGCCACCAGAGACATGGTCAACGCATG GTTTTCAGAGAGAGTTCACAACATCCCTGTGTGCAAGGAAGGCATCCGAGCCCATACCGAGTCCTGCTCT TGCTCTCTGCAGCAAAGTCCTCATGCAGATAATACCACCCCTGGAGCACCAGCCAGAAAAATATCTGCCT CTGAATTTGACCGACCTCTTAGACCCATTGTTGTCAAGGATTCCGAGGGAACAGTGAGCTTTCTCTCTGA CTCAGGAAAAAAGGAACAAATGCCACTAACCCCCCCAAGATTTGATAGTGATGAAGGGGACCAGTGCTCA AGGCTCTTGGAGCTAGTAAAAGATATTTCCAGTCATTTGGATGTCACAGCATTGTGTCACAAAATTTTCT TGCATATCCATGGACTCATCTCTGCGGATCGCTATTCCCTGTTCCTTGTCTGTGAGGACAGCTCTAAAGA CAAATTCCTCATCAGCCGCCTCTTTGATGTTGCTGAAGGTTCAACATTGGAGGAAGCTTCAAATAACTGT ATCCGTTTAGAGTGGAACAAAGGCATTGTGGGACATGTGGCAGCTTTTGGCGAGCCCTTGAACATCAAAG ATGCCTATGAGGATCCCCGGTTCAATGCAGAAGTTGACCAAATTACAGGCTACAAGACACAAAGTATCCT TTGTATGCCAATTAAGAACCACAGGGAAGAGGTTGTTGGTGTAGCTCAGGCCATCAACAAGAAATCAGGA AATGGTGGGACCTTCACTGAAAAAGATGAAAAGGACTTTGCGGCCTACCTGGCATTCTGTGGTATTGTTC TTCACAATGCTCAGCTCTATGAAACGTCATTGCTGGAGAACAAAAGAAATCAGGTGTTACTTGACCTTGC CAGCTTAATCTTTGAAGAGCAGCAGTCACTGGAAGTCATTCTGAAGAAGATAGCGGCCACTATCATCTCC TTCATGCAGGTGCAGAAGTGCACCATCTTCATTGTGGATGAAGACTGTCCTGACTCTTTCTCTCGTGTGT TTCACATGGAGTGTGAGGAAGTAGGAAAACCCTCTGATCCTTTAACGAGGGAGCAAGATGCAAACAAAAT CAACTACATGTATGCTCAGTATGTCAAAAACACGATGGAGCCACTTAATATCCCAGATGTCACCAAGGAC AAAAGATTTCCCTGGACAAATGAAAACATGGGACACGTGAACACGCCCTGCATTGGAAGTTTGCTTTGCA CCCCTATAAAAAATGGGAAGAAGAACAAAGTCATAGGCGTCTGCCAACTTGTTAATAAGATGGAGGAGAA TACTGGCAAGATTAAAGCTTTCAACCAAAATGATGAGCAGTTCCTGGAAGCCTTTGTCATCTTCTGTGGC TTGGGGATCCAGAACACACAGATGTATGAAGCGGTGGAGAGAGCCATGGCCAAGCAGATGGTGACATTAG AGGTCCTGTCTTACCATGCATCAGCGGCGGAGGAAGAAACAAGAGAGCTCCAGGCTTTATCGGCTGCTGT GGTACCATCGGCCCAAACCCTTAAAATTACCGACTTCAGCTTCAGTGACTTTGAGCTGTCTGATCTGGAA ACAGCGCTGTGTACAATTCGGATGTTCACTGACCTCAACCTTGTGCAGAACTTCCAGATGAAACATGAGG TTCTTTGCAGGTGGATTTTAAGTGTGAAGAAGAATTATCGGAAGAACGTTGCCTACCACAATTGGAGGCA TGCCTTTAACACGGCACAGTGCATGTTTGCTGCTCTGAAAGCCGGCAAGATCCAGAACAAGTTGACGGAT CTGGAGACCCTTGCGTTGCTCATTGCTGCTTTAAGCCATGACTTGGATCATCGTGGCGTGAACAACTCAT ACATACAGCGGAGCGAGCACCCGCTGGCTCAGCTGTACTGTCACTCCATCATGGAACACCATCATTTTGA CCAGTGCTTGATGATTCTAAACAGCCCAGGCAACCAGATTCTCAGTGGCCTCTCCATTGACGAATATAAG ACCACACTGAAAATAATCAAGCAAGCAATTTTAGCCACAGACCTAGCACTGTACATTAAGAGACGAGGAG AATTTTTTGAACTTATACGAAAAAATCAATTCAGTTTTGAAGATCCTCTTCAAAAGGAGTTGTTTCTAGC AATGCTGATGACAGCCTGCGACCTCTCTGCGATTACTAAACCCTGGCCTATTCAACAACGGATAGCAGAA CTCGTGGCAGCTGAATTCTTTGATCAAGGAGACAGAGAGAGAAAAGAACTTAACATGGAGCCGGCTGATC TAATGAACAGAGAGAAGAAAAATAAAATCCCAAGTATGCAAGTTGGGTTCATAGATGCCATCTGCTTGCA GCTGTATGAGGCCCTGACCCACGTATCCGAAGACTGTTTGCCCTTGCTGGATGGCTGCAGGAAGAACAGA CAGAAATGGCAGGCCCTCGCGGAGCAGCAGGAGAAGATGCTCCTTAACGGGGAGAGCAGCCAGGGCAAGC GGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225179 representing NM_153422
