Setd1a (NM_178029) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BC010250; KMT2F; mKIAA0339; mNSC1; Nsccn1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR215352 representing NM_178029, codon optimized. Due to the complexity of NM_178029, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCAGGAAGGTGGGGGAGACGGGCAGAAAGCCCCGAGCTTCCAGTGGCGGAACTACAAGCTCATAG TGGATCCTGCTTTGGACCCTGCTTTGCGCAGGCCTTCCCAGAAGGTGTACCGCTATGACGGAGTCCACTT CAGTGTCAGTGACTCAAAGTATACCCCCGTGGAAGACCTCCAGGACCCCCGCTGTCATGTCAGGTCCAAA GCCAGAGACTTTTCCCTCCCGGTTCCTAAGTTTAAGCTGGATGAGTTCTATATTGGACAGATACCACTGA AGGAAGTAACTTTTGCGAGGCTCAATGACAATGTGAGGGAAACCTTCCTGAAGGATATGTGCCGGAAATA TGGAGAGGTGGAAGAAGTGGAGATCCTTCTTCATCCCCGCACTCGAAAGCACCTAGGCCTGGCCCGTGTG CTCTTCACCAGCACTCGGGGCGCCAAGGAAACTGTCAAAAACCTCCACCTCACCTCTGTCATGGGCAACA TCATTCATGCCCAGCTTGATATCAAAGGCCAACAACGAATGAAGTACTATGAATTGATTGTCAATGGCTC TTACACTCCTCAGACTGTGCCCACTGGGGGCAAGGCCTTGAGTGAGAAGTTCCAGGGTTCTGGTGCAGCC GCTGAGACGACTGAAGCCCGCCGACGGTCTTCCTCGGACACAGCTGCCTATCCAGCAGGCACTACTGTGG GGGGCACCCCTGGCAATGGCACTCCCTGCTCCCAAGATACAAATTTCTCCAGCAGCCGACAAGATACTCC ATCTTCCTTTGGCCAGTTCACCCCTCAGTCCTCCCAAGGAACCCCCTACACATCTCGGGGTAGCACCCCC TACTCTCAGGACTCTGCCTACTCCAGCAGCACCACTTCAACCTCCTTCAAACCCCGGCGGTCAGAGAACA GCTATCAAGATTCTTTTTCTCGCCGCCATTTCTCTACATCTTCAGCCCCTGCAACCACCGCTACTGCCAC CTCAGCAACTGCTGCAGCTACTGCAGCCTCCTCCTCATCTTCCTCCTCCTCGTCATCCAGCAGCAGCAGC AGCTCTAGCTCTTCAGCCTCCCAGTTTAGAGGCTCCGACTCATCCTATCCTGCCTATTATGAATCATGGA ACAGATATCAAAGGCATACTTCCTACCCTCCGCGCCGCGCCACCCGCGAGGATCCGTCCGGAGCTAGCTT TGCTGAGAACACAGCAGAGCGCTTTCCTCCCAGTTACACGTCCTATCTTGCCCCTGAGCCAAATCGGTCA ACTGACCAAGACTACCGACCTCCCGCCAGTGAAGCTCCCCCACCAGAGCCACCTGAGCCTGGAGGAGGTG GAGGGGGGAGTGGCGGGGGGGGCGGCGGAGGGGGGGGCGGTGGAGGCGGGGCTCCATCTCCAGAACGGGA GGAAGCACGCACGCCTCCCCGCCCAGCCAGCCCCGCAAGGAGCGGTTCTCCCGCTCCTGAGACAACAAAC GAGAGCGTACCCTTTGCACAACACTCTTCTCTCGACTCCCGCATTGAGATGTTGTTGAAGGAGCAGAGGA GCAAATTCAGTTTTTTGGCATCTGACACCGAAGAAGAGGAGGAGAATTCTTCAGCCGGGCCGGGCGCCCG