Usp43 (BC021474) Mouse Tagged ORF Clone
CAT＃: MR210839
- TrueORF®
Usp43 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 43 (cDNA clone MGC:38313 IMAGE:5343429)
ORF Plasmid: tGFP
"BC021474" in other vectors (4)
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CNY 6048.00
Product images
CNY 6840.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 9130017K11Rik, B930051C24, RP23-464J2.1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210839 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTGCAGAGGAAGGAGGTGTCCCTGCAGAGGAGGTGATCTTGGTTGAACTGTATCCCAATGGATTCC AGCGGTCATTCTTTGATGAAGAGGACCTGAATACCATTGCAGAGGGTGACAATGTGTATGCTTTCCAAGT CCCTCCCTCACCTGGTCTGGGGACGCTCTCAGCCCATCCGTCTGGTCTGTCAGTGTCACCGCGCTTGCCA GTCCGTGACAGCCAGCGATTCTCTGGGCCTCTCCACAGCGAGAACAGGGTGGTCTTTCTCTTCTGTAACC TGGTGGGCTCAGGGCAGCAGGCGAGCAGGTTTGGACCACCGTTCTTGATTCGGGAAGATAGAACAATCTC CTGGGCCCAGCTTCAACAGTGTATTCTCAGCAAGGTTCGATGCCTCATGAGGAGTGAGGTCTCTGCACAG GACCTGGGGACTCTGTTCTCCATCCGGGTTGTGGGGCTCTCCTTGGCCTGCAGCTACTTATCTCCAAAGG ACAACCGGCCCCTTTGCCACTGGGCAGTTGACAGGGCTTTGCACCTCAGGAGGCCAGGAGGCCCTCCTCA TGTCAAGCTGGCTGTGGAATGGGACAGCTCTGTCACAGAGCGCCTGTTTGGGAGCCTCCAGGAGGAACGG GTCCAGGATGCAGACAGTGTGTGGCGGCAGCAGCAGGCACACCAGCAGCCCAGCTGTACCCTGGACGAAT GTTTTCAGTCCTATACCAAGGAGGAACAGCTGGCCCAGGATGATGCATGGAAGTGCCCTCACTGCCAAGT CCTCCAGCAGGGTGTGGTCAAGCTGAGTCTGTGGACTCTGCCCGACATCCTGATCATCCATCTGAAACGG TTCTGTCAGGTGGGAGAGAGAAGGAACAAGCTCTCCACGCTGGTGAAGTTCCCACTGTCTGGACTCAACA TGGCTCCCCATGTAGCACGGAGGAGCACCAACTCCAAGGCGGGGCCCGGCCCTTGGTCCTCCTGGAAGCA GCCGATCTGCCTCCCCACCACCTACCCGCTGGACTTCCTCTACGACTTGTATGCTGTCTGCAACCACCAT GGCAGTCTGCAAGGTGGGCATTACACAGCCTATTGCCGGAACTCCCTGGACGGCCAGTGGTACAGCTATG ACGACAGCACAGTGGAGGCCCTGCGAGAGGATGAGGTCAACACCAGGGGGGCTTACATCCTATTCTACCA GAAGCGGAACAGCATCCCTCCCTGGTCAGCCAGCAGCTCCATGAGAGGTTCCACCAGCTCCTCCTTGTCT GATCATTGGCTCATGAGACTCGGGAGCCTCAACAACAGCACAAGGGGAAGCTTGCTGTCCTGGAGCTCGG CCCCCTGCCCCTCCGTGGCCCGGGTACCCGACTCTCCTGTCTTCACCAATGGTGTCTGCCACCAGGACAA GGGAGGAGTTGAGACCAGACCTTTGGTTCGGGGTGTTGGTGGCCGAAGTATCAGCATGAAGGCATCTCCT GCTTCCCGATCCAGGCATGGGCCCTTCAAGACCATGCCCCTGAGGTGGTCATTTGGACACAGGGAGAAAC GTCCCGGGGCATCCGTTGAACTGGTGGAATACCTAGAGTCCAGAAGAAGACCCCGTTCTACCAGCCAGTC CATTGTGCCGCTCTTGACTCGTGCTGCTGGTGGGGAAGAGACTTCAGCGTCACCGAGGTCAGATGGCACC CTTCCTGCTAAAAGTGAAGACAGCGGTCGAGCCATTGGGCAAGGAACAACTGGGGTGCCCCTTTCCTCAT GCCACCTCAACCACCATCCAGCCCTGGGGTCTTTAGATGACAGTCTGCACACTGCCAGGACACGAACGGG GAATGTAAGTCAGGATATCAGGCTCCCAAAAAAGTTTGACCTGCCCCTCACTGTGATGCCCTCAGTGGGG