Heatr5b (BC086652) Mouse Tagged ORF Clone
CAT＃: MR211590
- TrueORF®
Heatr5b (Myc-DDK-tagged) - Mouse HEAT repeat containing 5B (cDNA clone MGC:77977 IMAGE:6856641)
ORF Plasmid: tGFP
"BC086652" in other vectors (4)
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CNY 7480.00
Product images
CNY 300.00
CNY 6840.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2010013B10Rik; A230048G03Rik; AV071430; D330050P16Rik; mKIAA1414 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211590 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTAGCTCACAGTTTATTGCTAAATGAGGAAGCTTTGGCTCAGATCACGGAGGCAAAGAGACCCG TGTTCATCTTTGAATGGTTGAGATTTCTTGATAAAGTCCTAGTTGCTGCCAACAAGACAGATGTAAAAGA AAAACAGAAAAAACTTGTTGAACAATTAACTGGATTAATAAGCAGCTCACCTGGACCACCTACTCGAAAA TTGTTAGCTAAAAACCTTGCAGCCCTTTATAGCATTGGGGATACTTACACAGTTTTTCAAACACTAGATA AATGTAATGACATTATCAGAAGTAAGGACGACACTGCAGCCTACTTACCAACAAAATTGGCAGCAGTGGC TTGTGTTGGAGCCTTCTATGAGAAGATGGGAAGGATGTTGGGCAGTGCATTTCCAGAAACAGTGAACAAT CTGTTGAAATCTCTGAAAAGTGCAGAGTCTCAAGGGCGAAGTGAGATCTTAATGAGTCTGCAGAAAGTGC TGACTGGACTGGGCGGCGCAGCAGCTTCCTCTCACCGCGACATTTACAAGAATGCTCGATCCCTCCTGAC CGACAGGTCGATGGCTGTCCGATGTGCAGTGGCCAAGTGCCTTCTGGAACTACAGAATGAAGCTGTGTTC ATGTGGACTGCCGAACTAGAAAATGTAGCTACCCTCTGCTTTAAGGCTCTGGAAAACTCAAATTATGGGG TACGAGTCGCAGTATCTAAGCTACTAGGAACAGTCATGGCTACAGCATTGATGCCGAAACAGGCAACAGT CATGCGGCAAAATGTGAAGCGAGCAACCTTCGATGAAGTCTTAGAACTCATGGCCACGGGATTTCTGCGC GGAGGGTCGGGTTTCCTAAAGAGTGGCGGAGAAATGTTGAAAGTTGGAGGGTCTGTTAATCGGGAAGTGA GAGTTGGTGTGACACAGGCCTATGTTGTCTTTGTGACAACTCTGGGTGGCCAGTGGCTGGAGCGTAGCTT TGCCACATTCTTGTCCCACGTACTTGATCTGGTTTCCCATCCTCGAGCAACACAAACACACGTGGACGCT GTGTACTCCAGGCGATGTGTGTCCTTCATGCTGAGAGCTACCGTGGGCAGTCTGTTAGGTGAGAAAGCCC AGATAGCAGCTGCCAAGGAGATCTGCCAGGCAATCGGAAAGCAGATGAAAGCTGTGGAAGCAGTAGTGAA TGATACGAGCAGTGAAAACAAATCGGGGACAGCAGACATTGCAGCCAGCCAGCACGTCATGGTCTGTGCC CTGCAGGAGCTTGGGAGCCTGGTGCAGAGCCTGAATGCCACCGCCTCTCCTCTTATTCAGGAAGCGTCCA TAGGACTTTTGGAGATTGTGACGTCAGTGCTTCTTCACCCAAGCATGGCTGCTCGGCTTGCTGCTGCCTG GTGTCTGCGTTGTGTAGCTGTGGCGCTGCCCTTCCAGTTGACACCATTTCTGGATAGGTGTGCAGAACGG CTCAACAACCTCAAGACATCCCCAGAAGCTGTTAGTGGATACAGTTTTGCAATGGCCGCCTTGTTGGGTG