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Phlpp (BC059254) Mouse Tagged ORF Clone
CAT＃: MR211767
- TrueORF®
Phlpp (Myc-DDK-tagged) - Mouse PH domain and leucine rich repeat protein phosphatase (cDNA clone MGC:67583 IMAGE:6406203)
ORF Plasmid: tGFP
"BC059254" in other vectors (4)
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CNY 11,780.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SCOP, mKIAA0606 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211767 representing BC059254
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTTGCCAGTGAACCGATGGACAAGACGACAGGTCATTCTGTGCGGGACGTGCTTGATAGTGTCGT CCGTGAAAGACAGCGTGAGTGGGAAGATGCATGTCCTGCCCCTCATTGGTGGAAAAGTGGAGGAAGTGAA AAAGCACCAGCACTGTTTAGCGTTCAGCTCTTCGGGGCCTCAAAGCCAGACTTACTACATCTGCTTTGAT ACTTTCACGGAGTACTTACGGTGGCTGCGGCAAGTCTCCAAGGTTGCATCACAGCGTATAAGCTCAGTAG ACCTCTCCTGTTGTAGCCTTGAACATCTGCCTGCCAACCTCTTTTACAGCCAAGACCTTACTCATCTCAA CTTAAAACAGAACTTCCTAAGGCAAACCCCCACCCTCCCAGCTGCCAGAGGACTTGGTGAACTGCAAAGG TTCACCAAATTGAAGAGCCTTAACCTTTCCAATAACCACCTAGGAGCCTTTCCGTCAGCAGTCTGCAGCA TTCCAACCCTAGCAGAGCTGAATGTATCTTGCAATGCCCTGCGAGAAGTCCCAGCAGCTGTTGGAGATAT GCAGAACTTGCAGACGTTTCTGCTGGATGGAAATTTTCTCCAGTCCCTTCCTGCTGAACTGGAGAGCATG CACCAGCTCAGCTATCTGGGTCTTTTCTTTAATGAATTCACTGACATTCCAGAGGTATTGGAGAAGCTGA CTGCTGTGGATAAGCTGTGTATGGCTGGGAACTGTGTGGAGACCCTCAGACTACAGGCCTTAAGAAGAAT GCCTCATATTAAACATGTGGACCTAAGACTGAACATACTCAGAAAGCTTATGGCAGATGAGGTGGACTTT GTGCAACATGTCACTCAGCTTGACCTGCGAGACAATAAGCTTGGTGATCTAGATGCTATGATCTTCAACA ACATAGAAGTTCTGCACTGTGAGAGGAATCAGCTGGTGACATTGAACGTTTGTGGCTATTTCCTAAAGGC ACTCTATGCTTCTTCTAATGAACTTGCTCAACTTGATGTCTACCCAGTTCCGAATTATCTCTCTTACATG GATGTCTCAAGAAACTGCCTAGAAAGTGTGCCTGAGTGGGTATGTGAAAGCCGGAAATTAGAAGTTTTGG ATATTGGCCATAATCAAATATGTGAACTTCCTGCCCGCCTGTTTTGTAATAGTAGTCTCCGGAAATTGCT AGCAGGACACAACCGGTTGGCAAGGCTTCCTGAAAGGCTGGAGAGAACTTCTGTGGAGGTCTTGGATGTC CAGCACAACCAGATCACTGAGCTCCCACCAAACCTCCTCATGAAGGCTGACAGCCTGAGATTCCTGAATG CATCTGCAAACAAACTGGAAACCCTGCCTCCAGCCACACTTTCTGAGGAGACGAGCAGTATATTACAGGA GTTGTACCTGACAAACAACTGCCTCACGGATAAGTGTGTGCCCTTGTTAACAGGGCACCCCCGTCTGAAG ATCCTACACATGGCCTATAACCGGCTTCAGAGCTTCCCGGCAAGTAAAATGGCAAAACTGGAGGAACTTG AAGAAATTGATATCAGTGGGAATAAGCTGAAAGCCATCCCCACAACAATCATGAACTGCAGACGCATGCA CACCGTGATTGCTCACTCCAATTGTATCGAAGTCTTTCCTGAAGTTATGCAGCTCCCAGAAGTCAAGTGT GTAGATCTGAGCTGTAATGAGCTGAGTGAAATCACGTTACCAGAAAACCTGCCACCTAAGCTGCAGGAGC TAGACCTGACTGGAAATCCACGCCTTGCCCTGGACCACAAAAGCCTGGAGCTGCTCAATAACATTCGCTG CTTCAAGATTGACCAGCCGTCAGCAGGAGATGCATCAGGAGCCCCAGCAGTATGGAGTCATGGTTACACT