GATGAAAAGCCAGCTCGCCCTGAGGGCCAGAAGATGACACCTTGGAAGGGAAGTAGCCAGGTGGGAAGCC AGAGTAGCCCACCTTCCCCCTCCACTGGCTTGCTTAGAAACTTTAAGGACAATGGCCCAGGTACCTTACC CAAGATGAAGTCTAAGGCTGCCATGGAGGAAAGGGCCCCTGGCAAAGACAGGGGACAGGGGACATTCACT CTTTTGAAGTCTGTGTTTTGGAAGAAAGAACACAAGAGGACTGTCAGGACTGAGAGCTCACCACCAGCAC CCCCAATCTCCCTAGGGAGTGACCGGCTGAGCCCTGCCATGAATGAGCAGGCCCGAGGTTCATCCCCTGC CCTCAGGATTCGAGAGAGCCCAGCCAAGGGCCTGGGCAACCACATGGAGAGGGACATCAGATCTGCGCCC AGCTCTCTCCACCTCCCCCGAAAAGCCAGCAGGCCCCCTCGAGCCAGTACAGCTGGCACCTCACAAAGGA CCATTCCTGGGGAACAGATTTCTTATGGCACCCTGCAGAGAGTGAAATACCACACCCTTTCCTTAGGTCG AAAGAAATCCTTACCTGAGTCCAGCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210839 protein sequence
Red=Cloning site Green=Tags(s) MVAEEGGVPAEEVILVELYPNGFQRSFFDEEDLNTIAEGDNVYAFQVPPSPGLGTLSAHPSGLSVSPRLP VRDSQRFSGPLHSENRVVFLFCNLVGSGQQASRFGPPFLIREDRTISWAQLQQCILSKVRCLMRSEVSAQ DLGTLFSIRVVGLSLACSYLSPKDNRPLCHWAVDRALHLRRPGGPPHVKLAVEWDSSVTERLFGSLQEER VQDADSVWRQQQAHQQPSCTLDECFQSYTKEEQLAQDDAWKCPHCQVLQQGVVKLSLWTLPDILIIHLKR FCQVGERRNKLSTLVKFPLSGLNMAPHVARRSTNSKAGPGPWSSWKQPICLPTTYPLDFLYDLYAVCNHH GSLQGGHYTAYCRNSLDGQWYSYDDSTVEALREDEVNTRGAYILFYQKRNSIPPWSASSSMRGSTSSSLS DHWLMRLGSLNNSTRGSLLSWSSAPCPSVARVPDSPVFTNGVCHQDKGGVETRPLVRGVGGRSISMKASP ASRSRHGPFKTMPLRWSFGHREKRPGASVELVEYLESRRRPRSTSQSIVPLLTRAAGGEETSASPRSDGT LPAKSEDSGRAIGQGTTGVPLSSCHLNHHPALGSLDDSLHTARTRTGNVSQDIRLPKKFDLPLTVMPSVG DEKPARPEGQKMTPWKGSSQVGSQSSPPSPSTGLLRNFKDNGPGTLPKMKSKAAMEERAPGKDRGQGTFT LLKSVFWKKEHKRTVRTESSPPAPPISLGSDRLSPAMNEQARGSSPALRIRESPAKGLGNHMERDIRSAP SSLHLPRKASRPPRASTAGTSQRTIPGEQISYGTLQRVKYHTLSLGRKKSLPESSF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC021474 |
| ORF Size | 2478 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC021474, AAH21474 |
| RefSeq Size | 2948 bp |
| RefSeq ORF | 2480 bp |
| Locus ID | 216835 |
| MW | 90.9 kDa |
| Gene Summary | May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220699 | Usp43 (untagged) - Mouse ubiquitin specific peptidase 43 (cDNA clone MGC:38313 IMAGE:5343429), (10ug) |
CNY 9120.00 |
|
| MG210839 | Usp43 (tGFP-tagged) - Mouse ubiquitin specific peptidase 43 (cDNA clone MGC:38313 IMAGE:5343429) |
CNY 5230.00 |
|
| MR210839L3 | Lenti ORF clone of Usp43 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 43 (cDNA clone MGC:38313 IMAGE:5343429) |
CNY 6650.00 |
|
| MR210839L4 | Lenti ORF clone of Usp43 (mGFP-tagged) - Mouse ubiquitin specific peptidase 43 (cDNA clone MGC:38313 IMAGE:5343429) |
CNY 6650.00 |
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
