Cdc25a (NM_007658) Mouse Tagged Lenti ORF Clone
CAT＃: MR208257L3
- LentiORF®
Lenti ORF clone of Cdc25a (Myc-DDK-tagged) - Mouse cell division cycle 25 homolog A (S. pombe) (Cdc25a)
"NM_007658" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 7030.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | D9Ertd393e |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR208257).
|
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NM_007658 |
| ORF Size | 1542 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_007658.3 |
| RefSeq Size | 3643 bp |
| RefSeq ORF | 1545 bp |
| Locus ID | 12530 |
| UniProt ID | P48964 |
| Gene Summary | Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro (By similarity). Phosphorylation by PIM1 leads to an increase in phosphatase activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204476 | Cdc25a (untagged) - Mouse cell division cycle 25 homolog A (S. pombe) (Cdc25a), (10ug) |
CNY 3832.00 |
|
| MG208257 | Cdc25a (tGFP-tagged) - Mouse cell division cycle 25 homolog A (S. pombe) (Cdc25a) |
CNY 5610.00 |
|
| MR208257 | Cdc25a (Myc-DDK-tagged) - Mouse cell division cycle 25 homolog A (S. pombe) (Cdc25a) |
CNY 5130.00 |
|
| MR208257L4 | Lenti ORF clone of Cdc25a (mGFP-tagged) - Mouse cell division cycle 25 homolog A (S. pombe) (Cdc25a) |
CNY 7030.00 |
