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Pkm (NM_011099) Mouse Tagged ORF Clone
CAT＃: MR208518
- TrueORF®
Pkm (Myc-DDK-tagged) - Mouse pyruvate kinase, muscle (Pkm2)
ORF Plasmid: tGFP
"NM_011099" in other vectors (4)
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CNY 5,944.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA414905; AL024370; AL024424; Pk-2; Pk-3; Pk3; Pkm2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208518 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGAAGCCACACAGTGAAGCAGGGACTGCCTTCATTCAGACCCAGCAGCTCCATGCAGCCATGGCTG ACACCTTCCTGGAACACATGTGCCGCCTGGACATTGACTCTGCCCCCATCACGGCCCGCAACACTGGCAT CATTTGTACCATTGGGCCTGCTTCCCGATCTGTGGAGATGCTGAAGGAGATGATTAAGTCTGGAATGAAT GTGGCTCGGCTGAATTTCTCTCATGGAACCCATGAGTACCATGCAGAGACCATCAAGAATGTCCGTGAAG CCACAGAAAGCTTTGCATCTGATCCCATTCTCTACCGTCCTGTTGCGGTGGCTCTGGATACAAAGGGACC TGAGATCCGGACTGGACTCATCAAGGGCAGCGGCACCGCTGAGGTGGAGCTGAAGAAGGGAGCCACTCTG AAGATCACCCTGGACAACGCTTACATGGAGAAGTGTGACGAGAACATCCTGTGGCTGGACTACAAGAACA TCTGCAAGGTGGTGGAGGTGGGCAGCAAGATCTACGTGGACGATGGGCTCATCTCACTGCAGGTGAAGGA GAAAGGCGCTGACTTCCTGGTGACGGAGGTGGAGAATGGTGGCTCCTTGGGCAGCAAGAAGGGCGTGAAC CTGCCGGGCGCTGCTGTGGATCTCCCCGCTGTGTCGGAAAAGGACATCCAGGACCTGAAGTTTGGGGTGG AGCAGGATGTGGACATGGTGTTTGCATCTTTCATCCGCAAGGCAGCCGACGTGCATGAAGTCAGGAAGGT GCTGGGAGAGAAGGGCAAGAACATCAAGATCATCAGCAAAATCGAGAACCATGAAGGCGTCCGCAGGTTT GATGAGATCTTGGAGGCCAGTGATGGGATCATGGTGGCTCGTGGTGACCTGGGCATTGAGATTCCTGCAG AGAAGGTCTTCCTGGCTCAGAAGATGATGATCGGGCGATGCAACCGAGCTGGGAAGCCTGTCATCTGTGC CACACAGATGCTGGAGAGCATGATCAAGAAGCCACGCCCCACCCGTGCTGAAGGCAGTGATGTGGCCAAT GCAGTCCTGGATGGAGCAGACTGCATCATGCTGTCTGGAGAAACAGCCAAGGGGGACTACCCTCTGGAGG CTGTTCGCATGCAGCACCTGATTGCCCGAGAGGCAGAGGCTGCCATCTACCACTTGCAGCTATTCGAGGA ACTCCGCCGCCTGGCGCCCATTACCAGCGACCCCACAGAAGCTGCCGCCGTGGGTGCCGTGGAGGCCTCC TTCAAGTGCTGCAGTGGGGCCATTATCGTGCTCACCAAGTCTGGCAGGAGTGCTCACCAAGTGGCCAGGT ACCGCCCTCGGGCTCCTATCATTGCCGTGACTCGAAATCCCCAGACTGCTCGCCAGGCCCATCTGTACCG TGGCATCTTCCCTGTGCTGTGTAAGGATGCCGTGCTGAATGCCTGGGCTGAGGATGTCGACCTTCGTGTA AACTTGGCCATGGATGTTGGCAAGGCCCGAGGCTTCTTCAAGAAGGGAGATGTGGTCATTGTGCTGACCG GGTGGCGCCCTGGCTCTGGATTCACCAACACCATGCGTGTAGTGCCTGTACCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208518 protein sequence
Red=Cloning site Green=Tags(s) MPKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSAPITARNTGIICTIGPASRSVEMLKEMIKSGMN VARLNFSHGTHEYHAETIKNVREATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATL KITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKEKGADFLVTEVENGGSLGSKKGVN LPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEKGKNIKIISKIENHEGVRRF DEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVAN AVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEAAAVGAVEAS FKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDAVLNAWAEDVDLRV NLAMDVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011099 |
| ORF Size | 1596 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011099.4 |
| RefSeq Size | 2432 bp |
| RefSeq ORF | 1596 bp |
| Locus ID | 18746 |
| UniProt ID | P52480 |
| MW | 57.8 kDa |
| Gene Summary | Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP (By similarity). Stimulates POU5F1-mediated transcriptional activation (By similarity). Promotes in a STAT1-dependent manner, the expression of the immune checkpoint protein CD274 in ARNTL/BMAL1-deficient macrophages (PubMed:29996098).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203724 | Pkm (untagged) - Mouse pyruvate kinase, muscle (Pkm2), (10ug) |
CNY 5,944.00 |
|
| MG208518 | Pkm (tGFP-tagged) - Mouse pyruvate kinase, muscle (Pkm2) |
CNY 7,544.00 |
|
| MR208518L3 | Lenti ORF clone of Pkm (Myc-DDK-tagged) - Mouse pyruvate kinase, muscle (Pkm2) |
CNY 8,344.00 |
|
| MR208518L4 | Lenti ORF clone of Pkm (mGFP-tagged) - Mouse pyruvate kinase, muscle (Pkm2) |
CNY 8,344.00 |