GAAGCATCGGGAGTAAAAAACAAGCTGTGTGTCGCAGCCCTGTCTGTAAATAACTTCCGTGACAACCGAG AGGCCCTCTATGGTGTGTTTGATGGAGACCGGAATGTGGAGGTGCCCTACCTTCTCCAGTGCACCATGAG TGACATTTTGGCTGAAGAGCTTCAGAAAACGAAAAATGAAGAAGAATACATGGTCAATACATTCATAGTC ATGCAAAGGAAACTGGGAACTGCTGGGCAGAAACTTGGTGGTGCTGCTGTCCTCTGCCACATCAAGCCTG ACCCTGTGGACCTGGGAGGATCCTTTACCCTGACCTCTGCTAATGTTGGCAAGTGCCAAACGGTTCTCTG TCGAAATGGGAAGCCACTGTCTCTGTCCAGGTCATACATCATGAGCTGTGAAGAAGAGCGGAAGAGGATT AAGCAGCACAAAGCCATCATCACTGAGGATGGCAAGGTCAATGGAGTGACAGAGTCCACGCGCATCCTGG GCTACACCTTCCTCCATCCCAGTGTGGTGCCTCGCCCCCATGTGCAGTCGGTGCTTCTGACTCCACAGGA TGAGTTTTTCATCCTGGGCAGCAAAGGGCTGTGGGACAGCCTATCCATTGACGAGGCTGTGGAAGCTGTA CGCAATGTGCCAGATGCTCTGGCTGCTGCTAAGAAGCTCTGCACCCTGGCTCAGAGCTATGGCTGCCATG ACAGCATCAGTGCTGTGGTGGTACAGCTAAGTGTCACCGAAGACAGCTTCTGCTGCTGTGAGCTCAGTGC TGGAGGGAGCATGCCACCACCCAGCCCCGGAATCTTCCCACCATCCGTCAACATGGTCATCAAGGACCGA CCCTCAGATGGGCTGGGTGTGCCATCCTCCAGCAGTGGCATGGCATCTGAGATCAGCAGTGAACTGTCTA CCTCCGAGATGAGTAGTGAGGTGGGCTCCACAGCCTCTGATGAGCCCCCGTCTGGAGTCCTGAATGAGAG CAGCCCTGCCTACCCCAACGAGCAGCGCTGCATGCTCCACCCTGTCTGCCTGTCTAACTCCTTCCAACGT CAGCTGTCCAGCGCTACTTTCTCCAGCGCGTTCTCTGACAACGGCCTTGACAGTGACGATGAAGAACCCA TTGAGGGGGTGTTCAGCAACGGCAGCCGGGTTGAGGTGGAAGTAGACATCCACTGCAGCAGGGCCAAGGA AAAGGAGAGACAGCAGCACCTACTTCAGGTGCCAGCTGAGGCCAGTGATGAGGGCATTGTCATCAGTGCC AATGAGGATGAGTCAGGTCTGTCCAAGAAGGCAGACTTTTCTGCTGTGGGAACCATTGGACGACGCAGGG CTAATGGCTCTGTAGCTCCCCAGGAAAGGAGCCATAATGTGATAGAGGTTGCTGCAGATGCGCCTCTCCG GAAGCCAGGAGGCTATTTTGCAGCCCCTGCTCAGCCAGATCCAGATGATCAGTTTATCATCCCCCCAGAG CTGGAAGAGGAAGTCAAAGAAATCATGAAACATCACCAGGAGCAGCAGCAGCAGCAGCAGCAGCAGCAGC TGCCACCACCACCCCAGCCGCCACAGCCACAGCCACAGCCACAGCCACAGCCACAGCCACAGCCACAGCG GCACTTCCAAATGGATCACCTGCCAGACTGTTACGATACACCGCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211767 representing BC059254
Red=Cloning site Green=Tags(s) MQLPVNRWTRRQVILCGTCLIVSSVKDSVSGKMHVLPLIGGKVEEVKKHQHCLAFSSSGPQSQTYYICFD TFTEYLRWLRQVSKVASQRISSVDLSCCSLEHLPANLFYSQDLTHLNLKQNFLRQTPTLPAARGLGELQR FTKLKSLNLSNNHLGAFPSAVCSIPTLAELNVSCNALREVPAAVGDMQNLQTFLLDGNFLQSLPAELESM HQLSYLGLFFNEFTDIPEVLEKLTAVDKLCMAGNCVETLRLQALRRMPHIKHVDLRLNILRKLMADEVDF VQHVTQLDLRDNKLGDLDAMIFNNIEVLHCERNQLVTLNVCGYFLKALYASSNELAQLDVYPVPNYLSYM DVSRNCLESVPEWVCESRKLEVLDIGHNQICELPARLFCNSSLRKLLAGHNRLARLPERLERTSVEVLDV QHNQITELPPNLLMKADSLRFLNASANKLETLPPATLSEETSSILQELYLTNNCLTDKCVPLLTGHPRLK ILHMAYNRLQSFPASKMAKLEELEEIDISGNKLKAIPTTIMNCRRMHTVIAHSNCIEVFPEVMQLPEVKC VDLSCNELSEITLPENLPPKLQELDLTGNPRLALDHKSLELLNNIRCFKIDQPSAGDASGAPAVWSHGYT