GAGTGCATCAGTGTCCTTTGGGCATTCCTCATGCCAAAGGAAAGATGGTGGTCAGCATTGCTGAAGATCT GCTACGAACTGCTGCCCAAAACAGCAGGCTGTCCCTGCAGCGCACCCAGGCTGGCTGGCTTCTTCTGGGA GCCCTCATGACTTTAGGACCGTCTGTTGTTCGTTACCATCTACCCAAGATGTTGCTGCTGTGGCGGAACG TGTTCCCTCGCTCTTTAAAGGAACTGGAGGCTGAGAAGGCCCGGGGGGATTCATTCACCTGGCAGGTCAC TCTGGAAGGTCGCGCTGGGGCTCTGTGTGCCATGAGGAGTTTTGTTGCACATTGTCCTGAGCTGCTAACA GAGGATGCAATTAGGAAATTAATGACTCCTATCGAATGTGCCATGACCATGATGTCGCACATTCCATCCG TCATCAAGGCCCATGGAGCCCATCTGAAAGCTAGTGCCGCAATGGTCCGCTTAAGACTTTATGATATCTT GGCTTTATTACCTCCAAAAACTTATGAAGGGTCTTTCAATGCACTTCTTAGAGAGCTGGTGGCAGAGTTC ACTCTGACTGACAACTCGGCCAACACAACTACCTCCCTCCTTCGCTCCCTCTGCCACTATGACGACAGCG TTCTCCTGGGCTCCTGGCTTCAAGAGACTGATCATAAATCCATTGAAGACCAGCTCCAGCCAAACAGTGC ATCTGGCAGTGGGGCTCTGGAACACGACCCTTCTTCCATTTACCTACGGATACCTGCAGGTGAAGCCGTG CCGGGTCCGCTCCCTCTCGGAGTCTCAGTGATTGACGCATCTGTGGCTCTGTTCGGGGTTGTGTTTCCAC ATGTTTCCTATAAACACAGGTTACAAATGCTGGATCACTTTGCTGAATGTGTTAAGCAAGCCAAAGGTGT CCGCCAGCAGGCTGTGCAGCTTAATATATTTACTGCTGTTCTTAGTGCACTGAAGGGCTTAGCTGAAAAC AAGAGTACTTTGGGACCTGAGGAAGTCCGCAAGTCTGCTCTGACACTGGTTATGGGAGCCCTCGACAACC CAAACCCCATCTTACGGTGTGCGGCAGGGGAAGCCCTTGGAAGAATGGCTCAGGTGGTGGGAGAAGCCTC TTTCATTGCCCGAATGGCCCAATACAGCTTTGACAAGTTGAAGTCAGCTCGAGATGTCGTGTCCAGGACT GGTCACTCACTGGCCCTCGGTTGTTTACATCGTTATGTTGGTGGCATAGGATCAGGACAACACCTAAAGA CCAGTGTCAGCATTTTACTGGCTCTAGCTCAAGATGGGACATCCCCTGAAGTTCAGACGTGGTCTCTTCA TTCACTCGCCCTGATAGTGGATTCCAGCGGCCCAATGTACCGTGGGTACGTGGAGCCCACGTTGTCCCTG GTACTCACCTTGCTGCTGACAGTTCCACCTTCACACACAGAAGTTCACCAGTGCCTGGGCAGATGCTTGG GGGCGATCATAACTACTGTTGGACCAGAGCTGCAGGGAAATGCAGCGACCATATCCACCATCCGCTCCTC CTGCTTGGTGGGCTGTGCCATTACCCAGGACCACTCTGACTCCCTTGTTCAGGCAGCTGCCATCTCTTGC CTTCAGCAGCTTCACATGTTTGCCCCCACCACACATTCCACACCGACAATTAAGCTGAAAACAAGCTTTT TT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211590 protein sequence
