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Ppargc1a (NM_008904) Mouse Tagged ORF Clone
CAT＃: MR210710
- TrueORF®
Ppargc1a (Myc-DDK-tagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (Ppargc1a), transcript variant 1
ORF Plasmid: tGFP
"NM_008904" in other vectors (6)
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CNY 7,980.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A830037N07Rik; Gm11133; Pgc; PGC-1; Pgc-1alpha; Pgc1; Pgco1; PPARGC-1-alpha; Ppargc1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210710 representing NM_008904
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTGGGACATGTGCAGCCAAGACTCTGTATGGAGTGACATAGAGTGTGCTGCTCTGGTTGGTGAGG ACCAGCCTCTTTGCCCAGATCTTCCTGAACTTGACCTTTCTGAACTTGATGTGAATGACTTGGATACAGA CAGCTTTCTGGGTGGATTGAAGTGGTGTAGCGACCAATCGGAAATCATATCCAACCAGTACAACAATGAG CCTGCGAACATATTTGAGAAGATAGATGAAGAGAATGAGGCAAACTTGCTAGCGGTCCTCACAGAGACAC TGGACAGTCTCCCCGTGGATGAAGACGGATTGCCCTCATTTGATGCACTGACAGATGGAGCCGTGACCAC TGACAACGAGGCCAGTCCTTCCTCCATGCCTGACGGCACCCCTCCCCCTCAGGAGGCAGAAGAGCCGTCT CTACTTAAGAAGCTCTTACTGGCACCAGCCAACACTCAGCTCAGCTACAATGAATGCAGCGGTCTTAGCA CTCAGAACCATGCAGCAAACCACACCCACAGGATCAGAACAAACCCTGCCATTGTTAAGACCGAGAATTC ATGGAGCAATAAAGCGAAGAGCATTTGTCAACAGCAAAAGCCACAAAGACGTCCCTGCTCAGAGCTTCTC AAGTATCTGACCACAAACGATGACCCTCCTCACACCAAACCCACAGAAAACAGGAACAGCAGCAGAGACA AATGTGCTTCCAAAAAGAAGTCCCATACACAACCGCAGTCGCAACATGCTCAAGCCAAACCAACAACTTT ATCTCTTCCTCTGACCCCAGAGTCACCAAATGACCCCAAGGGTTCCCCATTTGAGAACAAGACTATTGAG CGAACCTTAAGTGTGGAACTCTCTGGAACTGCAGGCCTAACTCCTCCCACAACTCCTCCTCATAAAGCCA ACCAAGATAACCCTTTCAAGGCTTCGCCAAAGCTGAAGCCCTCTTGCAAGACCGTGGTGCCACCGCCAAC CAAGAGGGCCCGGTACAGCGAGTGTTCTGGTACCCAAGGCAGCCACTCCACCAAGAAAGGGCCCGAGCAA TCTGAGTTGTACGCACAACTCAGCAAGTCCTCAGGGCTCAGCCGAGGACACGAGGAAAGGAAGACTAAAC GGCCCAGTCTTCGGCTGTTTGGTGACCATGACTATTGTCAGTCACTCAATTCCAAAACGGATATACTCAT TAACATATCACAGGAGCTCCAAGACTCTAGACAACTAGACTTCAAAGATGCCTCCTGTGACTGGCAGGGG CACATCTGTTCTTCCACAGATTCAGGCCAGTGCTACCTGAGAGAGACTTTGGAGGCCAGCAAGCAGGTCT CTCCTTGCAGCACCAGAAAACAGCTCCAAGACCAGGAAATCCGAGCGGAGCTGAACAAGCACTTCGGTCA TCCCTGTCAAGCTGTGTTTGACGACAAATCAGACAAGACCAGTGAACTAAGGGATGGCGACTTCAGTAAT GAACAATTCTCCAAACTACCTGTGTTTATAAATTCAGGACTAGCCATGGATGGCCTATTTGATGACAGTG AAGATGAAAGTGATAAACTGAGCTACCCTTGGGATGGCACGCAGCCCTATTCATTGTTCGATGTGTCGCC TTCTTGCTCTTCCTTTAACTCTCCGTGTCGAGACTCAGTGTCACCACCGAAATCCTTATTTTCTCAAAGA CCCCAAAGGATGCGCTCTCGTTCAAGATCCTTTTCTCGACACAGGTCGTGTTCCCGATCACCATATTCCA GGTCAAGATTAAGGTCCCCAGGCAGTAGATCCTCTTCAAGATCCTGTTACTACTATGAATCAAGCCACTA CAGACACCGCACACACCGCAATTCTCCCTTGTATGTGAGATCACGTTCAAGGTCACCCTACAGCTGTAGG CCCAGGTACGACAGCTATGAAGCCTATGAGCACGAAAGGCTCAAGAGGGATGAATACCGCAAAGAGCACG AGAAGCGGGAGTCTGAAAGGGCCAAGCAGAGAGAGAGGCAGAAGCAGAAAGCAATTGAAGAGCGCCGTGT GATTTACGTTGGTAAAATCAGACCTGACACAACGCGGACAGAATTGAGAGACCGCTTTGAAGTTTTTGGT GAAATTGAGGAATGCACCGTAAATCTGCGGGATGATGGAGACAGCTATGGTTTCATCACCTACCGTTACA CCTGTGACGCTTTCGCTGCTCTTGAGAATGGATATACTTTACGCAGGTCGAACGAAACTGACTTCGAGCT GTACTTTTGTGGACGGAAGCAATTTTTCAAGTCTAACTATGCAGACCTAGATACAAACTCAGACGATTTT GACCCTGCTTCCACCAAGAGCAAGTATGACTCTCTGGATTTTGATAGTTTACTGAAGGAAGCTCAGAGAA GCTTGCGCAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210710 representing NM_008904
