D10Ertd610e (BC054833) Mouse Tagged ORF Clone
CAT＃: MR204889
- TrueORF®
D10Ertd610e (Myc-DDK-tagged) - Mouse DNA segment, Chr 10, ERATO Doi 610, expressed (cDNA clone MGC:62781 IMAGE:6439728)
ORF Plasmid: tGFP
"BC054833" in other vectors (4)
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CNY 2400.00
CNY 6365.00
Product images
CNY 6840.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2410008H17Rik; D10Ertd610e; GEFT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR204889 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATGTGGTGTACTGCCAGAATAAACCCAAGTCAGAGCATGTGCTGTCAGAGTTTGGGGACAGCTACT TTGAGGAGCTGAGGCAGCAGCTGGGACACCGACTGCAGCTCAATGACCTCCTCATTAAACCAGTGCAGCG AATCATGAAATACCAGTTGCTGCTGAAGGATTTTCTAAAGTATTACAGAAGAGCTGGGAAGGATACTGAA GAGCTGGAGCAAGCTGTGGAGGTCATGTGTTTCGTACCTAAGCGATGCAATGATATGATGTCCCTGGGGA GACTTCGGGGATTTGAGGGAAAACTGACTGCTCAGGGGAAGCTCTTGGGCCAGGACACATTCTTGGTTAC AGAGCCTGAAGCTGGGGGTCTGCTTTCTTCCCGGGGTCGGGAGAGGCGTGTCTTCCTGTTCGAGCAAATC ATCATCTTCAGTGAGGCCCTGGGAGGAGGAGGCCGAGGTGGCGCGCAGCCTGGCTACGTGTACAAGAACA GCATCAAGGTGAGCTGCCTAGGGCTGGAGGGGAATCTCCAAGGCAACCCTTGCCGGTTTGCGCTGACCTC TAGAGGGCCAGAAGGTGGGATCCAGCGCTACGTTCTGCAGGCTTCAGACCCTGCTGTCAGTCAAGCCTGG ATAAAGCAAGTGGCCCAGATCCTGGAAAGCCAGCGTGACTTTCTCAACGGTGAAGCTCCCATCCTCTCCC TTCAGAGGCTCCCAAAGCTATCTGACCTCCTGAACCCCTTCCCCAGCCACGCAGTCTCCAACCCTTGCTT CTTTGAGCCTAACTTCTCTCCATCCTACACCCACCCGAAACCCTCTAAAGTTCCAAGCACCTTACATTGG CCAAATGAGGAAGAAGGTGAGGCAGGGAAAGTGATCGGTCTCTTCTGTAATACATCTCTGTTCCTTCCCT TGGCCCAGCATTACAGTCACCCATTGAATACCAGAGACGTGAGAGCCAGACCAATAGCCTGGGGCGACCA GGAGGGCCTTGGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204889 protein sequence
Red=Cloning site Green=Tags(s) MYVVYCQNKPKSEHVLSEFGDSYFEELRQQLGHRLQLNDLLIKPVQRIMKYQLLLKDFLKYYRRAGKDTE ELEQAVEVMCFVPKRCNDMMSLGRLRGFEGKLTAQGKLLGQDTFLVTEPEAGGLLSSRGRERRVFLFEQI IIFSEALGGGGRGGAQPGYVYKNSIKVSCLGLEGNLQGNPCRFALTSRGPEGGIQRYVLQASDPAVSQAW IKQVAQILESQRDFLNGEAPILSLQRLPKLSDLLNPFPSHAVSNPCFFEPNFSPSYTHPKPSKVPSTLHW PNEEEGEAGKVIGLFCNTSLFLPLAQHYSHPLNTRDVRARPIAWGDQEGLG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | BC054833 |
| ORF Size | 993 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC054833, AAH54833 |
| RefSeq Size | 1732 bp |
| RefSeq ORF | 995 bp |
| Locus ID | 52666 |
| MW | 37.1 kDa |
| Gene Summary | May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. Links specifically G alpha q/11-coupled receptors to RHOA activation (By similarity). May be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. Involved in skeletal myogenesis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218309 | D10Ertd610e (untagged) - Mouse DNA segment, Chr 10, ERATO Doi 610, expressed (cDNA clone MGC:62781 IMAGE:6439728), (10ug) |
CNY 6940.00 |
|
| MG204889 | D10Ertd610e (tGFP-tagged) - Mouse DNA segment, Chr 10, ERATO Doi 610, expressed (cDNA clone MGC:62781 IMAGE:6439728) |
CNY 2850.00 |
|
| MR204889L3 | Lenti ORF clone of D10Ertd610e (Myc-DDK-tagged) - Mouse DNA segment, Chr 10, ERATO Doi 610, expressed (cDNA clone MGC:62781 IMAGE:6439728) |
CNY 4750.00 |
|
| MR204889L4 | Lenti ORF clone of D10Ertd610e (mGFP-tagged) - Mouse DNA segment, Chr 10, ERATO Doi 610, expressed (cDNA clone MGC:62781 IMAGE:6439728) |
CNY 4750.00 |
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