Tef (BC017689) Mouse Tagged ORF Clone
CAT＃: MR203666
- TrueORF®
Tef (Myc-DDK-tagged) - Mouse thyrotroph embryonic factor (cDNA clone MGC:19233 IMAGE:4242534)
ORF Plasmid: tGFP
"BC017689" in other vectors (3)
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CNY 2400.00
CNY 6365.00
Product images
CNY 6840.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2310028D20Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR203666 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACATGCCTGAGGTCCTCAAGTCCCTGCTGGAGCATTCTTTGCCTTGGTCAGAGAAGAAGGCAGATA AGGAAAAGGGGAAGGAAAAGCTGGAGGAAGACGAGTCAGCAGCAGCCAGTACCATGGCCGTCTCTGCCTC CCTCATGCCACCCATCTGGGACAAGACCATCCCCTACGATGGCGAGTCCTTCCACCTCGAGTACATGGAC CTGGATGAGTTCCTGCTGGAGAACGGCATCCCTGCCAGCCCCACCCACCTGGCACAGAACCTGCTGCTGC CTGTGGCAGAGCTTGAAGGAAAGGAGTCTGCCAGCTCTTCCACGGCATCGCCACCATCTTCCTCTACTGC CATCTTTCAGCCCTCGGAAACCGTGTCCAGCACAGAATCGTCCTTGGAAAAGGAGAGGGAGACCCCAAGT CCCATCGACCCCAGCTGTGTGGAGGTCGATGTGAACTTCAACCCTGACCCTGCCGACCTGGTCCTCTCTA GTGTACCAGGGGGGGAGCTCTTCAACCCTCGGAAGCACAGGTTTGCAGAGGAGGACCTGAAGCCCCAGCC CATGATCAAAAAAGCCAAGAAAGTCTTTGTCCCTGATGAACAGAAGGATGAAAAGTATTGGACAAGACGT AAGAAGAACAATGTGGCAGCTAAACGCTCCCGGGATGCCAGGCGCCTGAAGGAGAACCAGATCACCATCC GGGCAGCCTTCCTGGAGAAGGAGAACACAGCCCTGCGGACGGAGGTTGCCGAGCTTCGCAAGGAGGTGGG CAAGTGCAAGACCATCGTGTCCAAGTACGAGACCAAGTACGGGCCCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203666 protein sequence
Red=Cloning site Green=Tags(s) MDMPEVLKSLLEHSLPWSEKKADKEKGKEKLEEDESAAASTMAVSASLMPPIWDKTIPYDGESFHLEYMD LDEFLLENGIPASPTHLAQNLLLPVAELEGKESASSSTASPPSSSTAIFQPSETVSSTESSLEKERETPS PIDPSCVEVDVNFNPDPADLVLSSVPGGELFNPRKHRFAEEDLKPQPMIKKAKKVFVPDEQKDEKYWTRR KKNNVAAKRSRDARRLKENQITIRAAFLEKENTALRTEVAELRKEVGKCKTIVSKYETKYGPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC017689 |
| ORF Size | 819 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC017689, AAH17689 |
| RefSeq Size | 4018 bp |
| RefSeq ORF | 821 bp |
| Locus ID | 21685 |
| MW | 30.6 kDa |
| Gene Summary | Transcription factor that binds to and transactivates the TSHB promoter. Binds to a minimal DNA-binding sequence 5'-[TC][AG][AG]TTA[TC][AG]-3' (By similarity). Also activates the telokin promoter in smooth muscle-specific and calcium-dependent manner.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG203666 | Tef (tGFP-tagged) - Mouse thyrotroph embryonic factor (cDNA clone MGC:19233 IMAGE:4242534) |
CNY 4000.00 |
|
| MR203666L3 | Lenti ORF clone of Tef (Myc-DDK-tagged) - Mouse thyrotroph embryonic factor (cDNA clone MGC:19233 IMAGE:4242534) |
CNY 4750.00 |
|
| MR203666L4 | Lenti ORF clone of Tef (mGFP-tagged) - Mouse thyrotroph embryonic factor (cDNA clone MGC:19233 IMAGE:4242534) |
CNY 4750.00 |
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