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Mark4 (NM_172279) Mouse Tagged ORF Clone
CAT＃: MR215461
- TrueORF®
Mark4 (Myc-DDK-tagged) - Mouse MAP/microtubule affinity-regulating kinase 4 (Mark4)
ORF Plasmid: tGFP
"NM_172279" in other vectors (4)
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CNY 5,512.00
CNY 6,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2410090P21Rik; C79806; Markl1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR215461 representing NM_172279
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTCGAGGACGGCGCTGGCCCCGGGCAACGATCGAAACTCGGACACGCATGGCACATTGGGCAGTG GACGATCTTCGGACAAAGGGCCGTCCTGGTCCAGCCGTTCCCTGGGTGCCCGTTGCCGGAACTCTATCGC TTCCTGCCCTGAGGAACAACCCCATGTGGGCAACTATAGGCTGCTAAGGACCATCGGGAAGGGCAACTTC GCCAAAGTCAAGCTGGCTCGGCATATCCTCACGGGCCGGGAGGTCGCTATTAAGATCATTGATAAGACCC AGCTGAACCCCAGTAGCTTGCAGAAGCTGTTCAGAGAAGTCCGAATTATGAAGGGACTCAACCACCCCAA CATCGTGAAGCTTTTTGAGGTGATAGAGACGGAGAAGACGCTATACCTGGTGATGGAATACGCTAGCGCA GGAGAAGTGTTTGACTACCTCGTGTCGCACGGCCGCATGAAGGAGAAGGAGGCTCGAGCCAAGTTCCGGC AGATCGTGTCAGCCGTGCACTACTGTCATCAGAAGAACATTGTACACAGGGATCTAAAGGCTGAAAACCT GTTGCTGGATGCCGAGGCCAACATCAAAATCGCCGACTTCGGCTTCAGCAATGAGTTCACGCTGGGCTCC AAGCTGGACACCTTCTGTGGGAGCCCCCCATACGCCGCCCCAGAGCTGTTCCAGGGCAAGAAGTATGATG GGCCAGAGGTGGACATCTGGAGCCTGGGTGTCATCCTGTACACGCTGGTCAGCGGCTCCCTGCCCTTCGA TGGGCACAACCTCAAGGAGCTGCGGGAGCGAGTCCTCAGAGGAAAGTACCGGGTCCCCTTCTACATGTCT ACAGACTGCGAGAGCATTCTGCGGAGATTTCTGGTGCTGAACCCCGCAAAACGCTGTACTCTGGAGCAAA TCATGAAAGACAAATGGATCAACATCGGCTATGAGGGTGAGGAGCTGAAGCCATACACGGAGCCTGAGGA GGACTTCGGGGACACCAAGAGAATTGAGGTGATGGTGGGTATGGGCTACACACGGGAAGAAATCAAAGAG GCCTTGACCAACCAGAAGTACAACGAGGTGACCGCCACCTACCTCCTGCTGGGCAGGAAGACTGAGGAGG GTGGGGACCGGGGTGCCCCAGGGCTGGCCCTGGCACGGGTGCGGGCGCCCAGCGACACCACCAACGGGAC AAGCTCCAGCAAAGGCAGCAGCCACAACAAAGGGCAACGGGCTTCTTCCTCCACCTACCACCGCCAGCGC CGTCACAGTGACTTCTGTGGCCCGTCCCCTGCCCCGCTGCACCCGAAGCGCAGCCCAACCAGCACGGGAG ACACGGAGCTCAAAGAAGAGCGGATGCCGGGTCGGAAAGCGAGCTGCAGTGCAGTGGGCAGTGGAAGTCG AGGCTTGCCCCCCTCCAGCCCCATGGTCAGCAGTGCCCACAACCCCAATAAGGCAGAGATCCCTGAGCGG CGGAAGGACAGCACTAGCACCCCTAACAACCTCCCCCCCAGCATGATGACCCGAAGAAACACCTATGTGT GCACAGAGCGACCAGGATCTGAACGCCCGTCCTTGTTGCCAAATGGCAAAGAAAATAGCTCCGGTACCTC GCGGGTGCCCCCTGCCTCGCCTTCCAGTCATAGCCTGGCTCCCCCGTCAGGCGAGCGGAGCCGCCTGGCT CGGGGCTCCACCATCCGCAGCACCTTCCATGGGGGCCAGGTCCGAGACCGGCGGGCAGGGAGCGGGAGTG GCGGGGGTGTGCAGAATGGACCCCCAGCCTCACCCACGCTTGCCCACGAGGCCGCACCCCTGCCCTCCGG GCGGCCTCGCCCCACCACCAACCTCTTCACCAAGCTGACCTCCAAACTGACCCGAAGGGTCACAGACGAA CCTGAGAGAATCGGGGGACCTGAGGTCACAAGTTGCCATCTACCTTGGGATAAAACGGAAACCGCCCCCA GGCTGCTCCGATTCCCCTGGAGTGTGAAGCTGACCAGCTCGCGACCTCCTGAGGCCCTGATGGCTGCCCT GCGACAGGCCACAGCGGCCGCCCGCTGCCGGTGCCGCCAGCCGCAGCCGTTCCTGCTGGCCTGCCTGCAC GGGGGTGCGGGCGGGCCCGAGCCCCTGTCCCATTTCGAAGTGGAGGTGTGCCAGCTGCCCCGGCCCGGCC TCAGGGGCGTCCTCTTCCGCCGTGTGGCGGGCACCGCCCTGGCCTTCCGCACCCTCGTCACCCGCATCTC CAACGACCTCGAACTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR215461 representing NM_172279
