Acaca (NM_133360) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	A530025K05Rik; Acac; Acc1; Gm738
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR216287 representing NM_133360 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGAACCATCTCCGTTGGCCAAAACTCTGGAGCTAAACCAGCACTCCCGATTCATAATTGGGTCTG TGTCTGAAGACAACTCAGAAGATGAGATCAGTAACCTGGTGAAGCTGGACCTAGAAGAGAAGGAGGGCTC CCTGTCACCAGCCTCCGTCAGCTCAGATACACTTTCTGATTTGGGGATCTCTGGCTTACAGGATGGTTTG GCCTTTCACATGAGATCCAGCATGTCTGGCTTGCACCTAGTAAAACAAGGTCGAGACAGAAAGAAAATAG ACTCACAACGAGATTTCACTGTGGCTTCTCCAGCAGAATTTGTTACTCGTTTTGGGGGAAATAAAGTAAT TGAGAAGGTTCTTATCGCCAACAATGGTATTGCAGCAGTGAAATGCATGCGATCTATCCGTCGGTGGTCT TATGAAATGTTTCGAAATGAACGTGCAATCCGATTTGTTGTCATGGTTACACCTGAAGACCTTAAAGCCA ATGCAGAATACATTAAGATGGCAGACCACTATGTTCCAGTGCCTGGAGGACCCAACAACAACAATTACGC AAATGTGGAGTTGATTCTTGATATTGCTAAAAGGATACCTGTACAAGCAGTGTGGGCTGGCTGGGGTCAT GCCTCTGAGAACCCGAAACTCCCAGAACTGCTCTTAAAAAATGGCATTGCTTTCATGGGCCCTCCAAGCC AGGCCATGTGGGCTTTGGGGGATAAGATTGCATCTTCTATTGTGGCTCAAACTGCAGGTATCCCAACTCT TCCCTGGAGTGGCAGTGGTCTTCGAGTGGATTGGCAAGAAAATGATTTTTCGAAACGTATCTTAAATGTT CCACAGGATCTGTATGAGAAAGGCTATGTGAAGGATGTGGATGATGGTCTGAAGGCAGCTGAGGAAGTTG GCTATCCAGTGATGATCAAGGCCTCAGAGGGAGGAGGAGGGAAAGGGATCAGAAAAGTTAACAATGCAGA TGACTTCCCTAACCTCTTCAGACAGGTTCAAGCTGAAGTTCCTGGATCACCTATATTTGTAATGAGACTA GCAAAACAATCTCGACATCTGGAGGTCCAGATTCTGGCAGATCAGTATGGCAATGCTATTTCTTTGTTTG GTCGTGACTGCTCTGTGCAGCGCAGGCATCAGAAGATCATTGAAGAAGCTCCTGCTGCGATTGCTACCCC AGCAGTATTTGAACACATGGAACAGTGTGCAGTGAAACTTGCCAAAATGGTTGGTTATGTGAGTGCTGGG ACTGTGGAATACTTGTACAGCCAGGATGGAAGCTTCTACTTTTTGGAACTGAACCCTCGGCTACAGGTTG AACATCCTTGTACAGAGATGGTGGCTGATGTCAATCTTCCTGCAGCACAGCTCCAGATTGCCATGGGGAT CCCTCTATTTAGGATCAAGGATATTCGTATGATGTATGGGGTATCTCCTTGGGGAGATGCTCCCATTGAT TTTGAAAATTCTGCTCATGTTCCTTGCCCAAGGGGCCACGTGATTGCTGCTCGGATCACCAGTGAAAACC CAGATGAGGGGTTTAAGCCCAGCTCTGGAACAGTTCAGGAACTTAATTTTCGTAGCAATAAGAACGTTTG GGGTTATTTCAGTGTTGCTGCTGCTGGAGGACTTCATGAATTTGCTGATTCTCAGTTCGGGCACTGCTTT TCCTGGGGAGAAAACAGGGAGGAAGCAATCTCAAATATGGTGGTGGCACTGAAGGAGCTGTCTATTCGGG GTGACTTTCGAACTACAGTGGAATACCTCATCAAACTGCTGGAGACAGAAAGCTTTCAGCTTAACAGAAT CGACACTGGCTGGCTGGACAGACTGATCGCAGAGAAAGTGCAGGCAGAGCGACCTGACACCATGTTGGGA GTTGTGTGTGGGGCTCTCCATGTAGCAGATGTGAGCCTGAGGAACAGCATCTCTAACTTCCTTCACTCCT TAGAGAGGGGTCAAGTCCTTCCTGCTCACACACTTCTGAACACAGTAGATGTTGAACTTATCTATGAAGG AATCAAATATGTACTTAAGGTGACTCGGCAGTCTCCCAACTCCTACGTAGTGATAATGAATGGCTCGTGT GTGGAAGTGGATGTGCATCGGCTGAGTGATGGTGGCCTGCTCTTGTCTTATGACGGCAGCAGTTACACCA CATACATGAAGGAAGAGGTGGACAGATATCGAATCACAATTGGCAATAAAACCTGTGTGTTTGAGAAGGA AAATGACCCATCTGTAATGCGCTCACCGTCTGCTGGGAAGTTAATCCAGTATATTGTGGAAGATGGCGGA