Mto1 (BC063256) Mouse Tagged ORF Clone
CAT＃: MR206766
- TrueORF®
Mto1 (Myc-DDK-tagged) - Mouse mitochondrial translation optimization 1 homolog (S
ORF Plasmid: tGFP
"BC063256" in other vectors (3)
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CNY 4280.00
Product images
CNY 300.00
CNY 6840.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2310039H01Rik; 5730419A02Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206766 representing BC063256
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCTTCTCCGAGGCCGTGGCCATTGGGCCGCAGCGTCCCTCGGCAGACGGCTGCCTTTGCGTCGCC TCCGCAGCGACAGCGCGGCGCCCTGCACCCCGCACTTCGACGTGGTAGTTATCGGAGGAGGGCACGCGGG GACCGAGGCGGCCGCCGCCGCCGCTCGCTGTGGCTCGCGGACCCTGCTTCTCACGCACCGCGTGGACACG ATCGGTCAGATGTCCTGTAATCCTTCCTTTGGTGGCATCGGAAAGGGGCATTTGATGAGAGAAGTGGATG CCTTGGATGGTCTGTGTTCCCGGATCTGTGACCAGTCTGGTATACATTACAAAGTGCTAAACCGGCGTAA GGGCCCTGCTGTGTGGGGACTGAGAGCACAGATTGACCGGAAACTTTATAAACAGAACATGCAGAAAGAA ATCCTGAGCACGCCCCTACTCACTGTTCAGAAGGGAGCTGTAGAAGATCTAGTTCTTGCAGAACCAGAGC CTGGGTACCCTGGGAAGTCCAGGGTCAGAGGTGTTGTCCTGGCAGATGGAAGTACAATTTATGCAGAGAG TGTAATTCTGACTACTGGGACATTTCTGAGAGGCATGATCATAATTGGATTGGAGATGCATCCTGCAGGA CGTTTGGGGGATCAGCCCTCCATAGGGTTGGCTCAGACTCTGGAGAAGTTGGGATTTATGGTAGGAAGAC TAAAGACTGGAACTCCACCCCGACTCGCTAAAGAGTCCATTAATTTCTCCATTCTAAACAAGCACACACC AGATGATCCATCCATACCATTCAGCTTTCTCAGTGACAGCGTGTGGATCAAGCCGGAAGACCAACTGCCA TGTTACTTGACTCACACCAACCCCCGAGTGGATGCGATTGTCCTTGAGAACCTTCACCTGAATAGCCACG TTCAGGAAACTACAAAAGGGCCCCGATACTGTCCCTCTATTGAATCAAAGGTTTTACGTTTTCCAAATCG TCTGCATCAGGTTTGGTTGGAGCCTGAAGGAATGGATTCCGACCTCATCTACCCGCAAGGGTTATCTGTT ACACTGCCAGCTGAGTTACAGGAGAAAATGATAACATGCATCAGAGGCTTGGAGAAAGCCAAAATGGTTC ACCCAGGCTATGGTGTGCAGTATGATTATTTAGACCCCCGTCAGATCAGTCCTTCCCTCGAGACACACTT GGTTCAGCGGCTGTTCTTTGCTGGACAGATAAATGGAACCACTGGCTATGAGGAAGCTGCAGCTCAGTCC TCTTCTAGGGGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | BC063256 |
| ORF Size | 1272 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC063256 |
| RefSeq Size | 5221 bp |
| RefSeq ORF | 1274 bp |
| Locus ID | 68291 |
| MW | 191.4 kDa |
| Gene Summary | Involved in the 5-carboxymethylaminomethyl modification (mnm(5)s(2)U34) of the wobble uridine base in mitochondrial tRNAs.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218398 | Mto1 (untagged) - Mouse mitochondrial translation optimization 1 homolog (S, (10ug) |
CNY 6940.00 |
|
| MG206766 | Mto1 (tGFP-tagged) - Mouse mitochondrial translation optimization 1 homolog (S |
CNY 4750.00 |
|
| MR206766L3 | Lenti ORF clone of Mto1 (Myc-DDK-tagged) - Mouse mitochondrial translation optimization 1 homolog (S |
CNY 6180.00 |