Red=Cloning site Green=Tags(s) MSSRTALAPGNDRNSDTHGTLGSGRSSDKGPSWSSRSLGARCRNSIASCPEEQPHVGNYRLLRTIGKGNF AKVKLARHILTGREVAIKIIDKTQLNPSSLQKLFREVRIMKGLNHPNIVKLFEVIETEKTLYLVMEYASA GEVFDYLVSHGRMKEKEARAKFRQIVSAVHYCHQKNIVHRDLKAENLLLDAEANIKIADFGFSNEFTLGS KLDTFCGSPPYAAPELFQGKKYDGPEVDIWSLGVILYTLVSGSLPFDGHNLKELRERVLRGKYRVPFYMS TDCESILRRFLVLNPAKRCTLEQIMKDKWINIGYEGEELKPYTEPEEDFGDTKRIEVMVGMGYTREEIKE ALTNQKYNEVTATYLLLGRKTEEGGDRGAPGLALARVRAPSDTTNGTSSSKGSSHNKGQRASSSTYHRQR RHSDFCGPSPAPLHPKRSPTSTGDTELKEERMPGRKASCSAVGSGSRGLPPSSPMVSSAHNPNKAEIPER RKDSTSTPNNLPPSMMTRRNTYVCTERPGSERPSLLPNGKENSSGTSRVPPASPSSHSLAPPSGERSRLA RGSTIRSTFHGGQVRDRRAGSGSGGGVQNGPPASPTLAHEAAPLPSGRPRPTTNLFTKLTSKLTRRVTDE PERIGGPEVTSCHLPWDKTETAPRLLRFPWSVKLTSSRPPEALMAALRQATAAARCRCRQPQPFLLACLH GGAGGPEPLSHFEVEVCQLPRPGLRGVLFRRVAGTALAFRTLVTRISNDLEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172279 |
| ORF Size | 2256 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172279.2 |
| RefSeq Size | 2259 bp |
| RefSeq ORF | 2259 bp |
| Locus ID | 232944 |
| UniProt ID | Q8CIP4 |
| MW | 83.1 kDa |
| Gene Summary | Serine/threonine-protein kinase (By similarity). Phosphorylates the microtubule-associated protein MAPT/TAU (By similarity). Also phosphorylates the microtubule-associated proteins MAP2 and MAP4 (By similarity). Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles (By similarity). Required for the initiation of axoneme extension during cilium assembly (By similarity). Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro (By similarity). Plays a role in cell cycle progression, specifically in the G1/S checkpoint (By similarity). Reduces neuronal cell survival (By similarity). Plays a role in energy homeostasis by regulating satiety and metabolic rate (PubMed:22992738). Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway (PubMed:24989893). Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221332 | Mark4 (untagged) - Mouse MAP/microtubule affinity-regulating kinase 4 (Mark4), (10ug) |
CNY 9,690.00 |
|
| MG215461 | Mark4 (tGFP-tagged) - Mouse MAP/microtubule affinity-regulating kinase 4 (Mark4), (10ug) |
CNY 7,220.00 |
|
| MR215461L3 | Lenti ORF clone of Mark4 (Myc-DDK-tagged) - Mouse MAP/microtubule affinity-regulating kinase 4 (Mark4) |
CNY 8,460.00 |
|
| MR215461L4 | Lenti ORF clone of Mark4 (mGFP-tagged) - Mouse MAP/microtubule affinity-regulating kinase 4 (Mark4) |
CNY 8,460.00 |