CATGTGTTTGCTGGCCAGTGCTATGCTGAGATTGAGGTAATGAAGATGGTGATGACTTTAACAGCTGTAG AATCTGGCTGCATCCATTATGTCAAACGACCTGGAGCAGCACTTGACCCTGGCTGTGTGATAGCCAAAAT GCAACTGGACAACCCCAGTAAAGTTCAACAGGCTGAGCTTCACACGGGCAGTCTACCACAGATCCAGAGC ACAGCTCTCAGAGGCGAGAAGCTCCATCGAGTTTTCCACTATGTCCTGGATAACCTGGTCAATGTGATGA ATGGATACTGCCTTCCAGACCCTTTCTTCAGCAGCAGGGTAAAAGACTGGGTAGAAAGATTGATGAAGAC TCTGAGAGACCCCTCCTTGCCTCTGCTAGAGCTGCAGGATATCATGACCAGTGTCTCTGGCCGGATCCCC CTCAATGTGGAGAAGTCTATTAAGAAGGAAATGGCTCAGTATGCTAGCAACATCACATCAGTCCTGTGTC AGTTTCCCAGCCAGCAGATTGCCAACATCCTAGATAGTCATGCAGCTACACTGAACCGGAAATCTGAGCG GGAAGTCTTCTTCATGAACACCCAGAGCATTGTCCAGCTGGTGCAGAGGTACCGAAGTGGCATCCGTGGC CACATGAAGGCTGTGGTGATGGATCTGCTGCGGCAGTACCTGCGAGTAGAGACACAGTTTCAGAACGGCC ACTACGACAAATGTGTATTCGCCCTTCGGGAAGAGAACAAAAGCGACATGAACACCGTACTGAACTACAT CTTCTCCCACGCTCAGGTCACCAAAAAGAATCTCCTGGTGACAATGCTTATTGATCAGTTATGTGGCCGG GACCCTACACTTACTGATGAGCTGCTAAATATCCTCACAGAGCTAACCCAACTCAGCAAGACCACCAACG CTAAAGTGGCGCTGCGCGCTCGTCAGGTTCTTATTGCTTCCCATTTGCCATCATATGAGCTTCGCCATAA CCAAGTAGAGTCTATCTTCCTATCAGCCATTGACATGTATGGACACCAGTTTTGCATTGAGAACCTGCAG AAACTCATCCTCTCGGAAACATCTATTTTCGATGTCCTCCCAAACTTTTTTTACCACAGCAACCAGGTGG TGAGGATGGCAGCTCTGGAGGTGTATGTTCGAAGGGCTTACATTGCCTATGAACTCAACAGCGTACAACA CCGCCAGCTTAAGGACAACACCTGTGTGGTGGAATTTCAGTTCATGCTGCCCACATCCCATCCAAACAGA GGGAACATCCCCACGCTAAACAGAATGTCCTTTGCCTCCAACCTCAACCACTATGGCATGACTCATGTAG CTAGTGTCAGCGATGTTCTGTTGGACAACGCCTTCACGCCACCTTGTCAGCGGATGGGCGGAATGGTCTC TTTCCGGACCTTTGAAGATTTTGTCAGGATCTTTGATGAAATAATGGGCTGCTTCTGTGACTCCCCACCC CAAAGCCCCACATTCCCAGAGTCTGGTCATACTTCGCTCTATGATGAAGACAAGGTCCCCAGGGATGAAC CAATACATATTCTGAATGTGGCTATCAAGACTGATGGCGATATTGAGGATGACAGGCTTGCAGCTATGTT CAGAGAGTTCACCCAGCAGAATAAAGCTACTTTGGTTGAGCATGGCATCCGGCGACTTACGTTCCTAGTT GCACAAAAGGATTTCAGAAAACAAGTCAACTGTGAGGTGGATCAGAGATTTCATAGAGAATTCCCCAAAT TTTTCACATTCCGAGCAAGGGATAAGTTTGAGGAGGACCGCATTTATCGACACCTGGAGCCTGCTCTGGC TTTCCAGTTAGAGTTGAACCGGATGAGAAATTTTGACCTTACTGCCATCCCATGTGCTAATCACAAGATG CACCTGTACCTTGGGGCTGCTAAGGTGGAAGTAGGCACAGAAGTGACTGACTACAGGTTCTTTGTTCGTG CGATCATCAGGCACTCTGATCTGGTCACAAAGGAAGCTTCTTTCGAATATCTACAAAATGAAGGGGAACG ACTGCTCCTGGAAGCTATGGATGAATTGGAAGTTGCTTTTAATAATACAAATGTCCGCACTGACTGTAAC CACATCTTCCTCAACTTTGTGCCCACGGTCATCATGGACCCATCAAAGATTGAAGAATCTGTGCGGAGCA TGGTAATGCGCTATGGAAGTCGGCTATGGAAATTGCGGGTCCTCCAGGCAGAACTGAAAATCAACATTCG CCTGACAACAACTGGAAAAGCAATTCCCATCCGCCTCTTCCTGACAAACGAGTCTGGCTACTACTTGGAC ATCAGCCTGTATAAGGAAGTGACTGACTCCAGGACAGCACAGATCATGTTTCAGGCATATGGAGACAAGC AGGGACCACTGCATGGAATGTTAATTAATACTCCATATGTGACCAAAGACCTTCTTCAATCAAAGAGGTT CCAGGCACAGTCCTTAGGAACAACATATATATATGATATCCCAGAGATGTTTCGGCAGTCACTCATCAAA