GGATGCTGGTGCCGAGGTGCCAAGCGGAGCCGGACATGGACCCTGCACACCGCCCCCCGCCCCAGCGAAC TTTGAAGATGTCGCCCCGACAGGGTCTGGGGAACCAGGAGCCGCAAGGGAAAGCCCCAAAGCAAACGGGC AAAATCAGGCCTCTCCCTGTTCATCTGGCGAGGACATGGAAATCTCAGATGACGACAGAGGCGGATCCCC ACCCCCCGCCCCAACACCCCCACAGCAGCCTCCACCTCCACCACCACCTCCTCCACCTCCGCCACCACCC TACCTGGCCTCACTGCCTTTGGGATACCCACCCCACCAGCCAGCTTACCTTCTGCCCCCGCGACCTGATG GTCCCCCCCCCCCGGAATACCCTCCCCCACCCCCGCCACCACCCCCCCATATTTATGACTTCGTAAATTC TCTGGAACTTATGGACCGGCTGGGCGCCCAGTGGGGAGGGATGCCAATGTCCTTTCAGATGCAGACACAG ATGCTGACAAGACTGCATCAGCTTCGCCAAGGGAAGGGGCTCACGGCTGCAAGCGCTGGGCCGCCTGGGG GGGCTTTCGGCGAGGCCTTTTTGCCTTTCCCACCTCCACAAGAAGCAGCCTACGGACTGCCCTATGCTCT GTACACCCAAGGACAGGAGGGGCGAGGCTCTTATAGCCGCGAGGCCTACCATCTTCCTTTGCCTATGGCT GCTGAACCGTTGCCCAGCTCTTCCGTGTCAGGGGAGGAAGCCAGACTCCCGCACCGCGAAGAGGCTGAGA TTGCAGAATCCAAGGTGTTGCCTTCCGCTGGAACAGTGGGGCGGGTGTTGGCCACACTTGTGCAGGAAAT GAAGTCTATCATGCAGCGAGATCTGAACAGGAAAATGGTGGAGAACGTCGCATTTGGCGCCTTCGACCAG TGGTGGGAATCAAAAGAAGAGAAAGCTAAGCCCTTTCAGAACGCAGCCAAGCAGCAGGCGAAGGAGGAGG ATAAAGAGAAGATGAAACTGAAAGAACCTGGCATGCTGTCTCTCGTCGACTGGGCTAAAAGCGGAGGGAT CACCGGAATTGAGGCTTTCGCTTTTGGCAGCGGTCTGCGGGGGGCACTCCGCCTGCCGAGTTTCAAAGTA AAGAGGAAGGAGCCAAGCGAGATCTCCGAGGCGAGCGAAGAGAAACGCCCTAGGCCATCAACACCTGCCG AAGAGGACGAAGACGATCCAGAAAGAGAGAAGGAAGCTGGGGAGCCAGGCCGCCCTGGAACTAAACCGCC AAAGAGAGACGAGGAGCGCGGGAAGACGCAGGGCAAGCACAGGAAGTCTTTTACCTTGGATTCTGAAGGT GAGGAGGCGTCCCAGGAGTCTTCCTCAGAGAAGGATGAGGATGATGATGATGAAGATGAGGAAGATGAAG AACAGGAGGAAGCTGTGGATGCCACCAAGAAGGAAGCAGAGGCATCGGATGGTGAGGATGAGGACAGTGA CTCGTCCTCCCAGTGCTCTCTGTATGCGGATTCAGATGGTGAAAATGGCAGCACATCAGACTCCGAGAGT GGCAGCTCGTCTTCCTCTTCCTCATCGTCTTCCTCCTCTTCCTCCTCTTCCTCATCAGAGTCCTCCTCTG AGGAAGAGGAGCAGTCAGCAGTCATTCCCTCAGCTTCACCCCCCAGAGAAGTTCCAGAGCCCCTTCCAGC TCCTGATGAGAAGCCTGAGACAGACGGGCTTGTAGATTCCCCTGTCATGCCTCTCTCTGAAAAGGAGACA TTGCCCACACAGCCTGCAGGCCCTGCTGAAGAACCACCTCCTAGTGTACCACAGCCTCCAGCAGAGCCAC CCGCCGGACCGCCAGACGCTGCCCCCCGCCTGGATGAGCGCCCCTCTTCTCCTATCCCTCTCCTACCCCC ACCCAAGAAACGCCGGAAAACTGTTTCCTTCTCTGCTGCAGAGGAGGCACCAGTCCCAGAACCTTCCACA