Smc3 (BC057345) Mouse Tagged ORF Clone

CAT#: MR209999

  • TrueORF®

Smc3 (Myc-DDK-tagged) - Mouse structural maintenace of chromosomes 3 (cDNA clone MGC:67063 IMAGE:6811961)

ORF Plasmid: DDK tGFP



  "BC057345" in other vectors (6)

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Mouse Protein / Lysate Request

CNY 7,976.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (3)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms HCAP, SmcD, Bamacan
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR209999 representing BC057345
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATAACTTTGAGTGTGAACCAGCTTTCTATACTTGTGTGGAAGTCACTGCTGGTAACAGGTTATTTT
ATCACATTGTTGATTCAGATGAAGTCAGCACGAAAATTTTAATGGAGTTCAATAAAATGAATCTTCCTGG
AGAGGTGACTTTCCTGCCTCTTAACAAGTTAGATGTGAGAGATACTGCCTATCCTGAAACTAACGATGCT
ATTCCTATGATCAGTAAGCTGAGGTACAATCCCAGATTTGACAAAGCTTTCAAACATGTATTTGGAAAGA
CACTCATCTGTCGGAGCATGGAAGTTTCAACTCAGCTGGCCCGGGCCTTCACTATGGACTGTATTACTTT
GGAAGGTGATCAAGTCAGCCATCGAGGTGCTCTGACTGGAGGTTATTACGACACAAGAAAGTCTCGACTT
GAGTTACAGAAAGACGTTAGGAAGGCAGAGGAGGAGCTGGGTGAGCTGGAGGCTAAGCTCAATGAAAACC
TACGCAGGAACATTGAAAGGATTAATAATGAAATTGACCAGTTGATGAACCAAATGCAGCAGATAGAGAC
CCAACAAAGAAAATTTAAAGCATCCAGAGATAGCATATTATCAGAGATGAAGATGCTAAAAGAGAAGAGA
CAGCAATCAGAAAAGACCTTCATGCCAAAGCAACGTAGCTTACAAAGCTTGGAGGCAAGTCTGCATGCTA
TGGAGTCCACCAGAGAGTCACTGAAAGCGGAGCTAGGAACGGATTTGCTTTCTCAACTCAGTCTGGAAGA
TCAGAAAAGAGTCGACGCTCTGAATGATGAAATCCGTCAGCTGCAGCAGGAAAACAGACAGCTGCTAAAT
GAAAGAATTAAACTAGAAGGCATTATTACTCGAGTAGAGACTTACCTGAATGAGAATCTGAGGAAACGCT
TGGACCAAGTAGAACAGGAACTTAATGAACTGAGAGAGACAGAAGGTGGTACTGTTCTTACTGCCACAAC
ATCAGAACTTGAAGCTATTAATAAAAGAGTAAAAGATACTATGGCAAGATCAGAAGATTTGGACAATTCC
ATTGACAAAACAGAAGCTGGAATTAAAGAGCTCCAGAAAAGTATGGAGCGCTGGAAAAATATGGAGAAAG
AACACATGGATGCCATAAATCATGATACTAAAGAGCTGGAGAAGATGACCAACCGGCAAGGCATGCTGTT
GAAGAAGAAGGAGGAGTGTATGAAGAAGATCCGGGAGCTGGGGTCCCTCCCCCAGGAAGCGTTTGAGAAG
TACCAGACACTGAGCCTGAAGCAGTTGTTTCGAAAACTGGAGCAATGCAACACAGAGTTAAAGAAGTACA
GCCACGTGAACAAAAAGGCTCTAGATCAGTTTGTGAATTTCTCTGAGCAGAAGGAAAAGCTGATAAAGCG
GCAAGAGGAATTGGATAGAGGCTACAAATCAATCATGGAATTGATGAATGTACTTGAACTTAGAAAATAT
GAAGCTATTCAGTTAACTTTCAAACAGGTATCTAAGAACTTCAGTGAAGTTTTCCAGAAGTTGGTACCTG
GTGGCAAAGCGACTTTGGTGATGAAGAAAGGAGATGTGGAGGGCAGCCAGTCTCAGGACGAAGGAGAAGG
GAGTGGCGAGAGTGAGCGGGGCTCTGGGTCACAGAGCAGTGTTCCATCAGTTGACCAGTTCACAGGAGTT
GGGATCAGGGTGTCATTTACAGGGAAGCAAGGGGAGATGAGAGAAATGCAGCAGCTCTCAGGAGGACAGA
AATCTCTGGTAGCCCTTGCTCTCATCTTTGCCATTCAGAAATGCGACCCTGCTCCTTTTTACCTGTTTGA
TGAGATCGACCAAGCTTTGGATGCTCAGCACAGAAAAGCCGTGTCAGATATGATTATGGAGCTAGCCGTG
CATGCCCAGTTTATTACCACTACTTTCAGGCCTGAACTGCTTGAGTCAGCTGACAAATTCTATGGTGTAA
AGTTCAGAAATAAGGTTAGTCACATTGATGTGATCACAGCAGAGATGGCCAAAGATTTTGTAGAAGACGA
TACCACGCATGGT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR209999 representing BC057345
Red=Cloning site Green=Tags(s)

MNNFECEPAFYTCVEVTAGNRLFYHIVDSDEVSTKILMEFNKMNLPGEVTFLPLNKLDVRDTAYPETNDA
IPMISKLRYNPRFDKAFKHVFGKTLICRSMEVSTQLARAFTMDCITLEGDQVSHRGALTGGYYDTRKSRL
ELQKDVRKAEEELGELEAKLNENLRRNIERINNEIDQLMNQMQQIETQQRKFKASRDSILSEMKMLKEKR
QQSEKTFMPKQRSLQSLEASLHAMESTRESLKAELGTDLLSQLSLEDQKRVDALNDEIRQLQQENRQLLN
ERIKLEGIITRVETYLNENLRKRLDQVEQELNELRETEGGTVLTATTSELEAINKRVKDTMARSEDLDNS
IDKTEAGIKELQKSMERWKNMEKEHMDAINHDTKELEKMTNRQGMLLKKKEECMKKIRELGSLPQEAFEK
YQTLSLKQLFRKLEQCNTELKKYSHVNKKALDQFVNFSEQKEKLIKRQEELDRGYKSIMELMNVLELRKY
EAIQLTFKQVSKNFSEVFQKLVPGGKATLVMKKGDVEGSQSQDEGEGSGESERGSGSQSSVPSVDQFTGV
GIRVSFTGKQGEMREMQQLSGGQKSLVALALIFAIQKCDPAPFYLFDEIDQALDAQHRKAVSDMIMELAV
HAQFITTTFRPELLESADKFYGVKFRNKVSHIDVITAEMAKDFVEDDTTHG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC057345
ORF Size 2043 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC057345.1
RefSeq Size 2711 bp
RefSeq ORF 2045 bp
Locus ID 13006
MW 99.3 kDa
Gene Summary Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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