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H2-Ea-ps (NM_010381) Mouse Tagged ORF Clone
CAT＃: MR203273
- TrueORF®
H2 (Myc-DDK-tagged) - Mouse histocompatibility 2, class II antigen E alpha, pseudogene (H2-Ea-ps)
ORF Plasmid: tGFP
"NM_010381" in other vectors (4)
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CNY 1,416.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI323765; E-alpha-f; H-2Ea; H2-Ea; I-Ealpha; Ia-3; Ia3; MHC-H2-Ea |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR203273 representing NM_010381
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACAATTGGAGCCCTGCTGTTAAGATTTTTCTTCATTGCTGTTCTGATGAGCTCCCAGAAGTCAT GGGCTATCAAAGAGGAACACACCATCATCCAGGCGGAGTTCTATCTTTTACCAGACAAACGTGGAGAGTT TATGTTTGACTTTGACGGCGATGAGATTTTCCATGTAGACATTGAAAAGTCAGAGACCATCTGGAGACTT GAAGAATTTGCAAAGTTTGCCAGCTTTGAGGCTCAGGGTGCACTGGCTAATATAGCTGTGGACAAAGCTA ACCTGGATGTCATGAAAGAGCGTTCCAACAACACTCCAGATGCCAACGTGGCCCCAGAGGTGACTGTACT CTCCAGAAGCCCTGTGAACCTGGGAGAGCCCAACATCCTCATCTGTTTCATTGACAAGTTCTCCCCTCCA GTGGTCAATGTCACCTGGTTCCGGAATGGACGGCCTGTCACCGAAGGCGTGTCAGAGACAGTGTTTCTCC CGAGGGACGATCACCTCTTCCGCAAATTCCACTATCTGACCTTCCTGCCCTCCACAGATGATTTCTATGA CTGTGAGGTGGATCACTGGGGTTTGGAGGAGCCTCTGCGGAAGCACTGGGAGTTTGAAGAGAAAACCCTC CTCCCAGAAACTACAGAGAATGTCGTGTGTGCTCTTGGGTTGTTTGTGGGTCTGGTGGGCATCGTTGTGG GGATTATCCTCATCATGAAGGGTATTAAAAAACGCAATGTTGTAGAACGCCGACAAGGAGCCCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203273 representing NM_010381
Red=Cloning site Green=Tags(s) MATIGALLLRFFFIAVLMSSQKSWAIKEEHTIIQAEFYLLPDKRGEFMFDFDGDEIFHVDIEKSETIWRL EEFAKFASFEAQGALANIAVDKANLDVMKERSNNTPDANVAPEVTVLSRSPVNLGEPNILICFIDKFSPP VVNVTWFRNGRPVTEGVSETVFLPRDDHLFRKFHYLTFLPSTDDFYDCEVDHWGLEEPLRKHWEFEEKTL LPETTENVVCALGLFVGLVGIVVGIILIMKGIKKRNVVERRQGAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010381 |
| ORF Size | 765 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010381.2, NP_034511.2 |
| RefSeq Size | 887 bp |
| RefSeq ORF | 768 bp |
| Locus ID | 100504404 |
| UniProt ID | P04224 |
| MW | 29.6 kDa |
| Gene Summary | This locus belongs to the class II major histocompatibility complex (MHC) family of genes, which encode immune response (Ia) antigens that function in the T-cell-dependent immune response. This family member has multiple haplotypes, some of which result in the production of an E-alpha subunit that combines with an E-beta subunit to form a functional E complex at the cell surface. Other haplotypes, including that of the reference genome allele, contain mutations and they thus represent polymorphic pseudogenes that do not produce functional products. These mutations include frameshifting indels, nonsense mutations, and deletions of larger regions. The reference genome haplotype contains a deletion at the 5' end of the gene, including the core promoter region and the transcription start site, and therefore no transcripts result from this haplotype. [provided by RefSeq, Aug 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208624 | H2 (untagged) - Mouse histocompatibility 2, class II antigen E alpha, pseudogene (H2-Ea-ps), (10ug) |
CNY 3,990.00 |
|
| MG203273 | H2 (tGFP-tagged) - Mouse histocompatibility 2, class II antigen E alpha (H2-Ea) |
CNY 2,850.00 |
|
| MR203273L3 | Lenti ORF clone of H2 (Myc-DDK-tagged) - Mouse histocompatibility 2, class II antigen E alpha, pseudogene (H2-Ea-ps) |
CNY 4,750.00 |
|
| MR203273L4 | Lenti ORF clone of H2 (mGFP-tagged) - Mouse histocompatibility 2, class II antigen E alpha, pseudogene (H2-Ea-ps) |
CNY 4,750.00 |
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