GCTGCCCCACTGCAGGCCAAGTCTTCTGGTCCAGTCTCTCGAAAAGTCCCCCGGGTCGTAGAGCGGACCA TTCGTAACCTCCCCCTGGACCATGCGTCTCTGGTCAAGAGTTGGCCTGAGGAGGTCGCCCGAGGGGGTCG GAATCGAGCTGGAGGTCGTGTGCGCTCTACTGAAGAAGAGGAGGCCACTGAGTCAGGGACAGAAGTAGAC CTGGCAGTACTGGCTGACCTGGCCCTGACCCCAGCCCGACGTGGGTTGGCTACCCTACCCACTGGTGACG ACTCAGAGGCCACAGAGACCTCAGATGAGGCTGAGCGCCCAAGCCCTCTACTCAGCCACATCCTCTTGGA ACACAACTATGCCCTAGCCATCAAGCCCCCGCCTACCACACCAGCACCTCGGCCCCTGGAGCCAGCTCCT GCCCTTGCTGCACTCTTCAGCTCTCCAGCTGATGAAGTCTTAGAGGCCCCTGAGGTGGTGGTGGCTGAGG CAGAGGAGCCAAAGCAACAGCTGCAGCAGCAGCATCCAGAGCAGGAAGGAGAAGAGGAGGAGGAAGATGA AGAGGAGGAGTCCGAGTCTTCAGAAAGCAGCAGCAGCAGCAGCAGTGATGAGGAAGGGGCAATCAGGAGG CGTAGCCTCCGCTCCCACACTCGAAGAAGAAGGCCACCACTCCCACCCCCACCCCCACCTCCTCCTTCCT TTGAGCCGAGAAGTGAATTTGAGCAGATGACCATCTTGTATGATATTTGGAACTCAGGCCTTGACTTGGA AGACATGAGCTATTTGCGACTCACATATGAACGGCTGCTGCAGCAAACCAGTGGCGCTGACTGGCTTAAT GACACCCACTGGGTCCAGCACACAATCACCAACCTAAGCACTCCAAAGCGCAAAAGACGGCCCCAAGATG GGCCCCGGGAGCATCAGACAGGCTCTGCACGCAGCGAAGGTTATTACCCCATTAGCAAGAAGGAGAAAGA TAAATACTTGGATGTGTGCCCCGTCTCGGCCCGGCAGCTGGAAGGTGGAGACACTCAGGGGACTAATCGG GTGCTTTCTGAGCGACGGTCAGAGCAGCGGCGGTTACTAAGCGCTATTGGCACCTCAGCCATCATGGACA GTGATCTGCTAAAGCTAAACCAGCTCAAGTTTCGGAAGAAGAAACTCCGATTTGGCCGGAGCCGTATCCA TGAGTGGGGTCTGTTTGCCATGGAACCCATTGCCGCTGATGAGATGGTGATCGAATATGTTGGCCAGAAC ATCCGCCAGATGGTAGCGGACATGCGGGAAAAGCGCTACGTGCAGGAGGGCATTGGCAGCAGCTACCTTT TCCGGGTGGATCACGACACCATCATTGATGCCACCAAGTGTGGCAACCTCGCCAGGTTCATCAACCACTG CTGCACGCCCAACTGCTATGCGAAGGTGATCACCATTGAGTCTCAGAAAAAGATTGTCATCTACTCGAAG CAGCCCATCGGCGTGGATGAGGAGATCACCTACGACTACAAGTTCCCACTAGAAGACAACAAGATTCCGT GTCTGTGCGGCACTGAAAGTTGCCGTGGCTCCCTCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR215352 representing NM_178029 Red=Cloning site Green=Tags(s) MDQEGGGDGQKAPSFQWRNYKLIVDPALDPALRRPSQKVYRYDGVHFSVSDSKYTPVEDLQDPRCHVRSK ARDFSLPVPKFKLDEFYIGQIPLKEVTFARLNDNVRETFLKDMCRKYGEVEEVEILLHPRTRKHLGLARV LFTSTRGAKETVKNLHLTSVMGNIIHAQLDIKGQQRMKYYELIVNGSYTPQTVPTGGKALSEKFQGSGAA