United States
Germany
Japan
United Kingdom
China
Cdyl (NM_009881) Mouse Tagged ORF Clone
CAT＃: MR209233
- TrueORF®
Cdyl (Myc-DDK-tagged) - Mouse chromodomain protein, Y chromosome-like (Cdyl), transcript variant 1
ORF Plasmid: tGFP
"NM_009881" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,890.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI325931 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209233 representing NM_009881
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCATAGGCAATAGCCAGCCTAATTCACAGGAAGCCCAGCTCTGCACACTTCCAGAGAAAGCTGAAC AACCTACTGATGATAACACCTGCCAGCAAAATAATGTGGTTCCTGCAACAGTCTCAGAACCCGATCAAGC GTCCCCTGCAATTCAAGACGCGGAGACTCAGGTGGAAAGTATCGTTGACAAAAGGAAAAACAAGAAAGGG AAGACAGAATATCTGGTGCGGTGGAAAGGCTATGACAGTGAGGATGACACGTGGGAGCCTGAGCAGCACC TGGTGAACTGTGAGGAATACATCCATGACTTCAACCGGCGCCACAACGAGAGGCAAAAGGAAGGTAGCCT GGCTCGTGCCAGCAGAGCCTCCCCCAGCAACGCCCGGAAGCAGATTTCCAGGTCCACCCACAGCACTCTC TCCAAGACCAACTCCAAAGCACTTGTGGTAGGCAAAGATCATGAGTCCAAAAGCAGCCAGCTGTTGGCTG CCAGCCAGAAGTTCAGGAAAAACCCAGCCCCATCTCTTGCAAACCGCAAGAACATGGACCTCGCCAAGTC AGGGATCAAAATTCTCGTGCCTAAGAGCCCCGTTAAGGGCAGGACCTCGGTTGATGGCTTTCAGGGGGAG AGCCCCGAGAAGCTGGACCCTGTGGATCAGGGTGCCGAGGACACTGTAGCCCCAGAGGTGACTGCAGAGA AGCCCACTGGGGCTTTGCTGGGCCCTGGTGCGGAGCGAGCCAGGATGGGGAGCAGGCCCCGAATACATCC ACTAGTGCCTCAGGTTTCTGGCCCCGTGACTGCTGCCATGGCCACAGGCTTAGCTGTTAATGGAAAAGGT ACATCTCCATTCATGGATGCGCTAGCAGCCAACGGAACAGTCACCATACAGACATCCGTAACAGGAGTGA CAGCCGGGAAAAGGAAATTTATTGACGACAGAAGAGACCAACCTTTTGACAAGCGGTTGCGTTTCAGTGT GAGGCAGACAGAGAGTGCCTACAGATACAGAGATATTGTCGTCAGGAAGCAAGATGGCTTCACCCACATC TTGTTATCCACAAAATCGTCAGAGAATAACTCACTAAACCCAGAGGTGATGAAAGAAGTGCAGAGCGCCC TGAGCACAGCTGCAGCCGACGACAGCAAGCTGGTTCTGCTCAGCGCCGTGGGCAGCGTCTTCTGCTGTGG TCTGGACTTTATTTATTTTATTCGGCGCCTCACAGATGACCGAAAGAGAGAAAGCACTAAAATGGCAGAC GCTATCAGAAACTTCGTGAATACTTTCATTCAGTTTAAGAAGCCTATTATTGTAGCTGTTAATGGCCCAG CCATTGGACTAGGAGCATCCATATTGCCTCTTTGTGATGTGGTTTGGGCTAACGAAAAGGCTTGGTTTCA AACACCCTATACCACCTTCGGACAGAGTCCAGATGGCTGCTCTACCGTTATGTTTCCCAAGATTATGGGA GGAGCATCTGCGAATGAAATGCTGTTCAGTGGGCGGAAGTTGACGGCACAGGAGGCCTGTGGCAAGGGTC TGGTCTCCCAGGTGTTTTGGCCAGGAACCTTCACACAGGAAGTCATGGTTCGAATCAAGGAGCTGGCTTC ATGTAACCCAGTTGTCCTGGAGGAATCCAAAGCCCTGGTGCGCTGCAATATGAAGATGGAGCTAGAGCAG GCCAATGAGAGAGAATGTGAAGTGCTGAAGAAGATCTGGGGCTCCGCCCAGGGCATGGACTCCATGTTAA AGTACTTACAGAGGAAAATCGATGAGTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209233 representing NM_009881
Red=Cloning site Green=Tags(s) MGIGNSQPNSQEAQLCTLPEKAEQPTDDNTCQQNNVVPATVSEPDQASPAIQDAETQVESIVDKRKNKKG KTEYLVRWKGYDSEDDTWEPEQHLVNCEEYIHDFNRRHNERQKEGSLARASRASPSNARKQISRSTHSTL SKTNSKALVVGKDHESKSSQLLAASQKFRKNPAPSLANRKNMDLAKSGIKILVPKSPVKGRTSVDGFQGE SPEKLDPVDQGAEDTVAPEVTAEKPTGALLGPGAERARMGSRPRIHPLVPQVSGPVTAAMATGLAVNGKG TSPFMDALAANGTVTIQTSVTGVTAGKRKFIDDRRDQPFDKRLRFSVRQTESAYRYRDIVVRKQDGFTHI LLSTKSSENNSLNPEVMKEVQSALSTAAADDSKLVLLSAVGSVFCCGLDFIYFIRRLTDDRKRESTKMAD AIRNFVNTFIQFKKPIIVAVNGPAIGLGASILPLCDVVWANEKAWFQTPYTTFGQSPDGCSTVMFPKIMG GASANEMLFSGRKLTAQEACGKGLVSQVFWPGTFTQEVMVRIKELASCNPVVLEESKALVRCNMKMELEQ ANERECEVLKKIWGSAQGMDSMLKYLQRKIDEF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009881 |
