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Cnn1 (NM_009922) Mouse Tagged ORF Clone
CAT＃: MR219445
- TrueORF®
Cnn1 (Myc-DDK-tagged) - Mouse calponin 1 (Cnn1)
ORF Plasmid: tGFP
"NM_009922" in other vectors (4)
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CNY 2,081.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CN; CnnI |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR219445 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTCTGCACATTTTAACCGAGGTCCTGCCTACGGCTTGTCTGCTGAAGTAAAGAACAAGCTGGCCC AGAAATACGACCATCAGCGGGAGCAGGAGCTGAGAGAGTGGATTGAGGGGGTGACAGGCCGGCGCATCGG GAACAACTTCATGGATGGCCTCAAAGATGGGATCATTCTTTGCGAATTTATCAACAAGCTGCAGCCGGGT TCTGTGAAGAAGGTCAATGAGTCAACTCAGAACTGGCACCAGCTGGAGAACATAGGTAATTTCATCAAAG CCATTACCAAGTATGGGGTGAAACCCCACGACATCTTTGAGGCCAACGACCTGTTTGAAAACACCAACCA TACACAAGTTCAGTCCACTCTCCTGGCTCTGGCCAGCATGGCCAAGACAAAAGGAAACAAAGTCAATGTG GGAGTCAAGTATGCAGAGAAACAAGAGCGGAGATTTGAGCCGGAGAAGTTGAGAGAAGGCAGGAACATCA TTGGACTGCAGATGGGCACCAACAAGTTTGCCAGTCAGCAGGGCATGACGGCCTATGGTACACGGCGTCA CCTCTATGATCCCAAACTGGGTACAGATCAGCCTCTGGACCAGGCGACCATCAGCCTGCAGATGGGCACC AACAAGGGTGCCAGCCAGGCTGGCATGACTGCACCAGGCACCAAGCGGCAGATCTTTGAGCCAGGTCTGG GCATGGAACACTGCGACACACTCAACGTCAGCTTGCAGATGGGCAGCAACAAGGGGGCCTCCCAGAGGGG CATGACAGTGTATGGGCTTCCTCGCCAGGTGTACGATCCCAAGTACTGCCTGAACCCGGAGTACCCAGAG CTGAGTGAGCCCACCCACAATCACCACCCGCACAACTACTACAACTCTGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR219445 protein sequence
Red=Cloning site Green=Tags(s) MSSAHFNRGPAYGLSAEVKNKLAQKYDHQREQELREWIEGVTGRRIGNNFMDGLKDGIILCEFINKLQPG SVKKVNESTQNWHQLENIGNFIKAITKYGVKPHDIFEANDLFENTNHTQVQSTLLALASMAKTKGNKVNV GVKYAEKQERRFEPEKLREGRNIIGLQMGTNKFASQQGMTAYGTRRHLYDPKLGTDQPLDQATISLQMGT NKGASQAGMTAPGTKRQIFEPGLGMEHCDTLNVSLQMGSNKGASQRGMTVYGLPRQVYDPKYCLNPEYPE LSEPTHNHHPHNYYNSA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009922 |
| ORF Size | 894 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009922.1, NM_009922.2, NM_009922.3, NM_009922.4, NP_034052.3 |
| RefSeq Size | 1987 bp |
| RefSeq ORF | 894 bp |
| Locus ID | 12797 |
| UniProt ID | Q08091 |
| MW | 33.4 kDa |
| Gene Summary | Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Inhibiting Monoacylglycerol Lipase Suppresses RANKL-Induced Osteoclastogenesis and Alleviates Ovariectomy-Induced Bone Loss
,Liu, H;Zhou, C;Qi, D;Gao, Y;Zhu, M;Tao, T;Sun, X;Xiao, J;,
Frontiers in cell and developmental biology
,PubMed ID 33777947
[CNN1]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208305 | Cnn1 (untagged) - Mouse calponin 1 (Cnn1), (10ug) |
CNY 3,990.00 |
|
| MG219445 | Cnn1 (tGFP-tagged) - Mouse calponin 1 (Cnn1), (10ug) |
CNY 2,850.00 |
|
| MR219445L3 | Lenti ORF clone of Cnn1 (Myc-DDK-tagged) - Mouse calponin 1 (Cnn1) |
CNY 4,750.00 |
|
| MR219445L4 | Lenti ORF clone of Cnn1 (mGFP-tagged) - Mouse calponin 1 (Cnn1) |
CNY 4,750.00 |