AETTEARRRSSSDTAAYPAGTTVGGTPGNGTPCSQDTNFSSSRQDTPSSFGQFTPQSSQGTPYTSRGSTP YSQDSAYSSSTTSTSFKPRRSENSYQDSFSRRHFSTSSAPATTATATSATAAATAASSSSSSSSSSSSSS SSSSSASQFRGSDSSYPAYYESWNRYQRHTSYPPRRATREDPSGASFAENTAERFPPSYTSYLAPEPNRS TDQDYRPPASEAPPPEPPEPGGGGGGSGGGGGGGGGGGGGAPSPEREEARTPPRPASPARSGSPAPETTN ESVPFAQHSSLDSRIEMLLKEQRSKFSFLASDTEEEEENSSAGPGARDAGAEVPSGAGHGPCTPPPAPAN FEDVAPTGSGEPGAARESPKANGQNQASPCSSGEDMEISDDDRGGSPPPAPTPPQQPPPPPPPPPPPPPP YLASLPLGYPPHQPAYLLPPRPDGPPPPEYPPPPPPPPPHIYDFVNSLELMDRLGAQWGGMPMSFQMQTQ MLTRLHQLRQGKGLTAASAGPPGGAFGEAFLPFPPPQEAAYGLPYALYTQGQEGRGSYSREAYHLPLPMA AEPLPSSSVSGEEARLPHREEAEIAESKVLPSAGTVGRVLATLVQEMKSIMQRDLNRKMVENVAFGAFDQ WWESKEEKAKPFQNAAKQQAKEEDKEKMKLKEPGMLSLVDWAKSGGITGIEAFAFGSGLRGALRLPSFKV KRKEPSEISEASEEKRPRPSTPAEEDEDDPEREKEAGEPGRPGTKPPKRDEERGKTQGKHRKSFTLDSEG EEASQESSSEKDEDDDDEDEEDEEQEEAVDATKKEAEASDGEDEDSDSSSQCSLYADSDGENGSTSDSES GSSSSSSSSSSSSSSSSSSESSSEEEEQSAVIPSASPPREVPEPLPAPDEKPETDGLVDSPVMPLSEKET LPTQPAGPAEEPPPSVPQPPAEPPAGPPDAAPRLDERPSSPIPLLPPPKKRRKTVSFSAAEEAPVPEPST AAPLQAKSSGPVSRKVPRVVERTIRNLPLDHASLVKSWPEEVARGGRNRAGGRVRSTEEEEATESGTEVD LAVLADLALTPARRGLATLPTGDDSEATETSDEAERPSPLLSHILLEHNYALAIKPPPTTPAPRPLEPAP ALAALFSSPADEVLEAPEVVVAEAEEPKQQLQQQHPEQEGEEEEEDEEEESESSESSSSSSSDEEGAIRR RSLRSHTRRRRPPLPPPPPPPPSFEPRSEFEQMTILYDIWNSGLDLEDMSYLRLTYERLLQQTSGADWLN DTHWVQHTITNLSTPKRKRRPQDGPREHQTGSARSEGYYPISKKEKDKYLDVCPVSARQLEGGDTQGTNR VLSERRSEQRRLLSAIGTSAIMDSDLLKLNQLKFRKKKLRFGRSRIHEWGLFAMEPIAADEMVIEYVGQN IRQMVADMREKRYVQEGIGSSYLFRVDHDTIIDATKCGNLARFINHCCTPNCYAKVITIESQKKIVIYSK QPIGVDEEITYDYKFPLEDNKIPCLCGTESCRGSLN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_178029
ORF Size	5148 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_178029.3, NP_821172.2
RefSeq Size	5934 bp
RefSeq ORF	5151 bp
Locus ID	233904
UniProt ID	E9PYH6
MW	186.1 kDa
Gene Summary	Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overlapping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.[UniProtKB/Swiss-Prot Function]

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