Kars (BC027356) Mouse Tagged ORF Clone
CAT＃: MR209528
- TrueORF®
Kars (Myc-DDK-tagged) - Mouse lysyl-tRNA synthetase (cDNA clone MGC:37041 IMAGE:4950333)
ORF Plasmid: tGFP
"BC027356" in other vectors (4)
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CNY 6270.00
Product images
CNY 6840.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA589550; AL024334; AL033315; AL033367; D8Ertd698e; D8Wsu108e; LysRS; mKIAA0070 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209528 representing BC027356
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGATGCAAGCTGCTGTCAGGCTTGTTAGGGGGGCCCTGCGCCAAACCTCATGGGCAGAATGGGGTC AGAGGGAACTTCGACTGGGCCACCTTGCTCCTTTCACAACGCTCCACAAGGACCAGCCACTTTCTGATAG AAGAAGTGAGCTGAAGAGGCGTCTGAAAGCTGAGAAGAAACTGGCAGAGAAGGAGGCCAAGCAGAAAGAG CTGAGTGAGAAACAGCTAAACCAGACTGCTTCCGCTCCCAACCACACGGCTGACAATGGCGTGGGTGCTG AGGAGGAGACTCTGGACCCAAATCAATACTACAAGATCCGAAGTCAAGCCGTCCAGCAGCTGAAGGTCAC TGGGGAGGATCCGTACCCACACAAGTTCCACGTGGACATCTCACTCACTCAGTTCATCCAAGAATATAGT CACCTGCAGCCTGGGGACCACCTGACTGATGTCACCCTCAAAGTGGCAGGCCGCATCCACGCCAAGAGGG CCTCTGGAGGGAAGCTCATCTTCTATGACCTGCGAGGAGAGGGGGTCAAGTTACAAGTCATGGCCAACTC CAGGAATTACAAATCAGAGGAGGAATTTGTTCATATCAATAACAAACTGCGCCGGGGAGACATAATTGGA GTTGAGGGCAATCCCGGGAAAACCAAGAAGGGCGAGCTGAGCATCATCCCCCAGGAGATCACGCTGCTGT CCCCCTGCTTGCACATGCTGCCTCATCTTCACTTTGGCCTCAAAGACAAGGAAACACGGTATCGTCAAAG ATACTTGGACTTGATCCTGAACGACTTTGTGAGGCAGAAATTTATCGTCCGCTCTAAGATCATCACATAC ATAAGAAGTTTCTTGGATGAGCTGGGCTTCCTAGAGATCGAAACTCCCATGATGAACATCATTCCCGGGG GAGCTGTGGCCAAGCCTTTCATCACCTATCACAATGAGCTGGACATGAACTTGTATATGAGAATTGCTCC AGAACTCTACCACAAGATGCTGGTGGTTGGTGGCATTGACCGGGTTTATGAAATTGGGCGCCAGTTCCGG AACGAAGGGATTGATTTGACTCACAATCCTGAGTTCACCACCTGTGAGTTCTACATGGCCTATGCAGACT ATCATGACCTCATGGAGATCACAGAGAAGATGCTGTCAGGGATGGTGAAGAGCATTACAGGCAGTTACAA GATCACCTACCACCCAGATGGGCCGGAAGGCCAAGCCTACGAGGTCGACTTCACCCCACCCTTCCGAAGA ATCAGCATGGTAGAAGAGCTTGAGAAAGCCCTAGGTGTGAAGCTGCCAGAAACCAGTCTCTTTGAAACTG AAGAAACTCGGAAAATTCTTGATGATATTTGTGTTGCAAAAGCTGTTGAATGCCCCCCACCTCGGACCAC AGCCAGGCTCCTTGATAAGCTCGTTGGCGAGTTCCTCGAAGTCACATGCATCAGCCCTACCTTCATCTGT GATCACCCACAGATCATGAGTCCTTTGGCCAAATGGCACCGCTCCAAAGAGGGTCTCACGGAGCGCTTTG AGCTGTTTGTCATGAAGAAGGAGATATGCAATGCCTATACTGAGCTGAATGACCCCGTGCGGCAGAGGCA GCTGTTTGAGGAGCAGGCCAAGGCCAAGGCTGCCGGTGATGACGAGGCCATGTTCATAGACGAGAACTTC TGTACTGCCCTGGAATACGGGCTGCCTCCCACAGCGGGCTGGGGCATGGGCATTGATCGGCTCACCATGT TTCTCACCGATTCCAACAATATCAAGGAAGTACTTCTGTTTCCTGCCATGAAGCCAGAGGACAAGAAGGA AACTGCAGCGACCACTGAAACCCCAGAGAGCACAGAGGCCAGCCCCTCTGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209528 representing BC027356
Red=Cloning site Green=Tags(s) MLMQAAVRLVRGALRQTSWAEWGQRELRLGHLAPFTTLHKDQPLSDRRSELKRRLKAEKKLAEKEAKQKE LSEKQLNQTASAPNHTADNGVGAEEETLDPNQYYKIRSQAVQQLKVTGEDPYPHKFHVDISLTQFIQEYS HLQPGDHLTDVTLKVAGRIHAKRASGGKLIFYDLRGEGVKLQVMANSRNYKSEEEFVHINNKLRRGDIIG VEGNPGKTKKGELSIIPQEITLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNDFVRQKFIVRSKIITY IRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFR NEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMLSGMVKSITGSYKITYHPDGPEGQAYEVDFTPPFRR ISMVEELEKALGVKLPETSLFETEETRKILDDICVAKAVECPPPRTTARLLDKLVGEFLEVTCISPTFIC DHPQIMSPLAKWHRSKEGLTERFELFVMKKEICNAYTELNDPVRQRQLFEEQAKAKAAGDDEAMFIDENF CTALEYGLPPTAGWGMGIDRLTMFLTDSNNIKEVLLFPAMKPEDKKETAATTETPESTEASPSV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC027356 |
| ORF Size | 1872 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC027356.1 |
| RefSeq Size | 2205 bp |
| RefSeq ORF | 1874 bp |
| Locus ID | 85305 |
| MW | 80.9 kDa |
| Gene Summary | Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218510 | Kars (untagged) - Mouse lysyl-tRNA synthetase (cDNA clone MGC:37041 IMAGE:4950333), (10ug) |
CNY 6940.00 |
|
| MG209528 | Kars (tGFP-tagged) - Mouse lysyl-tRNA synthetase (cDNA clone MGC:37041 IMAGE:4950333) |
CNY 6940.00 |
|
| MR209528L3 | Lenti ORF clone of Kars (Myc-DDK-tagged) - Mouse lysyl-tRNA synthetase (cDNA clone MGC:37041 IMAGE:4950333) |
CNY 8170.00 |
|
| MR209528L4 | Lenti ORF clone of Kars (mGFP-tagged) - Mouse lysyl-tRNA synthetase (cDNA clone MGC:37041 IMAGE:4950333) |
CNY 8170.00 |
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
