1110039B18Rik (BC022142) Mouse Tagged ORF Clone
CAT＃: MR206845
- TrueORF®
1110039B18Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1110039B18 gene (cDNA clone MGC:37879 IMAGE:5101205)
ORF Plasmid: tGFP
"BC022142" in other vectors (3)
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CNY 3656.00
Product images
CNY 6840.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110039B18Rik; 4921530J21Rik; AI841294; AU018742; C76280 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206845 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGGCCCTGGGGCAAGCGGGTTTCACCAACCTCACTGAGGGACTGAAAGTGTGGCTGGGGATCATGC TGCCTGTGCTGGGCATCAAGGCTCTGTCTCCCTTTGCCATCGCATACCTGGACCGGCTGCTCCTGATGCA TCCCAACCTCACCAAAGGCTTTGGCATGATTGGCCCCAAGGACTTCTTCCCCCTTCTGGACTTTGCCTAT ATGCCCAACAATTCCCTGAGCCCCAGCCTGCAGGAGCAGCTGTGCCAGCTCTTCCCCCGATTGAAGGTGC TGGCATTTGGGGCCAAGCCGGAATCTTCCCTGCACACCTACTTCCCGTCATTCCTGTCCAGAGCCACCCC TAGCTGTCCTGCTGCCATGAAGAAAGAGCTCCTGGCCAGCCTGACCCAGTGCCTGACTGTGGACCCCCTT AGCACTAGTGTCTGGAGACAGCTGTACCCCAAGCATCTGTCACAGTCCAGCCTGCTGCTGGAGCACCTAC TCAAGTCCTGGGAGCACATCCCCAAGAAGGCACGGAAATCTTTACAAGAAACCATCCAGTCCTTGAAGGT TACCAACCAGGAGCTTCTGAAGAAGGGCAGTGGTGGCAGTGAGCATGTTCTCACCTGTGACACAGCCTGC AAGGGCTTGTTGCAACGGGCACGGGGTCCTCGGCCACCCTGGGCCCGGCTATTCCTGCTGCTTCTGGTCT TTGCAGTAGGATTCCTATGTCATGACTTGCGGTCAAACAGCTCTTTGCAGGCCTCCCTCACTGGCCGGCT GCTTCGATCCTCCGGTCTCTTGCCTGTTGGCCAGCAAGTGTGTGCGAGACTCTCCTCCTACAGCCTGCAG AGCTACAACTGGCTGCAGGAGACCTTGCCAGCCTGTGGCTCCCACCTGCTTGCTGTGGTACAGCCCAGTC TGCAGCTGGCCTGGACACACATCTATGCCATATTCAGCTTCCTTTCTGCCCACTGTGCCTCCTACCTTGC CTGCTTCAGTGATAGCCTGGCTGGCTTCTTTCAGAGGGTCCAGCTCCCTGAGGCCCTGCAGCAGCTCTTC CACGCCTTGAAGGAACTGCTGCTGCTCTTCTGCCATAGCGTGCTGCTGCCCACATGGCACTTGCTGCTTG CAGCCCTGGCCCAAGTCCAGGAGCATTGCCATGAGGCCTGCAGAGGGGACGTGACCTGGGACTGCATTAA GACACAGTTGAGCAGAGCTGCCCAATGGACCTGGCTCTGCCTGCAGGATGTCACAGTGGCTTTCTTGGAC TGGGCACTCACCATGATATCCCAGCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206845 protein sequence
Red=Cloning site Green=Tags(s) MWALGQAGFTNLTEGLKVWLGIMLPVLGIKALSPFAIAYLDRLLLMHPNLTKGFGMIGPKDFFPLLDFAY MPNNSLSPSLQEQLCQLFPRLKVLAFGAKPESSLHTYFPSFLSRATPSCPAAMKKELLASLTQCLTVDPL STSVWRQLYPKHLSQSSLLLEHLLKSWEHIPKKARKSLQETIQSLKVTNQELLKKGSGGSEHVLTCDTAC KGLLQRARGPRPPWARLFLLLLVFAVGFLCHDLRSNSSLQASLTGRLLRSSGLLPVGQQVCARLSSYSLQ SYNWLQETLPACGSHLLAVVQPSLQLAWTHIYAIFSFLSAHCASYLACFSDSLAGFFQRVQLPEALQQLF HALKELLLLFCHSVLLPTWHLLLAALAQVQEHCHEACRGDVTWDCIKTQLSRAAQWTWLCLQDVTVAFLD WALTMISQQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC022142 |
| ORF Size | 1287 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC022142, AAH22142 |
| RefSeq Size | 2067 bp |
| RefSeq ORF | 1289 bp |
| Locus ID | 68796 |
| MW | 47.9 kDa |
| Gene Summary | Critical mediator, in cooperation with CASP4, of endoplasmic reticulum-stress induced apoptosis. Required or the activation of CASP4 following endoplasmic reticulum stress (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG206845 | 1110039B18Rik (tGFP-tagged) - Mouse RIKEN cDNA 1110039B18 gene (cDNA clone MGC:37879 IMAGE:5101205) |
CNY 2760.00 |
|
| MR206845L3 | Lenti ORF clone of 1110039B18Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1110039B18 gene (cDNA clone MGC:37879 IMAGE:5101205) |
CNY 4370.00 |
|
| MR206845L4 | Lenti ORF clone of 1110039B18Rik (mGFP-tagged) - Mouse RIKEN cDNA 1110039B18 gene (cDNA clone MGC:37879 IMAGE:5101205) |
CNY 4370.00 |
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