Red=Cloning site Green=Tags(s) MAWDMCSQDSVWSDIECAALVGEDQPLCPDLPELDLSELDVNDLDTDSFLGGLKWCSDQSEIISNQYNNE PANIFEKIDEENEANLLAVLTETLDSLPVDEDGLPSFDALTDGAVTTDNEASPSSMPDGTPPPQEAEEPS LLKKLLLAPANTQLSYNECSGLSTQNHAANHTHRIRTNPAIVKTENSWSNKAKSICQQQKPQRRPCSELL KYLTTNDDPPHTKPTENRNSSRDKCASKKKSHTQPQSQHAQAKPTTLSLPLTPESPNDPKGSPFENKTIE RTLSVELSGTAGLTPPTTPPHKANQDNPFKASPKLKPSCKTVVPPPTKRARYSECSGTQGSHSTKKGPEQ SELYAQLSKSSGLSRGHEERKTKRPSLRLFGDHDYCQSLNSKTDILINISQELQDSRQLDFKDASCDWQG HICSSTDSGQCYLRETLEASKQVSPCSTRKQLQDQEIRAELNKHFGHPCQAVFDDKSDKTSELRDGDFSN EQFSKLPVFINSGLAMDGLFDDSEDESDKLSYPWDGTQPYSLFDVSPSCSSFNSPCRDSVSPPKSLFSQR PQRMRSRSRSFSRHRSCSRSPYSRSRLRSPGSRSSSRSCYYYESSHYRHRTHRNSPLYVRSRSRSPYSCR PRYDSYEAYEHERLKRDEYRKEHEKRESERAKQRERQKQKAIEERRVIYVGKIRPDTTRTELRDRFEVFG EIEECTVNLRDDGDSYGFITYRYTCDAFAALENGYTLRRSNETDFELYFCGRKQFFKSNYADLDTNSDDF DPASTKSKYDSLDFDSLLKEAQRSLRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008904 |
| ORF Size | 2391 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008904.1, NM_008904.2, NP_032930.1 |
| RefSeq Size | 3029 bp |
| RefSeq ORF | 2394 bp |
| Locus ID | 19017 |
| UniProt ID | O70343 |
| MW | 91 kDa |
| Gene Summary | This gene encodes a transcriptional coactivator that induces and coordinates gene expression regulating mitochondrial biogenesis, respiration, hepatic gluconeogenesis, thermogenic program in brown fat and muscle fiber-type switching. Mice lacking the encoded protein exhibit reduced thermogenic capacity, hyperactivity and resistance to diet-induced obesity. Mice lacking the encoded protein specifically in the heart exhibit peripartum cardiomyopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
IRX5 regulates adipocyte amyloid precursor protein and mitochondrial respiration in obesity
,Bjune, JI;Haugen, C;Gudbrandsen, O;Nordbø, OP;Nielsen, HJ;Våge, V;Njølstad, PR;Sagen, JV;Dankel, SN;Mellgren, G;,
Int J Obes (Lond) 2018
,PubMed ID 30538277
[PPARGC1A]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204780 | Ppargc1a (untagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (Ppargc1a), transcript variant 1, (10ug) |
CNY 8,768.00 |
|
| MG210710 | Ppargc1a (tGFP-tagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (Ppargc1a) |
CNY 8,740.00 |
|
| MR210710L1 | Lenti ORF clone of Ppargc1a (Myc-DDK-tagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (Ppargc1a), transcript variant 1 |
CNY 9,880.00 |
|
| MR210710L2 | Lenti ORF clone of Ppargc1a (mGFP-tagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (Ppargc1a), transcript variant 1 |
CNY 9,880.00 |
|
| MR210710L3 | Lenti ORF clone of Ppargc1a (Myc-DDK-tagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (Ppargc1a), transcript variant 1 |
CNY 11,152.00 |
|
| MR210710L4 | Lenti ORF clone of Ppargc1a (mGFP-tagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (Ppargc1a), transcript variant 1 |
CNY 9,880.00 |
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