CTCTGGGAGTCCATGTCCACCCAAGCATTTCTTCCTTCGCCTCCTTTGCCTTCCGACATCCTGACGTATA CTGAACTGGTGTTGGATGATCAAGGCCAGCTGGTCCATATGAACAGACTTCCAGGAGGAAATGAGATTGG CATGGTAGCCTGGAAAATGAGCCTTAAAAGCCCTGAATATCCAGATGGCCGAGATATCATTGTCATCGGC AATGACATTACATATCGGATCGGTTCCTTTGGGCCTCAGGAGGATTTGCTGTTTCTCAGAGCTTCTGAAC TTGCCAGAGCAGAAGGCATCCCACGCATCTACGTGGCAGCGAACAGTGGAGCTAGAATTGGACTTGCAGA AGAAATACGCCATATGTTCCATGTGGCCTGGGTAGATCCTGAAGATCCCTACAAGGGATACAAGTATTTA TATCTGACACCCCAGGATTATAAGAGAGTCAGTGCCCTCAATTCTGTCCACTGTGAACATGTGGAGGATG AAGGGGAATCCAGGTACAAGATAACAGATATTATCGGGAAAGAAGAAGGACTTGGAGCAGAGAACCTTCG GGGTTCTGGAATGATTGCTGGGGAATCCTCATTGGCTTACGATGAGGTCATCACCATCAGCCTGGTTACA TGCCGGGCCATTGGTATTGGGGCTTACCTTGTCCGGCTGGGACAAAGAACCATCCAGGTTGAGAATTCTC ACTTAATTCTGACAGGAGCAGGTGCCCTCAACAAAGTCCTTGGTCGGGAAGTATACACCTCCAACAACCA GCTTGGGGGCATCCAGATTATGCACAACAATGGGGTTACCCACTCCACTGTTTGTGATGACTTTGAGGGA GTCTTCACAGTCTTACACTGGCTGTCATACATGCCGAAGAGCGTACACAGTTCAGTTCCTCTCCTGAATT CCAAGGATCCTATAGATAGAATCATCGAGTTTGTTCCCACAAAGGCCCCATATGATCCTCGGTGGATGCT AGCAGGCCGTCCTCACCCAACCCAGAAAGGCCAATGGTTGAGTGGGTTTTTTGACTATGGATCTTTCTCA GAAATCATGCAGCCCTGGGCACAGACCGTGGTAGTTGGCAGAGCCAGGTTAGGGGGAATACCCGTGGGAG TAGTTGCTGTAGAAACCCGAACAGTGGAACTCAGTATCCCAGCTGATCCTGCGAACCTGGATTCTGAAGC CAAGATAATCCAGCAGGCCGGCCAGGTTTGGTTCCCAGACTCTGCATTTAAGACCTATCAAGCTATCAAG GACTTTAACCGTGAAGGGCTACCTCTAATGGTCTTTGCCAACTGGAGAGGTTTCTCTGGTGGGATGAAAG ATATGTATGACCAAGTGCTCAAGTTTGGCGCTTACATTGTGGATGGCTTGCGGGAATGTTCCCAGCCTGT AATGGTTTACATCCCGCCCCAGGCTGAGCTTCGGGGTGGTTCTTGGGTTGTGATCGACCCCACCATCAAC CCTCGGCACATGGAGATGTACGCTGACCGAGAAAGCAGGGGATCTGTTCTGGAGCCAGAAGGGACAGTAG AAATCAAATTCCGTAAAAAGGATCTGGTGAAAACCATGCGTCGGGTAGATCCAGTTTACATCCGCTTGGC TGAGCGATTGGGCACCCCAGAGCTAAGCCCCACTGAGCGGAAGGAGCTGGAGAGCAAGTTGAAGGAGCGG GAGGAGTTCCTAATTCCCATTTACCATCAGGTAGCTGTGCAGTTTGCTGACTTGCACGACACACCAGGCC GGATGCAGGAGAAGGGTGTCATTAACGATATCTTGGATTGGAAAACATCCCGCACCTTCTTCTACTGGAG GCTGAGGCGCCTCCTGCTGGAGGACCTGGTCAAGAAGAAAATCCACAATGCCAACCCTGAGCTGACTGAC GGCCAGATCCAGGCCATGTTGAGACGCTGGTTTGTAGAAGTTGAAGGCACAGTGAAGGCTTACGTCTGGG ACAATAATAAGGACCTGGTGGAGTGGCTGGAGAAGCAACTGACAGAGGAAGATGGCGTCCGCTCTGTGAT AGAGGAGAACATCAAATACATCAGCAGGGACTATGTCCTGAAGCAGATCCGCAGCTTGGTCCAGGCCAAC CCAGAAGTCGCCATGGACTCCATCGTCCACATGACCCAGCACATCTCACCCACCCAGCGAGCAGAAGTTG TAAGGATCCTCTCCACTATGGATTCCCCTTCCACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR216287 representing NM_133360 Red=Cloning site Green=Tags(s) MDEPSPLAKTLELNQHSRFIIGSVSEDNSEDEISNLVKLDLEEKEGSLSPASVSSDTLSDLGISGLQDGL AFHMRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWS YEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGH ASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWQENDFSKRILNV PQDLYEKGYVKDVDDGLKAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRL AKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPAAIATPAVFEHMEQCAVKLAKMVGYVSAG TVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLFRIKDIRMMYGVSPWGDAPID FENSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCF SWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQLNRIDTGWLDRLIAEKVQAERPDTMLG VVCGALHVADVSLRNSISNFLHSLERGQVLPAHTLLNTVDVELIYEGIKYVLKVTRQSPNSYVVIMNGSC VEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVMRSPSAGKLIQYIVEDGG HVFAGQCYAEIEVMKMVMTLTAVESGCIHYVKRPGAALDPGCVIAKMQLDNPSKVQQAELHTGSLPQIQS TALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSRVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIP LNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRG HMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGR DPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQ KLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNR GNIPTLNRMSFASNLNHYGMTHVASVSDVLLDNAFTPPCQRMGGMVSFRTFEDFVRIFDEIMGCFCDSPP QSPTFPESGHTSLYDEDKVPRDEPIHILNVAIKTDGDIEDDRLAAMFREFTQQNKATLVEHGIRRLTFLV AQKDFRKQVNCEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKM HLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCN HIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTTTGKAIPIRLFLTNESGYYLD ISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIK LWESMSTQAFLPSPPLPSDILTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMSLKSPEYPDGRDIIVIG NDITYRIGSFGPQEDLLFLRASELARAEGIPRIYVAANSGARIGLAEEIRHMFHVAWVDPEDPYKGYKYL YLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGAENLRGSGMIAGESSLAYDEVITISLVT CRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHSTVCDDFEG VFTVLHWLSYMPKSVHSSVPLLNSKDPIDRIIEFVPTKAPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFS EIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIK DFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDGLRECSQPVMVYIPPQAELRGGSWVVIDPTIN PRHMEMYADRESRGSVLEPEGTVEIKFRKKDLVKTMRRVDPVYIRLAERLGTPELSPTERKELESKLKER EEFLIPIYHQVAVQFADLHDTPGRMQEKGVINDILDWKTSRTFFYWRLRRLLLEDLVKKKIHNANPELTD GQIQAMLRRWFVEVEGTVKAYVWDNNKDLVEWLEKQLTEEDGVRSVIEENIKYISRDYVLKQIRSLVQAN PEVAMDSIVHMTQHISPTQRAEVVRILSTMDSPST TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_133360
ORF Size	7035 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_133360.2, NP_579938.2
RefSeq Size	9054 bp
RefSeq ORF	7038 bp
Locus ID	107476
UniProt ID	Q5SWU9
MW	265.7 kDa
Gene Summary	Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.[UniProtKB/Swiss-Prot Function]

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