Red=Cloning site Green=Tags(s) MERAGPNSVRSQQQRDPDWVEAWLDDHRDFTFSYFIRKATRDMVNAWFSERVHNIPVCKEGIRAHTESCS CSLQQSPHADNTTPGAPARKISASEFDRPLRPIVVKDSEGTVSFLSDSGKKEQMPLTPPRFDSDEGDQCS RLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSKDKFLISRLFDVAEGSTLEEASNNC IRLEWNKGIVGHVAAFGEPLNIKDAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSG NGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIIS FMQVQKCTIFIVDEDCPDSFSRVFHMECEEVGKPSDPLTREQDANKINYMYAQYVKNTMEPLNIPDVTKD KRFPWTNENMGHVNTPCIGSLLCTPIKNGKKNKVIGVCQLVNKMEENTGKIKAFNQNDEQFLEAFVIFCG LGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETRELQALSAAVVPSAQTLKITDFSFSDFELSDLE TALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTD LETLALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIDEYK TTLKIIKQAILATDLALYIKRRGEFFELIRKNQFSFEDPLQKELFLAMLMTACDLSAITKPWPIQQRIAE LVAAEFFDQGDRERKELNMEPADLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCLPLLDGCRKNR QKWQALAEQQEKMLLNGESSQGKRD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153422 |
| ORF Size | 2595 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153422.2, NP_700471.2 |
| RefSeq Size | 6728 bp |
| RefSeq ORF | 2598 bp |
| Locus ID | 242202 |
| UniProt ID | Q8CG03 |
| MW | 98.9 kDa |
| Gene Summary | Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. Specifically regulates nitric-oxide-generated cGMP.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Myoglobin maturation is driven by the hsp90 chaperone machinery and by soluble guanylyl cyclase
,Ghosh, A;Dai, Y;Biswas, P;Stuehr, DJ;,
FASEB J.
,PubMed ID 31170354
[PDE5A]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222287 | Pde5a (untagged) - Mouse phosphodiesterase 5A, cGMP-specific (Pde5a), (10ug) |
CNY 11,120.00 |
|
| MG225179 | Pde5a (tGFP-tagged) - Mouse phosphodiesterase 5A cGMP-specific (Pde5a), (10ug) |
CNY 7,890.00 |
|
| MR225179L3 | Lenti ORF clone of Pde5a (Myc-DDK-tagged) - Mouse phosphodiesterase 5A, cGMP-specific (Pde5a) |
CNY 9,030.00 |
|
| MR225179L4 | Lenti ORF clone of Pde5a (mGFP-tagged) - Mouse phosphodiesterase 5A, cGMP-specific (Pde5a) |
CNY 9,030.00 |
