Icos (NM_017480) Mouse Tagged ORF Clone
CAT#: MR202040
- TrueORF®
Icos (Myc-DDK-tagged) - Mouse inducible T-cell co-stimulator (Icos)
ORF Plasmid: tGFP
"NM_017480" in other vectors (4)
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CNY 1,416.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AILIM; CCLP; CRP-1; H4; Ly115 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR202040 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCCGTACTTCTGCCGTGTCTTTGTCTTCTGCTTCCTAATCAGACTTTTAACAGGAGAAATCAATG GCTCGGCCGATCATAGGATGTTTTCATTTCACAATGGAGGTGTACAGATTTCTTGTAAATACCCTGAGAC TGTCCAGCAGTTAAAAATGCGATTGTTCAGAGAGAGAGAAGTCCTCTGCGAACTCACCAAGACCAAGGGA AGCGGAAATGCGGTGTCCATCAAGAATCCAATGCTCTGTCTATATCATCTGTCAAACAACAGCGTCTCTT TTTTCCTAAACAACCCAGACAGCTCCCAGGGAAGCTATTACTTCTGCAGCCTGTCCATTTTTGACCCACC TCCTTTTCAAGAAAGGAACCTTAGTGGAGGATATTTGCATATTTATGAATCCCAGCTCTGCTGCCAGCTG AAGCTCTGGCTACCCGTAGGGTGTGCAGCTTTCGTTGTGGTACTCCTTTTTGGATGCATACTTATCATCT GGTTTTCAAAAAAGAAATACGGATCCAGTGTGCATGACCCTAATAGTGAATACATGTTCATGGCGGCAGT CAACACAAACAAAAAGTCTAGACTTGCAGGTGTGACCTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202040 protein sequence
Red=Cloning site Green=Tags(s) MKPYFCRVFVFCFLIRLLTGEINGSADHRMFSFHNGGVQISCKYPETVQQLKMRLFREREVLCELTKTKG SGNAVSIKNPMLCLYHLSNNSVSFFLNNPDSSQGSYYFCSLSIFDPPPFQERNLSGGYLHIYESQLCCQL KLWLPVGCAAFVVVLLFGCILIIWFSKKKYGSSVHDPNSEYMFMAAVNTNKKSRLAGVTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_017480 |
ORF Size | 603 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_017480.2 |
RefSeq Size | 3272 bp |
RefSeq ORF | 603 bp |
Locus ID | 54167 |
UniProt ID | Q9WVS0 |
MW | 22.7 kDa |
Gene Summary | Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
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ICOS agonism by JTX-2011 (vopratelimab) requires initial T cell priming and Fc cross-linking for optimal T cell activation and anti-tumor immunity in preclinical models
,Hanson, A;Elpek, K;Duong, E;Shallberg, L;Fan, M;Johnson, C;Wallace, M;Mabry, GR;Sazinsky, S;Pepper, L;Shu, CJ;Sathyanarayanan, S;Zuerndorfer, S;Simpson, T;Gostissa, M;Briskin, M;Law, D;Michaelson, J;Harvey, CJ;,
PLoS ONE
,PubMed ID 32970735
[ICOS]
|
Inclusion of Dap10 or 4-1BB costimulation domains in the chPD1 receptor enhances anti-tumor efficacy of T cells in murine models of lymphoma and melanoma
,Kintz, H;Nylen, E;Barber, A;,
Cell. Immunol.
,PubMed ID 32106933
[ICOS]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC204698 | Icos (untagged) - Mouse inducible T-cell co-stimulator (Icos), (10ug) |
CNY 2,000.00 |
|
MG202040 | Icos (tGFP-tagged) - Mouse inducible T-cell co-stimulator (Icos) |
CNY 2,850.00 |
|
MR202040L3 | Lenti ORF clone of Icos (Myc-DDK-tagged) - Mouse inducible T-cell co-stimulator (Icos) |
CNY 4,750.00 |
|
MR202040L4 | Lenti ORF clone of Icos (mGFP-tagged) - Mouse inducible T-cell co-stimulator (Icos) |
CNY 4,750.00 |