9530077C05Rik (BC039998) Mouse Tagged ORF Clone

CAT＃: MR203696

TrueORF^®

9530077C05Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 9530077C05 gene (cDNA clone MGC:48244 IMAGE:3326047)

ORF Plasmid: DDK tGFP

Datasheet

SDS

"BC039998" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 2760.00

货期*

4周

规格

Product images

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Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1110003N12Rik; Kiaa0895; mKIAA0895
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR203696 representing BC039998 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGACTTTACAAAAATATGGAAGTTATGAAAAATTTGAACAAGCTACTGGTGGTAGCTTGTTGT CTAAAACTCGAATCTGGAGTCATGTTAGAAAGTACATGGTGAAGGAAGGCTGCCTGGGAGAGATTGTAGT TCATCTCACCGAAGACCTGCTTTCCAGGGCATCCATGACGGTAGTAAACGGATGCCCGACTCTAACCATC AATATTTCTACTGCACGAGAGCACTGGCTGGAGGGCATGCTGAGGCACGAGATAGGCACACATTATTTTC GGGGTATTAACAACCTTCAGCAACCGTGGAACAGTTGGATTGGCCGAAAAAAACATGAGCTAAAGCCCAA CAACCCAACGGAGGAAGGGCTGGCAAGCATCCACAGTGTCCTGTTCAGAAAAGACCCCTTCCTGTGGAGG GCCGCTCTCCTGTACTACACGGTGTATAAAGCCAGCCACATGTCCTTCTGTGAACTCTTCAAAGACATTG GCAAGTTTGTCAAGGACCCTAATACAAGATGGGACTATTGTGTAAGAGCCAAGAGAGGGTGGACTGACAC TTCTGAGCCCGGGTGTTTCAGTAAGGACCAGGTCTACTTGGATGGAGTCCTTCAAATCCTGCGATTCAGA GAGTCCATAGACTTCCATCTGCTGACTGCCCTGGGCAAGGTATCTTACGAAGATGTGGATCGCTTAAAAG AATTGGCAGTTACTGAAAACATGCGAGTCCCACATTTCCTTCATGATCACAGCCGGTACATGGAGCACTT AGAGAGGATCATGGAGGTGAACGAGCTGACAGACGCTGAGCTGAAAAACCTCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR203696 representing BC039998 Red=Cloning site Green=Tags(s) MELTLQKYGSYEKFEQATGGSLLSKTRIWSHVRKYMVKEGCLGEIVVHLTEDLLSRASMTVVNGCPTLTI NISTAREHWLEGMLRHEIGTHYFRGINNLQQPWNSWIGRKKHELKPNNPTEEGLASIHSVLFRKDPFLWR AALLYYTVYKASHMSFCELFKDIGKFVKDPNTRWDYCVRAKRGWTDTSEPGCFSKDQVYLDGVLQILRFR ESIDFHLLTALGKVSYEDVDRLKELAVTENMRVPHFLHDHSRYMEHLERIMEVNELTDAELKNLI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI Cloning Scheme for this gene
ACCN	BC039998
ORF Size	825 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC039998.1
RefSeq Size	1501 bp
RefSeq ORF	827 bp
Locus ID	68283
MW	32.1 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
MG203696	9530077C05Rik (tGFP-tagged) - Mouse RIKEN cDNA 9530077C05 gene (cDNA clone MGC:48244 IMAGE:3326047)	10 ug 产品选项验证失败所有选项都是必需的	CNY 3040.00
MR203696L3	Lenti ORF clone of 9530077C05Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 9530077C05 gene (cDNA clone MGC:48244 IMAGE:3326047)	10 ug 产品选项验证失败所有选项都是必需的	CNY 4660.00
MR203696L4	Lenti ORF clone of 9530077C05Rik (mGFP-tagged) - Mouse RIKEN cDNA 9530077C05 gene (cDNA clone MGC:48244 IMAGE:3326047)	10 ug 产品选项验证失败所有选项都是必需的	CNY 4660.00

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