Rgs7 (BC051133) Mouse Tagged ORF Clone
CAT＃: MR207517
- TrueORF®
Rgs7 (Myc-DDK-tagged) - Mouse regulator of G protein signaling 7 (cDNA clone MGC:58271 IMAGE:6588901)
ORF Plasmid: tGFP
"BC051133" in other vectors (4)
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CNY 3656.00
Product images
CNY 6840.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207517 representing BC051133
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAGGGAAATAATTATGGACAGACCAGCAACGGGGTGGCGGACGAATCACCCAACATGCTGGTGT ACAGAAAGATGGAAGATGTCATAGCACGTATGCAAGACGAGAAAAACGGAATTCCCATCCGTACCGTCAA AAGCTTCCTTTCCAAGATCCCCAGTGTGTTCTCCGGGTCAGACATTGTTCAATGGTTAATAAAGAACTTA ACTATAGAAGATCCAGTGGAGGCTCTCCATTTGGGAACGCTAATGGCTGCCCATGGCTACTTCTTTCCCA TCTCAGATCATGTCCTCACACTCAAGGATGACGGCACCTTCTACCGGTTTCAAACACCCTATTTTTGGCC GTCAAATTGTTGGGAGCCAGAAAACACAGACTATGCTGTTTACCTCTGCAAGAGAACAATGCAAAACAAG GCAAGGCTAGAGCTAGCAGATTATGAAGCCGAGAGCCTGGCCAGGCTGCAGAGAGCATTTGCCCGGAAGT GGGAGTTCATTTTTATGCAAGCAGAAGCACAAGCTAAAGTGGACAAGAAGAGAGACAAAATTGAAAGGAA GATCCTCGATAGTCAAGAGAGAGCATTCTGGGATGTCCACAGGCCTGTGCCTGGATGTGTAAATACTACA GAAGTGGACATTAAGAAGTCATCCCGGATGAGAAACCCACACAAAACACGAAAGTCTGTCTATGGTTTAC AAAATGACATCCGAAGTCACAGTCCCACCCACACACCTACACCAGAAACCAAGCCTCCTACAGAAGATGA GCTGCACCAACAGATAAAATACTGGCAAATACAGTTAGATAGACATCGGTTAAAAATGTCAAAAGTTGCT GATAGTCTACTAAGCTATACGGAACAGTATGTAGAATATGACCCGTTTCTTGTGCCGCCTGACCCTTCCA ATCCATGGCTCTCAGATGATACGACTTTCTGGGAACTTGAAGCAAGCAAAGAACCAAGTCAACAGAGGGT AAAACGATGGGGTTTTGGTATGGATGAGGCATTGAAAGACCCAGTTGGGCGAGAGCAGTTCCTTAAGTTT CTAGAATCAGAATTCAGCTCAGAGAACTTAAGGTTCTGGTTGGCAGTGGAGGACCTGAAGAGAAGGCCTA TCCGAGAGGTCCCCTCGAGAGTGCAGGAAATATGGCAAGAATTTCTGGCTCCTGGGGCCCCCAGTGCCAT TAACTTGGATTCTAAGAGTTATGACAAGACCACACAGAATGTGAAGGAACCAGGACGATACACATTTGAA GATGCTCAGGAGCATATTTACAAGCTGATGAAGAGCGACTCATACCCCCGCTTTATAAGATCTAGTGCCT ACCAGGAACTTCTACAGGCAAAGAGAAAGAGATGCCATGAAAGGCTGCTCCACGCCGTACTTAGCCCAAG AGCCTTTGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207517 representing BC051133
Red=Cloning site Green=Tags(s) MAQGNNYGQTSNGVADESPNMLVYRKMEDVIARMQDEKNGIPIRTVKSFLSKIPSVFSGSDIVQWLIKNL TIEDPVEALHLGTLMAAHGYFFPISDHVLTLKDDGTFYRFQTPYFWPSNCWEPENTDYAVYLCKRTMQNK ARLELADYEAESLARLQRAFARKWEFIFMQAEAQAKVDKKRDKIERKILDSQERAFWDVHRPVPGCVNTT EVDIKKSSRMRNPHKTRKSVYGLQNDIRSHSPTHTPTPETKPPTEDELHQQIKYWQIQLDRHRLKMSKVA DSLLSYTEQYVEYDPFLVPPDPSNPWLSDDTTFWELEASKEPSQQRVKRWGFGMDEALKDPVGREQFLKF LESEFSSENLRFWLAVEDLKRRPIREVPSRVQEIWQEFLAPGAPSAINLDSKSYDKTTQNVKEPGRYTFE DAQEHIYKLMKSDSYPRFIRSSAYQELLQAKRKRCHERLLHAVLSPRAFV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC051133 |
| ORF Size | 1410 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC051133.1 |
| RefSeq Size | 2462 bp |
| RefSeq ORF | 1412 bp |
| Locus ID | 24012 |
| MW | 55 kDa |
| Gene Summary | Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. The RGS7/GNB5 dimer enhances GNAO1 GTPase activity. May play a role in synaptic vesicle exocytosis. Modulates the activity of potassium channels that are activated by GNAO1 in response to muscarinic acetylcholine receptor M2/CHRM2 signaling.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218280 | Rgs7 (untagged) - Mouse regulator of G protein signaling 7 (cDNA clone MGC:58271 IMAGE:6588901), (10ug) |
CNY 6940.00 |
|
| MG207517 | Rgs7 (tGFP-tagged) - Mouse regulator of G protein signaling 7 (cDNA clone MGC:58271 IMAGE:6588901) |
CNY 2950.00 |
|
| MR207517L3 | Lenti ORF clone of Rgs7 (Myc-DDK-tagged) - Mouse regulator of G protein signaling 7 (cDNA clone MGC:58271 IMAGE:6588901) |
CNY 4560.00 |
|
| MR207517L4 | Lenti ORF clone of Rgs7 (mGFP-tagged) - Mouse regulator of G protein signaling 7 (cDNA clone MGC:58271 IMAGE:6588901) |
CNY 4560.00 |
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