EASGVKNKLCVAALSVNNFRDNREALYGVFDGDRNVEVPYLLQCTMSDILAEELQKTKNEEEYMVNTFIV MQRKLGTAGQKLGGAAVLCHIKPDPVDLGGSFTLTSANVGKCQTVLCRNGKPLSLSRSYIMSCEEERKRI KQHKAIITEDGKVNGVTESTRILGYTFLHPSVVPRPHVQSVLLTPQDEFFILGSKGLWDSLSIDEAVEAV RNVPDALAAAKKLCTLAQSYGCHDSISAVVVQLSVTEDSFCCCELSAGGSMPPPSPGIFPPSVNMVIKDR PSDGLGVPSSSSGMASEISSELSTSEMSSEVGSTASDEPPSGVLNESSPAYPNEQRCMLHPVCLSNSFQR QLSSATFSSAFSDNGLDSDDEEPIEGVFSNGSRVEVEVDIHCSRAKEKERQQHLLQVPAEASDEGIVISA NEDESGLSKKADFSAVGTIGRRRANGSVAPQERSHNVIEVAADAPLRKPGGYFAAPAQPDPDDQFIIPPE LEEEVKEIMKHHQEQQQQQQQQQLPPPPQPPQPQPQPQPQPQPQPQRHFQMDHLPDCYDTPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC059254 |
| ORF Size | 3546 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC059254.1 |
| RefSeq Size | 4783 bp |
| RefSeq ORF | 3548 bp |
| Locus ID | 98432 |
| MW | 175.3 kDa |
| Gene Summary | Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT2 and AKT3, 'Ser-660' of PRKCB and 'Ser-657' of PRKCA (By similarity). Isoform 2 seems to have a major role in regulating Akt signaling in hippocampal neurons (By similarity). Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and suppression of tumor growth. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis. Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation. Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylates RAF1 inhibiting its kinase activity. May act as a negative regulator of K-Ras signaling in membrane rafts (By similarity). Involved in the hippocampus-dependent long-term memory formation (PubMed:17382888). Involved in circadian control by regulating the consolidation of circadian periodicity after resetting (PubMed:20080691). Involved in development and function of regulatory T-cells (PubMed:21498666).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223110 | Phlpp (untagged) - Mouse PH domain and leucine rich repeat protein phosphatase (cDNA clone MGC:67583 IMAGE:6406203), (10ug) |
CNY 12,920.00 |
|
| MG211767 | Phlpp (tGFP-tagged) - Mouse PH domain and leucine rich repeat protein phosphatase (cDNA clone MGC:67583 IMAGE:6406203) |
CNY 12,920.00 |
|
| MR211767L3 | Lenti ORF clone of Phlpp (Myc-DDK-tagged) - Mouse PH domain and leucine rich repeat protein phosphatase (cDNA clone MGC:67583 IMAGE:6406203) |
CNY 13,680.00 |
|
| MR211767L4 | Lenti ORF clone of Phlpp (mGFP-tagged) - Mouse PH domain and leucine rich repeat protein phosphatase (cDNA clone MGC:67583 IMAGE:6406203) |
CNY 13,680.00 |
