Oc90 (BC048716) Mouse Tagged ORF Clone
CAT＃: MR207235
- TrueORF®
Oc90 (Myc-DDK-tagged) - Mouse otoconin 90 (cDNA clone MGC:59513 IMAGE:6334417)
ORF Plasmid: tGFP
"BC048716" in other vectors (4)
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CNY 3656.00
Product images
CNY 300.00
CNY 6840.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PLA2L, Ocn-95 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207235 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTATGCTGCTCATGGTCGGTATGCTGATGGCCCCCTGTGTTGGGGCCCATGCTCTGGACACACCAA ATCCCCAGGAATTGCCTCCAGGACTGTCAAAAAATATAAATATCACTTTCTTCAATGGAGTGTTTAAAAA CGTGGAAAGTGTGGCTGAAATTTTTGACTGCCTAGGTTCCCACTTCACCTGGCTGCAGGCTGTCTTCACC AACTTCCCGCTGCTCCTCCAGTTTGTAAACAGTATGAGGTGTGTAACTGGCCTCTGCCCCCGGGACTTTG AAGACTATGGTTGTGCCTGTAGGTTTGAGATGGAAGGGATGCCTGTGGACGAGTCTGATATCTGCTGCTT CCAGCATCGCAGGTGCTATGAGGAAGCTGTTGAGATGGACTGTCTCCAAGACCCTGCCAAGCTCAGTGCA GATGTGGATTGCACCAACAAACAGATCACATGTGAGTCCGAGGATCCCTGTGAGCGTCTACTGTGTACGT GTGACAAGGCTGCTGTGGAGTGCCTGGCTCAGTCTGGCATCAACTCCTCCCTGAACTTCCTGGATGCTTC CTTCTGCCTCCCTCAGACTCCAGAGACAACTAGTGGGAAGGCTGCAACACTGTTGCCTAGAGAGTCTGTT CAAGATCTTCAAGACACACAAGCTTCTAGGACCACATCAAGTCCAGGATCTGCAGAGATTATTGCCCTAG CCAAAGGTACAACCCACTCTGCTGGCATCAAACCACTGAGGTTGGGAGTCTCATCTGTTGACAATGGTTC CCAGGAAGCAGCTGGAAAAGCAGCCTGTGACAGATTGGCCTTCGTGCATCTGGGTGATGGGGACAGCATG ACGGCCATGCTGCAGCTTGGAGAGATGCTCTTCTGTCTAACATCCCATTGCCCAGAGGAATTTGAAACTT ACGGCTGCTACTGTGGAAGAGAAGGAAGAGGAGAGCCAAGGGACACCCTGGATAGGTGCTGTCTGTCCCA TCACTGCTGTTTGGAGCAGATGAGACAAGTGGGCTGCCTCCATGGAAGGCGTTCTCAGTCATCTGTGGTA TGTGAAGACCACATGGCCAAATGTGTGGGGCAGAGCCTGTGTGAGAAGCTACTATGTGCCTGTGACCAGA TGGCAGCTGAGTGCATGGCCTCTGCCTTCTTTAATCAAAGCCTCAAGTCACCAGACGGAGCCGAGTGTCA AGGCGAGCCTGTGTCCTGTGAGGATGGCATGCTCCAGGGCACCTTGGCCTCTTCTGTGGACTCCAGTTCT GAGGAGAATAGTGAGGAAGCTCCACCGCAGATGGAACGCCTAAGAAGATTTCTGGAAAAGCCTCCTGGTC CCTTGGGGGCCAGACCCCTCGGTGGGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207235 protein sequence
Red=Cloning site Green=Tags(s) MIMLLMVGMLMAPCVGAHALDTPNPQELPPGLSKNINITFFNGVFKNVESVAEIFDCLGSHFTWLQAVFT NFPLLLQFVNSMRCVTGLCPRDFEDYGCACRFEMEGMPVDESDICCFQHRRCYEEAVEMDCLQDPAKLSA DVDCTNKQITCESEDPCERLLCTCDKAAVECLAQSGINSSLNFLDASFCLPQTPETTSGKAATLLPRESV QDLQDTQASRTTSSPGSAEIIALAKGTTHSAGIKPLRLGVSSVDNGSQEAAGKAACDRLAFVHLGDGDSM TAMLQLGEMLFCLTSHCPEEFETYGCYCGREGRGEPRDTLDRCCLSHHCCLEQMRQVGCLHGRRSQSSVV CEDHMAKCVGQSLCEKLLCACDQMAAECMASAFFNQSLKSPDGAECQGEPVSCEDGMLQGTLASSVDSSS EENSEEAPPQMERLRRFLEKPPGPLGARPLGGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | BC048716 |
| ORF Size | 1359 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC048716, AAH48716 |
| RefSeq Size | 1701 bp |
| RefSeq ORF | 1361 bp |
| Locus ID | 18256 |
| MW | 49.1 kDa |
| Gene Summary | Major protein of the otoconia, a calcium carbonate structure in the saccule and utricle of the ear (PubMed:17300776). Together with OTOL1, acts as a scaffold for otoconia biomineralization: sequesters calcium and forms interconnecting fibrils between otoconia that are incorporated into the calcium crystal structure (PubMed:21655225, PubMed:24748133). Together with OTOL1, modulates calcite crystal morphology and growth kinetics (PubMed:24748133). It is unlikely that this protein has phospholipase A2 activity (PubMed:17300776).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218212 | Oc90 (untagged) - Mouse otoconin 90 (cDNA clone MGC:59513 IMAGE:6334417), (10ug) |
CNY 6940.00 |
|
| MG207235 | Oc90 (tGFP-tagged) - Mouse otoconin 90 (cDNA clone MGC:59513 IMAGE:6334417) |
CNY 2850.00 |
|
| MR207235L3 | Lenti ORF clone of Oc90 (Myc-DDK-tagged) - Mouse otoconin 90 (cDNA clone MGC:59513 IMAGE:6334417) |
CNY 4470.00 |
|
| MR207235L4 | Lenti ORF clone of Oc90 (mGFP-tagged) - Mouse otoconin 90 (cDNA clone MGC:59513 IMAGE:6334417) |
CNY 4470.00 |
