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Syt5 (NM_016908) Mouse Tagged ORF Clone
CAT＃: MR206024
- TrueORF®
Syt5 (Myc-DDK-tagged) - Mouse synaptotagmin V (Syt5)
ORF Plasmid: tGFP
"NM_016908" in other vectors (4)
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CNY 3,330.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SytV |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206024 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCCGGAACCCCCGACCCTGGGGTCTCCAGCGCCTAAGACACCTCCAGACTCCAGCCGCATCAGGC AGGGCGCAGTGCCTGCCTGGGTCCTGGCTACCATCGTGCTGGGCTCGGGCCTCCTGGTCTTCAGCAGTTG TTTCTGTCTCTACCGGAAGCGCTGTCGGAGACGGATGGGCAAAAAGAGTCAGGCCCAAGCCCAAGTCCAT CTTCAGGAAGTGAAGGAGCTGGGCCGGAGTTACATAGATAAGGTTCAGCCTGAAATAGAGGAGCTGGACC GGTCACCATCCATGCCTGGACAGCAGGTATCAGACAAACACCAGCTAGGCCGACTGCAGTATTCACTGGA CTATGACTTCCAGACTGGCCAGCTCCTGGTGGGCATCTTGCAAGCTCAGGGACTGGCTGCGTTGGACCTA GGAGGTTCCTCAGATCCCTATGTTAGTGTCTATCTGCTGCCAGACAAGCGGAGGCGACATGAGACCAAGG TGCATCGGCAGACCCTGAATCCACACTTTGGAGAGACATTTGCCTTCAAGGTCCCATACGTGGAACTAGG GGGCAGAGTGCTGGTCATGGCGGTGTATGACTTTGATCGGTTCTCCCGCAACGATGCCATCGGGGAGGTG CGGGTGCCTATGAGTTCAGTGAACCTGGGGCGGCCGGTGCAGGCCTGGAGAGAGCTGCAGGTGGCTCCCA AAGAGGAGCAAGAGAAACTGGGGGACATCTGCTTCTCTCTCCGTTATGTCCCCACGGCCGGGAAGCTCAC CGTCATTGTCTTGGAAGCTAAAAACCTGAAGAAAATGGATGTAGGAGGACTCTCAGATCCCTATGTGAAG GTGCACCTGCTCCAGGGAGGTAAAAAGGTTCGCAAGAAGAAAACCACCATTAAGAAGAACACCCTGAACC CCTATTACAACGAGGCCTTCAGCTTTGAGGTGCCCTGTGACCAGGTGCAGAAAGTCCAGGTGGAGCTGAC TGTTCTGGATTATGACAAACTGGGGAAGAATGAGGCCATCGGGAGAGTGGCGGTGGGCGCAGCAGTCGGT GGGGCTGGCCTGCGGCACTGGGCTGACATGTTGGCCAACCCCAGGCGCCCCATTGCCCAGTGGCACTCTC TGCGGCCCCCTGACAGAGCCAGGCCAATACCAGCACCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206024 protein sequence
Red=Cloning site Green=Tags(s) MFPEPPTLGSPAPKTPPDSSRIRQGAVPAWVLATIVLGSGLLVFSSCFCLYRKRCRRRMGKKSQAQAQVH LQEVKELGRSYIDKVQPEIEELDRSPSMPGQQVSDKHQLGRLQYSLDYDFQTGQLLVGILQAQGLAALDL GGSSDPYVSVYLLPDKRRRHETKVHRQTLNPHFGETFAFKVPYVELGGRVLVMAVYDFDRFSRNDAIGEV RVPMSSVNLGRPVQAWRELQVAPKEEQEKLGDICFSLRYVPTAGKLTVIVLEAKNLKKMDVGGLSDPYVK VHLLQGGKKVRKKKTTIKKNTLNPYYNEAFSFEVPCDQVQKVQVELTVLDYDKLGKNEAIGRVAVGAAVG GAGLRHWADMLANPRRPIAQWHSLRPPDRARPIPAP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016908 |
| ORF Size | 1161 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016908.2, NP_058604.1 |
| RefSeq Size | 1764 bp |
| RefSeq ORF | 1161 bp |
| Locus ID | 53420 |
| UniProt ID | Q9R0N5 |
| MW | 43.1 kDa |
| Gene Summary | May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Regulates the Ca(2+)-dependent secretion of norepinephrine in PC12 cells. Required for export from the endocytic recycling compartment to the cell surface.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203087 | Syt5 (untagged) - Mouse synaptotagmin V (Syt5), (10ug) |
CNY 2,000.00 |
|
| MG206024 | Syt5 (tGFP-tagged) - Mouse synaptotagmin V (Syt5) |
CNY 3,710.00 |
|
| MR206024L3 | Lenti ORF clone of Syt5 (Myc-DDK-tagged) - Mouse synaptotagmin V (Syt5) |
CNY 5,230.00 |
|
| MR206024L4 | Lenti ORF clone of Syt5 (mGFP-tagged) - Mouse synaptotagmin V (Syt5) |
CNY 5,230.00 |