| ORF Size | 1779 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009881.3, NP_034011.1 |
| RefSeq Size | 3444 bp |
| RefSeq ORF | 1782 bp |
| Locus ID | 12593 |
| UniProt ID | Q9WTK2 |
| MW | 65.7 kDa |
| Gene Summary | Isoform 2: Chromatin reader protein that recognizes and binds histone H3 trimethylated at 'Lys-9', dimethylated at 'Lys-27' and trimethylated at 'Lys-27' (H3K9me3, H3K27me2 and H3K27me3, respectively) (PubMed:12947414). Part of multimeric repressive chromatin complexes, where it is required for transmission and restoration of repressive histone marks, thereby preserving the epigenetic landscape (PubMed:12947414). Required for chromatin targeting and maximal enzymatic activity of Polycomb repressive complex 2 (PRC2); acts as a positive regulator of PRC2 activity by bridging the pre-existing histone H3K27me3 and newly recruited PRC2 on neighboring nucleosomes (By similarity). Acts as a corepressor for REST by facilitating histone-lysine N-methyltransferase EHMT2 recruitment and H3K9 dimethylation at REST target genes for repression (By similarity). Involved X chromosome inactivation in females: recruited to Xist RNA-coated X chromosome and facilitates propagation of H3K9me2 by anchoring EHMT2 (PubMed:24144980). Required for neuronal migration during brain development by repressing expression of RHOA (PubMed:28076783). In addition to act as a chromatin reader, acts as a hydro-lyase (By similarity). Shows crotonyl-coA hydratase activity by mediating the conversion of crotonyl-CoA ((2E)-butenoyl-CoA) to beta-hydroxybutyryl-CoA (3-hydroxybutanoyl-CoA), thereby acting as a negative regulator of histone crotonylation (By similarity). Histone crotonylation is required during spermatogenesis; down-regulation of histone crotonylation by CDYL regulates the reactivation of sex chromosome-linked genes in round spermatids and histone replacement in elongating spermatids (PubMed:28803779). May have histone acetyltransferase activity; such activity is however unsure in vivo (PubMed:12072557).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205145 | Cdyl (untagged) - Mouse chromodomain protein, Y chromosome-like (Cdyl), transcript variant 1, (10ug) |
CNY 2,000.00 |
|
| MG209233 | Cdyl (tGFP-tagged) - Mouse chromodomain protein, Y chromosome-like (Cdyl) |
CNY 6,460.00 |
|
| MR209233L3 | Lenti ORF clone of Cdyl (Myc-DDK-tagged) - Mouse chromodomain protein, Y chromosome-like (Cdyl), transcript variant 1 |
CNY 7,790.00 |
|
| MR209233L4 | Lenti ORF clone of Cdyl (mGFP-tagged) - Mouse chromodomain protein, Y chromosome-like (Cdyl), transcript variant 1 |
CNY 7,790.00 |
