Jtv1 (BC026958) Mouse Tagged ORF Clone
CAT＃: MR204623
- TrueORF®
Jtv1 (Myc-DDK-tagged) - Mouse JTV1 gene (cDNA clone MGC:30261 IMAGE:4013019)
ORF Plasmid: tGFP
"BC026958" in other vectors (3)
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CNY 3230.00
Product images
CNY 300.00
CNY 6840.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC37389, MGC30261, MGC36346, MGC36522 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR204623 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGATGTACCAGGTAAAGCCCTATCATGGAGGCAGCGCACCTCTGCGTGTAGAGCTTCCAACCTGCA TGTACCGGCTCCCCAACGTGCACAGCAAGACCACCAACCCCGCCACCGACGCGGGCCACGTGCAGGAAAC ATCCGAGCCTTCTTTGCAAGCCCTTGAATCTCGCCAAGATGATATTTTAAAACGCTTGTATGAGTTGAAG GCAGCAGTCGATGGCCTTTCAAAGATGATTCACACCCCAGATGCAGACTTGGACGTAACCAACATCCTGC AAGCTGATGAGCCCACAACTTTAGCCACAAACACATTGGACTTGAATTCCGTGCTTGGAAAGGACTATGG GGCGCTGAAAGACATTGTGATCAACGCAAACCCAGCCTCCCCACCACTGTCCCTGCTTGTGCTGCATAGG CTGCTCTGTGAACGCTACAGGGTCCTGTCCACTGTGCACACACATTCGTCTGTCAAGAATGTGCCTGAGA ATCTTGTCAAGTGCTTCGGGGAGCAGGCTAGGAAGCAGTCCCGCCACGAGTATCAGCTGGGCTTCACTCT GATTTGGAAGAATGTGCCCAAGACACAGATGAAGTTCAGCGTACAAACCATGTGCCCCATTGAAGGAGAA GGGAACATCGCACGTTTCCTGTTCTCTCTGTTTGGCCAGAAGCATAATGCTGTCACCCTCACCCTCATCG ATAGCTGGGTGGATATCGCCATGTTTCAGCTTCGAGAAGGCAGCAGTAAAGAAAAAGCGGCCGTGTTCCG CTCTATGAACTCCGCTTTGGGGAGGAGCCCGTGGCTGGTTGGGAATGAGCTCACTGTGGCAGATGTGGTG CTGTGGTCTGTGCTCCAGCAGACTGGGGGCAGCAGTGGGGCAGCACCCACCAATGTGCAGCGGTGGCTTA AGTCCTGTGAGAACCTGGCCCCCTTCAGCACTGCCCTTCAGCTCCTTAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204623 protein sequence
Red=Cloning site Green=Tags(s) MPMYQVKPYHGGSAPLRVELPTCMYRLPNVHSKTTNPATDAGHVQETSEPSLQALESRQDDILKRLYELK AAVDGLSKMIHTPDADLDVTNILQADEPTTLATNTLDLNSVLGKDYGALKDIVINANPASPPLSLLVLHR LLCERYRVLSTVHTHSSVKNVPENLVKCFGEQARKQSRHEYQLGFTLIWKNVPKTQMKFSVQTMCPIEGE GNIARFLFSLFGQKHNAVTLTLIDSWVDIAMFQLREGSSKEKAAVFRSMNSALGRSPWLVGNELTVADVV LWSVLQQTGGSSGAAPTNVQRWLKSCENLAPFSTALQLLK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | BC026958 |
| ORF Size | 960 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC026958, AAH26958 |
| RefSeq Size | 1162 bp |
| RefSeq ORF | 962 bp |
| Locus ID | 231872 |
| MW | 35.4 kDa |
| Gene Summary | Required for assembly and stability of the aminoacyl-tRNA synthase complex (PubMed:12060739). Mediates ubiquitination and degradation of FUBP1, a transcriptional activator of MYC, leading to MYC down-regulation which is required for aveolar type II cell differentiation (PubMed:12819782). Blocks MDM2-mediated ubiquitination and degradation of p53/TP53 (PubMed:18695251). Functions as a proapoptotic factor (PubMed:16135753).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG204623 | Jtv1 (tGFP-tagged) - Mouse JTV1 gene (cDNA clone MGC:30261 IMAGE:4013019) |
CNY 3520.00 |
|
| MR204623L3 | Lenti ORF clone of Jtv1 (Myc-DDK-tagged) - Mouse JTV1 gene (cDNA clone MGC:30261 IMAGE:4013019) |
CNY 5130.00 |
|
| MR204623L4 | Lenti ORF clone of Jtv1 (mGFP-tagged) - Mouse JTV1 gene (cDNA clone MGC:30261 IMAGE:4013019) |
CNY 5130.00 |
