Acot5 (BC016076) Mouse Tagged ORF Clone
CAT＃: MR204894
- TrueORF®
Acot5 (Myc-DDK-tagged) - Mouse acyl-CoA thioesterase 5 (cDNA clone MGC:27572 IMAGE:4485973)
ORF Plasmid: tGFP
"BC016076" in other vectors (4)
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CNY 2400.00
CNY 6365.00
Product images
CNY 300.00
CNY 6840.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BC016076; PTE-Ic |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR204894 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCCAACAGTAAGCCTGGAGCCCACGGGCCACAGCTGCTGGGACGAACCGCTGAGCATCGCTGTGC GCGGCTTGGCCCCGGAGCAGCCCGTCACACTGCGCACTGCCCTGCGCGACGAGAAGGGCGCGCTCTTCCG AGCCCACGCGCGCTACCGCGCGGACTCCCACGGCGAGCTGGACCTGGCGCGCACGCCCGCGCTGGGCGGC AGCTTCTCGGGGCTCGAGCCCATGGGGCTGCTCTGGGCCATGGAGCCCGATCGGCCTTTCTGGCGCCTGA TCAAGCGCGACGTGCAGACGCCCTTCGTGGTGGAGCTGGAGGTGCTGGACGGACACGAGCCCGACGGCGG GCGGCTGCTGGCGCGGGCGGTGCACGAGCGTCACTTCATGGCTCCCGGGGTGCGGCGCGTGCCCGTGCGC GAGGGCCGGGTGCGCGCCACGCTCTTTCTGCCCCCAGGAACTGGACCCTTTCCTGGGATCATAGACCTTT TTGGAGTTGGAGGTGGCCTTCTGGAGTATCGGGCGAGCCTGCTGGCTGGGAAGGGCTTTGCCGTCATGGC TCTGGCTTATTATAAATATGATGACCTCCCTAAGGTTATAGACATCCTTCATCTGGAGTACTTTGAGGAA GCTGTGACCTACCTGCTCAGTCACCCTCAGGTCAAGGGTCCAGGAGTTGGCCTGCTAGGGATTTCCAAAG GGGCTGAACTCTCCCTCTCCATGGCTTCTTTCCTGAAAGGCATCACGGCCGCTGTCGTAATCAATGGCGC CACGGTCAACGTTATTTCAACATTATACTACAAGGAAGAGAGTCTGCCTGGTTTGGGCATGCACCTAGAA AGGATCAAGGTCAGGACGACCACAACTGGAAGAGCGAGTTCTATGCCAGAGAGGCCTCCAAACGCCTGCA GGCGCACGGGAAGGAGAAGCCCCAGATTGTCTGCTACCCCAAAACAGGACACCACATTGAACCCCCTTAC ATCCCTTGGTCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204894 protein sequence
Red=Cloning site Green=Tags(s) MVPTVSLEPTGHSCWDEPLSIAVRGLAPEQPVTLRTALRDEKGALFRAHARYRADSHGELDLARTPALGG SFSGLEPMGLLWAMEPDRPFWRLIKRDVQTPFVVELEVLDGHEPDGGRLLARAVHERHFMAPGVRRVPVR EGRVRATLFLPPGTGPFPGIIDLFGVGGGLLEYRASLLAGKGFAVMALAYYKYDDLPKVIDILHLEYFEE AVTYLLSHPQVKGPGVGLLGISKGAELSLSMASFLKGITAAVVINGATVNVISTLYYKEESLPGLGMHLE RIKVRTTTTGRASSMPERPPNACRRTGRRSPRLSATPKQDTTLNPLTSLGP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | BC016076 |
| ORF Size | 993 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC016076, AAH16076 |
| RefSeq Size | 1416 bp |
| RefSeq ORF | 995 bp |
| Locus ID | 217698 |
| MW | 36 kDa |
| Gene Summary | Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:16940157). Mainly active on medium-chain acyl-CoAs (PubMed:15007068). Seems to be involved in intraperoxisomal regulation of acyl-CoA levels, but not CoASH levels (PubMed:15007068). May have a function in termination of beta-oxidation of fatty acids (PubMed:15007068).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217985 | Acot5 (untagged) - Mouse acyl-CoA thioesterase 5 (cDNA clone MGC:27572 IMAGE:4485973), (10ug) |
CNY 6940.00 |
|
| MG204894 | Acot5 (tGFP-tagged) - Mouse acyl-CoA thioesterase 5 (cDNA clone MGC:27572 IMAGE:4485973) |
CNY 2850.00 |
|
| MR204894L3 | Lenti ORF clone of Acot5 (Myc-DDK-tagged) - Mouse acyl-CoA thioesterase 5 (cDNA clone MGC:27572 IMAGE:4485973) |
CNY 4750.00 |
|
| MR204894L4 | Lenti ORF clone of Acot5 (mGFP-tagged) - Mouse acyl-CoA thioesterase 5 (cDNA clone MGC:27572 IMAGE:4485973) |
CNY 4750.00 |
