Letmd1 (BC021361) Mouse Tagged ORF Clone

CAT#: MR202102

  • TrueORF®

Letmd1 (Myc-DDK-tagged) - Mouse LETM1 domain containing 1 (cDNA clone MGC:29318 IMAGE:5010892)

ORF Plasmid: DDK tGFP



  "BC021361" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2400.00

CNY 2945.00


货期*
现货

规格
    • 10 ug

Product images

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DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms Mccr, MCC-32, HCCR1, HCCR-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR202102 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTACCTGTTTCCTAGGCAACTGCTAGTCAAGCATTTCTGGACCCCCAAACAACAAATTGATTTCTTGG
ACGTTTATCATGGTCTCCGGAGGCGGTCCCACTCGGAAGTCATCACACATCTAAGAAGGGCCAGCACTTT
TGTTTCTCATGAGAAACTCCGGAGGCAGCTGACAGACCTGTGCACCAAGGTGCAGAGTGGCACCCACCCA
GCAGCACAGGACGTCCTGGCTCTCAGAGATTGCTTCTCTACCTACCCCTTGGGCTTCAGCCAGCTCCAAG
CCTCGCAGATGAGAGCCTTGAGCCAGGCCATGCTCCTCACACCTTACCTGCCCCCGCCCTTGCTGAGGCA
GCGGCTAAAGAGTCACACCACCGTGATCCACCAGCTGGACAGGGCTCTGGCAAAGCTGGGGATTGGCCAG
CTGACTGCACAGGAAGTGAAATCGGCTTGCTATCTTCGTGGCTTGAACTCTACCCACATTGCTGACGACA
GGTGTCGAGCCTGGCTGGGGGAATGGTTGCACATCTCCTGTAGCCTGAAAGAACCCGAGCTGTCCCTCTT
GCTGCACAACGTGGTCCTGCTCTCCACCAACTACCTTGAGACAAGGCGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR202102 protein sequence
Red=Cloning site Green=Tags(s)

MYLFPRQLLVKHFWTPKQQIDFLDVYHGLRRRSHSEVITHLRRASTFVSHEKLRRQLTDLCTKVQSGTHP
AAQDVLALRDCFSTYPLGFSQLQASQMRALSQAMLLTPYLPPPLLRQRLKSHTTVIHQLDRALAKLGIGQ
LTAQEVKSACYLRGLNSTHIADDRCRAWLGEWLHISCSLKEPELSLLLHNVVLLSTNYLETRR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN BC021361
ORF Size 609 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC021361, AAH21361
RefSeq Size 2179 bp
RefSeq ORF 611 bp
Locus ID 68614
MW 23.4 kDa
Gene Summary Involved in tumorigenesis and may function as a negative regulator of the p53/TP53.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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