Shoc2 (BC026364) Mouse Tagged ORF Clone
CAT＃: MR208396
- TrueORF®
Shoc2 (Myc-DDK-tagged) - Mouse soc-2 (suppressor of clear) homolog (C. elegans) (cDNA clone MGC:31051 IMAGE:4014650)
ORF Plasmid: tGFP
"BC026364" in other vectors (3)
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CNY 3904.00
Product images
CNY 6840.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Sur8, Sur-8 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208396 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTAGTAGTTTAGGAAAAGAAAAAGACTCTAAAGAGAAAGACCCCAAAGTACCGTCTGCCAAGGAAA GAGAAAAGGAGTCAAAAGCCTCAGGAGGCTTTGGGAAAGAGAGCAAAGAAAAGGAACCTAAAGCCAAAGG GAGAGACGCCAAAGATGGAAAGAAGGAGTCCAGCGCTGCCCAGCCAGGTGTGGCTTTTTCAGTCGACAAT ACCATCAAACGGCCAAATCCAGCACCCGGCACTAGAAAAAAGTCCAGCAATGCTGAGGTCATTAAGGAGC TTAACAAATGCCGGGAGGAGAACTCAATGCGGTTGGACTTGTCCAAGAGGTCTATACATATACTGCCACC ATCAGTCAAAGAGTTGACTCAACTCACAGAACTTTATTTATACAGTAACAAATTGCAGTCCCTCCCAGCA GAGGTGGGCTGTCTAGTCAATCTCATGACGCTGGCTCTCAGTGAGAATTCACTCACCAGTTTGCCTGACT CTCTTGATAACTTGAAGAAGCTGCGGATGCTTGATTTACGGCATAATAAACTGAGAGAAATTCCTTCAGT GGTGTATAGGCTAGACTCTCTCACCACTCTCTATCTTCGCTTTAATCGCATAACTACTGTGGAAAAGGAC ATCAAAAACCTGCCGAAGCTCAGCATGCTCAGCATCCGAGAGAACAAAATCAAGCAGCTGCCTGCTGAAA TTGGTGAATTATGTAACCTCATTACCCTGGATGTAGCTCACAATCAACTTGAACACCTTCCAAAGGAGAT TGGAAACTGCACACAGATAACCAACCTTGACTTGCAGCACAATGACCTACTGGATCTCCCAGATACAATA GGAAACCTGTCCAGTTTAAATCGCCTTGGCCTGAGATACAATAGATTGTCAGCAATACCCAGATCATTAG CAAAATGCAGTGCACTTGAGGAGTTAAATTTAGAGAACAATAACATTTCTACTCTACCAGAGAGTCTTTT ATCCAGTCTTGTAAAATTGAATAGCTTGACCTTAGCTAGAAATTGCTTCCAGTTATATCCAGTGGGAGGT CCATCTCAGTTTTCCACCATTTATTCCCTCAACATGGAACACAATCGAATCAACAAAATCCCATTTGGAA TTTTTTCCAGAGCTAAAGTGTTAAGTAAGCTGAATATGAAGGACAATCAGTTAACATCACTTCCTTTGGA TTTTGGAACTTGGACCAGTATGGTAGAATTGAATTTAGCGACTAATCAGCTCACAAAGATCCCAGAGGAT GTGTCTGGTCTCGTTTCCCTTGAGGTTCTCATCTTATCGAACAACCTTCTAAAGAAGCTGCCCCACGGCC TTGGGAACCTCAGAAAGCTACGAGAGCTGGACCTGGAGGAGAACAAGCTGGAGTCCTTGCCCAATGAGAT CGCGTATCTCAAGGATCTGCAGAAATTAGTCTTGACAAACAACCAGTTGAGCACGCTTCCCAGAGGCATC GGTCACCTTACCAACCTCACGCACCTCGGTCTTGGAGAGAACCTGCTCACTCACCTTCCTGAGGAAATCG GTAGGAACCTGAGGGCGCGGCGCACTGCTGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208396 protein sequence
Red=Cloning site Green=Tags(s) MSSSLGKEKDSKEKDPKVPSAKEREKESKASGGFGKESKEKEPKAKGRDAKDGKKESSAAQPGVAFSVDN TIKRPNPAPGTRKKSSNAEVIKELNKCREENSMRLDLSKRSIHILPPSVKELTQLTELYLYSNKLQSLPA EVGCLVNLMTLALSENSLTSLPDSLDNLKKLRMLDLRHNKLREIPSVVYRLDSLTTLYLRFNRITTVEKD IKNLPKLSMLSIRENKIKQLPAEIGELCNLITLDVAHNQLEHLPKEIGNCTQITNLDLQHNDLLDLPDTI GNLSSLNRLGLRYNRLSAIPRSLAKCSALEELNLENNNISTLPESLLSSLVKLNSLTLARNCFQLYPVGG PSQFSTIYSLNMEHNRINKIPFGIFSRAKVLSKLNMKDNQLTSLPLDFGTWTSMVELNLATNQLTKIPED VSGLVSLEVLILSNNLLKKLPHGLGNLRKLRELDLEENKLESLPNEIAYLKDLQKLVLTNNQLSTLPRGI GHLTNLTHLGLGENLLTHLPEEIGRNLRARRTAG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC026364 |
| ORF Size | 1572 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC026364, AAH26364 |
| RefSeq Size | 2025 bp |
| RefSeq ORF | 1574 bp |
| Locus ID | 56392 |
| MW | 58.5 kDa |
| Gene Summary | Regulatory subunit of protein phosphatase 1 (PP1c) that acts as a M-Ras/MRAS effector and participates in MAPK pathway activation. Upon M-Ras/MRAS activation, targets PP1c to specifically dephosphorylate the 'Ser-259' inhibitory site of RAF1 kinase and stimulate RAF1 activity at specialized signaling complexes.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG208396 | Shoc2 (tGFP-tagged) - Mouse soc-2 (suppressor of clear) homolog (C. elegans) (cDNA clone MGC:31051 IMAGE:4014650) |
CNY 3230.00 |
|
| MR208396L3 | Lenti ORF clone of Shoc2 (Myc-DDK-tagged) - Mouse soc-2 (suppressor of clear) homolog (C. elegans) (cDNA clone MGC:31051 IMAGE:4014650) |
CNY 4850.00 |
|
| MR208396L4 | Lenti ORF clone of Shoc2 (mGFP-tagged) - Mouse soc-2 (suppressor of clear) homolog (C. elegans) (cDNA clone MGC:31051 IMAGE:4014650) |
CNY 4850.00 |
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