Red=Cloning site Green=Tags(s) MELAHSLLLNEEALAQITEAKRPVFIFEWLRFLDKVLVAANKTDVKEKQKKLVEQLTGLISSSPGPPTRK LLAKNLAALYSIGDTYTVFQTLDKCNDIIRSKDDTAAYLPTKLAAVACVGAFYEKMGRMLGSAFPETVNN LLKSLKSAESQGRSEILMSLQKVLTGLGGAAASSHRDIYKNARSLLTDRSMAVRCAVAKCLLELQNEAVF MWTAELENVATLCFKALENSNYGVRVAVSKLLGTVMATALMPKQATVMRQNVKRATFDEVLELMATGFLR GGSGFLKSGGEMLKVGGSVNREVRVGVTQAYVVFVTTLGGQWLERSFATFLSHVLDLVSHPRATQTHVDA VYSRRCVSFMLRATVGSLLGEKAQIAAAKEICQAIGKQMKAVEAVVNDTSSENKSGTADIAASQHVMVCA LQELGSLVQSLNATASPLIQEASIGLLEIVTSVLLHPSMAARLAAAWCLRCVAVALPFQLTPFLDRCAER LNNLKTSPEAVSGYSFAMAALLGGVHQCPLGIPHAKGKMVVSIAEDLLRTAAQNSRLSLQRTQAGWLLLG ALMTLGPSVVRYHLPKMLLLWRNVFPRSLKELEAEKARGDSFTWQVTLEGRAGALCAMRSFVAHCPELLT EDAIRKLMTPIECAMTMMSHIPSVIKAHGAHLKASAAMVRLRLYDILALLPPKTYEGSFNALLRELVAEF TLTDNSANTTTSLLRSLCHYDDSVLLGSWLQETDHKSIEDQLQPNSASGSGALEHDPSSIYLRIPAGEAV PGPLPLGVSVIDASVALFGVVFPHVSYKHRLQMLDHFAECVKQAKGVRQQAVQLNIFTAVLSALKGLAEN KSTLGPEEVRKSALTLVMGALDNPNPILRCAAGEALGRMAQVVGEASFIARMAQYSFDKLKSARDVVSRT GHSLALGCLHRYVGGIGSGQHLKTSVSILLALAQDGTSPEVQTWSLHSLALIVDSSGPMYRGYVEPTLSL VLTLLLTVPPSHTEVHQCLGRCLGAIITTVGPELQGNAATISTIRSSCLVGCAITQDHSDSLVQAAAISC LQQLHMFAPTTHSTPTIKLKTSFF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | BC086652 |
| ORF Size | 3222 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC086652, AAH86652 |
| RefSeq Size | 3463 bp |
| RefSeq ORF | 3224 bp |
| Locus ID | 320473 |
| MW | 115.8 kDa |
| Gene Summary | Component of clathrin-coated vesicles (By similarity). Component of the aftiphilin/p200/gamma-synergin complex, which plays roles in AP1G1/AP-1-mediated protein trafficking including the trafficking of transferrin from early to recycling endosomes, and the membrane trafficking of furin and the lysosomal enzyme cathepsin D between the trans-Golgi network (TGN) and endosomes (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223140 | Heatr5b (untagged) - Mouse HEAT repeat containing 5B (cDNA clone MGC:77977 IMAGE:6856641), (10ug) |
CNY 12920.00 |
|
| MG211590 | Heatr5b (tGFP-tagged) - Mouse HEAT repeat containing 5B (cDNA clone MGC:77977 IMAGE:6856641) |
CNY 6650.00 |
|
| MR211590L3 | Lenti ORF clone of Heatr5b (Myc-DDK-tagged) - Mouse HEAT repeat containing 5B (cDNA clone MGC:77977 IMAGE:6856641) |
CNY 7980.00 |
|
| MR211590L4 | Lenti ORF clone of Heatr5b (mGFP-tagged) - Mouse HEAT repeat containing 5B (cDNA clone MGC:77977 IMAGE:6856641) |
CNY 7980.00 |
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