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Me2 (NM_145494) Mouse Tagged ORF Clone
CAT＃: MR209185
- TrueORF®
Me2 (Myc-DDK-tagged) - Mouse malic enzyme 2, NAD(+)-dependent, mitochondrial (Me2), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_145494" in other vectors (4)
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CNY 4,392.00
CNY 5,230.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW120568; D030040L20Rik; NAD-ME |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209185 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTTCCCGGCTAAGAGCTGTTACCACTCCTTGTACCTTGACCTGCCGCCGAGTGCACCTGAAAGAGA AGGGCAAGCCACTCATGTTGAATCCAAGAACAAACAAGGGAATGGCGTTTACGTTACAAGAGCGACAAAT GCTTGGTCTTCAAGGACTTTTACCTCCTAAAATAGAGACACAGGATATTCAAGCCTTACGATTCCATAGA AACTTGAAAAAAATGACTAGCCCTTTGGAAAAATACATTTATATAATGGGCATCCAAGAAAGAAATGAGA AACTGTTCTATAGAATCCTGCAGGATGATATTGAAAGTCTGATGCCGATTGTGTACACGCCAACAGTTGG TCTTGCCTGCTGTCAATATGGGCACATCTTTAGGAGACCTAAGGGATTGTTTATTTCAATCTCAGACAGA GGTCATGTTAGATCAATTGTGGATAACTGGCCAGAAAATCATGTTAAGGCTGTTGTGGTGACTGATGGAG AGAGGATCCTGGGCCTTGGCGATCTGGGTGTCTATGGAATGGGGATTCCAGTAGGGAAGCTGTGCCTGTA TACAGCCTGTGCAGGGATACAGCCCGAGAAGTGCCTGCCCGTGTGCATCGACGTGGGCACTGATAACATG GCACTATTAAAAGATCCGTTTTATATGGGCTTGTATCAGAAGCGAGATCGTTCCCAGCTCTATGATGATC TGATGGACGAGTTTATGAAGGCGATTACTGACAGATACGGCCGGAACACACTCATTCAGTTTGAAGACTT TGGAAATCATAATGCATTCAGGTTCTTAAGAAAATATCAACAAAAATACTGTACCTTCAATGATGACATT CAAGGGACAGCTGCAGTTGCTCTGTCTGGTCTTCTTGCGGCCCAAAGAGTTATTAATAAACCAGTCTCAG AACACAAAATCTTATTTCTTGGAGCAGGAGAAGCTGCACTTGGAATTGCCAATCTCATAGTTCTATCAAT GGTGGAAAGTGGTCTCTCAGAAGAGGAGGCGCAGAGAAAGATCTGGATGTTTGACAAGAGTGGCTTGCTG GTTAAGGGGCGGACTGCTAGCATAGATAGCAACCAGGAACCATATGCCCATGCGGCCCCAGAGAGCATAC CTGCGACTTTTGAAGATGCAGTAAATAAACTTAAGCCTTCAGTTATAATTGGAGTGGCAGGTGCCGGCCC GCTTTTTACTCACGGTGTAATCAAAGCCATGGCCTCTATCAACGAGAGACCTATAATATTTGCTCTAAGT AATCCTACAGCACAGGCCGAGTGCACAGCCGAGGACGCGTACACGCTCACAGAGGGCAGGTGTCTGTTTG CCAGTGGCAGCCCATTTGAGCCAGTGAAACTCCAGGATGGACGAGTCTTCACCCCAGGCCAAGGAAACAA TGCATATATTTTCCCAGGTGTGGCTTTAGCTGTTATTCTCTGTGAAGCCCGACACATCAGTGACACCGTT TTCTTAGAAGCTGCAAAGGCGCTGACGACTCAGTTGACAGATGCAGAGTTAGCCCAAGGGAGGCTTTACC CGTCGCTGGCTAATATTCAGGAAGTTTCTGCTAACATAGCTATTAAACTTGCAGAGTACCTGTACGCTAA TAAAATGGCCTTCCGGTACCCAGAACCCGAGGACAAGGCCAGATACGTGAGAGAGAGAATATGGCGGAGC AACTACGTTTCCCTGCTGCCGGACGTGTATGACTGGCCAGAGTCTTCACTGACGCCTCCTCAGATCACAG AAGAGAAGCTTCCCCAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR209185 protein sequence
Red=Cloning site Green=Tags(s) MFSRLRAVTTPCTLTCRRVHLKEKGKPLMLNPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFHR NLKKMTSPLEKYIYIMGIQERNEKLFYRILQDDIESLMPIVYTPTVGLACCQYGHIFRRPKGLFISISDR GHVRSIVDNWPENHVKAVVVTDGERILGLGDLGVYGMGIPVGKLCLYTACAGIQPEKCLPVCIDVGTDNM ALLKDPFYMGLYQKRDRSQLYDDLMDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYQQKYCTFNDDI QGTAAVALSGLLAAQRVINKPVSEHKILFLGAGEAALGIANLIVLSMVESGLSEEEAQRKIWMFDKSGLL VKGRTASIDSNQEPYAHAAPESIPATFEDAVNKLKPSVIIGVAGAGPLFTHGVIKAMASINERPIIFALS NPTAQAECTAEDAYTLTEGRCLFASGSPFEPVKLQDGRVFTPGQGNNAYIFPGVALAVILCEARHISDTV FLEAAKALTTQLTDAELAQGRLYPSLANIQEVSANIAIKLAEYLYANKMAFRYPEPEDKARYVRERIWRS NYVSLLPDVYDWPESSLTPPQITEEKLPH SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145494 |
| ORF Size | 1770 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145494.1 |
| RefSeq Size | 2455 bp |
| RefSeq ORF | 1770 bp |
| Locus ID | 107029 |
| UniProt ID | Q99KE1 |
| MW | 65.8 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205582 | Me2 (untagged) - Mouse malic enzyme 2, NAD(+)-dependent, mitochondrial (Me2), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 2,000.00 |
|
| MG209185 | Me2 (tGFP-tagged) - Mouse malic enzyme 2, NAD(+)-dependent, mitochondrial (Me2) |
CNY 5,800.00 |
|
| MR209185L3 | Lenti ORF clone of Me2 (Myc-DDK-tagged) - Mouse malic enzyme 2, NAD(+)-dependent, mitochondrial (Me2), nuclear gene encoding mitochondrial protein |
CNY 7,130.00 |
|
| MR209185L4 | Lenti ORF clone of Me2 (mGFP-tagged) - Mouse malic enzyme 2, NAD(+)-dependent, mitochondrial (Me2), nuclear gene encoding mitochondrial protein |
CNY 7,130.00 |
