Frmd5 (BC057549) Mouse Tagged ORF Clone
CAT＃: MR206885
- TrueORF®
Frmd5 (Myc-DDK-tagged) - Mouse FERM domain containing 5 (cDNA clone MGC:66559 IMAGE:6416914)
ORF Plasmid: tGFP
"BC057549" in other vectors (3)
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CNY 3656.00
Product images
CNY 300.00
CNY 6840.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1500032A09Rik; 5830442F04; A930004K21Rik; R75430 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206885 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCTTCCGCGTGAAGTTTTACCCTGCCGACCCCGCTGCCCTGAAAGAGGAAATAACCAGGTACTTAG TCTTCCTTCAGATCAAAAGGGATCTCTACCACGGCCGTCTTCTCTGTAAAACATCGGATGCCGCCTTGTT AGCGGCCTATATCCTTCAAGCGGAGATTGGGGATTATGACCCGGGAAAGCACCCTGAAGGCTACAGCTCC AAGTTCCAATTTTTCCCGAAACATTCAGAGAAACTGGAAAAGAAAATTGCTGAGATTCACAAGACCGAAC TCAGTGGTCAAACACCAGCAACATCAGAGCTGAATTTCTTAAGAAAGGCCCAGACGTTAGAGACCTACGG AGTGGACCCTCACCCATGTAAGGATGTGTCGGGAAATGCTGCGTTTCTGGCCTTCACTCCTTTCGGGTTT GTTGTTCTTCAAGGAAACAAGAGGGTCCACTTCATTAAATGGAATGAGGTGACCAAGCTGAAATTTGAAG GAAAGACTTTCTATTTATATGTAAGTCAGAAAGAGGAAAAAAAGATTATTCTCACATATTTTGCCCCAAC TCCAGAAGCCTGCAAGCACCTCTGGAAATGTGGAATTGAGAACCAAGCCTTCTACAAGCTGGAAAAGTCA AGCCAAGTCCGCACAGTGTCCAGCAGCAATTTGTTCTTTAAGGGGAGCCGGTTCCGATACAGTGGCCGGG TCGCAAAGGAAGTAATGGAGTCCAGTGCCAAGATCAAACGGGAGCCGCCAGAAATACACAGAGCAGGGAT GGTCCCTAGCCGCAGCTGTCCTTCTATAACCCATGGCCCACGACTGAGCAGCGTCCCCAGGACACGAAGA AGAGCTGTGCACATCTCTATCATGGAAGGCCTTGAGTCGCTACGAGACAGTGCCCATTCCACCCCAGTGC GGTCCTCTTCCCATGGGGACACCTTCCTGCCTCACGTGAGAAGCAGCCGGGCAGACAGCAATGAACGTGT CGCTGTGATAGCGGATGAGGCCTACAGCCCCGCAGACAGTGTGCTGCCCACCCCTGTAGCCGAGCACAGC CTGGAGCTGATGCTGCTCTCCCGGCAGATCAACGGGGCCACGTGCAGCATTGAGGAGGAGAAGGAGTCGG AGGCCAGCACCCCGACTGCGACAGAGGCGGAGGCCCTGGGAGGAGAGCTGAGGGCCCTGTGTCAGGGGCA CGGCGGGTCAGAGCAAGAACAGATGCAAGAGAGAGACAGATCTGAGGAAGGCATCGAGAAGGAGCAGCGG CCTTGGAAGGGACCAGGAAGCACTAAGCAGGACCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206885 protein sequence
Red=Cloning site Green=Tags(s) MCFRVKFYPADPAALKEEITRYLVFLQIKRDLYHGRLLCKTSDAALLAAYILQAEIGDYDPGKHPEGYSS KFQFFPKHSEKLEKKIAEIHKTELSGQTPATSELNFLRKAQTLETYGVDPHPCKDVSGNAAFLAFTPFGF VVLQGNKRVHFIKWNEVTKLKFEGKTFYLYVSQKEEKKIILTYFAPTPEACKHLWKCGIENQAFYKLEKS SQVRTVSSSNLFFKGSRFRYSGRVAKEVMESSAKIKREPPEIHRAGMVPSRSCPSITHGPRLSSVPRTRR RAVHISIMEGLESLRDSAHSTPVRSSSHGDTFLPHVRSSRADSNERVAVIADEAYSPADSVLPTPVAEHS LELMLLSRQINGATCSIEEEKESEASTPTATEAEALGGELRALCQGHGGSEQEQMQERDRSEEGIEKEQR PWKGPGSTKQDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | BC057549 |
| ORF Size | 1296 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC057549, AAH57549 |
| RefSeq Size | 3370 bp |
| RefSeq ORF | 1298 bp |
| Locus ID | 228564 |
| MW | 48.5 kDa |
| Gene Summary | May be involved in regulation of cell migration. May regulate cell-matrix interactions via its interaction with ITGB5 and modifying ITGB5 cytoplasmic tail interactions such as with FERMT2 and TLN1. May regulate ROCK1 kinase activity possibly involved in regulation of actin stress fiber formation.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG206885 | Frmd5 (tGFP-tagged) - Mouse FERM domain containing 5 (cDNA clone MGC:66559 IMAGE:6416914) |
CNY 2760.00 |
|
| MR206885L3 | Lenti ORF clone of Frmd5 (Myc-DDK-tagged) - Mouse FERM domain containing 5 (cDNA clone MGC:66559 IMAGE:6416914) |
CNY 4370.00 |
|
| MR206885L4 | Lenti ORF clone of Frmd5 (mGFP-tagged) - Mouse FERM domain containing 5 (cDNA clone MGC:66559 IMAGE:6416914) |
CNY 4370.00 |
