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Kit (NM_001122733) Mouse Tagged ORF Clone
CAT＃: MG227469
- TrueORF®
Kit (tGFP-tagged) - Mouse kit oncogene (Kit) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001122733" in other vectors (4)
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CNY 12,352.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | belly; Bs; c-KI; c-KIT; CD117; Fdc; Gsfs; Gsfsco1; Gsfsco5; Gsfsow3; SC; SCO1; SCO5; SO; SOW3; Ssm; Tr-k; Tr-kit; W |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227469 representing NM_001122733
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAGGCGCTCGCGGCGCCTGGGATCTGCTCTGCGTCCTGTTGGTCCTGCTCCGTGGCCAGACAGCCA CGTCTCAGCCATCTGCAAGTCCAGGGGAGCCGTCTCCGCCATCCATCCATCCAGCACAATCAGAGTTAAT AGTTGAAGCTGGCGACACCCTCAGCCTGACGTGCATTGATCCCGACTTTGTCAGATGGACTTTCAAGACC TATTTCAATGAAATGGTTGAGAATAAAAAAAATGAATGGATCCAGGAAAAAGCCGAGGCCACTCGCACGG GCACATACACGTGCAGCAACAGCAATGGCCTCACGAGTTCTATTTACGTGTTTGTTAGAGATCCTGCCAA ACTTTTCCTGGTTGGCCTTCCCTTGTTTGGCAAAGAAGACAGCGACGCGCTGGTCCGCTGCCCTCTGACA GACCCACAGGTGTCCAATTATTCCCTCATCGAGTGTGATGGGAAATCTCTCCCCACGGACCTGACGTTTG TCCCAAACCCCAAGGCTGGCATCACCATCAAAAACGTGAAGCGCGCCTACCACCGGCTCTGTGTCCGCTG TGCTGCTCAGCGTGACGGTACATGGCTGCATTCTGACAAATTCACCCTCAAAGTGCGGGCAGCCATCAAG GCTATCCCTGTTGTGTCTGTGCCTGAAACAAGTCACCTCCTTAAGAAAGGGGACACATTTACGGTGGTGT GCACCATAAAAGATGTGTCTACATCCGTGAACTCCATGTGGCTAAAGATGAACCCTCAGCCTCAGCACAT AGCCCAGGTAAAGCACAATAGCTGGCACCGGGGTGACTTCAATTATGAACGCCAGGAGACGCTGACTATC AGCTCGGCAAGAGTTGACGATTCTGGAGTGTTCATGTGTTATGCCAATAATACTTTTGGATCAGCAAATG TCACAACAACCTTGAAAGTAGTAGAAAAAGGATTCATCAACATCTCCCCTGTGAAGAACACTACAGTATT TGTAACCGATGGAGAAAACGTAGATTTGGTTGTTGAATACGAGGCCTACCCCAAACCCGAGCACCAGCAG TGGATATATATGAACAGGACCTCGGCTAACAAAGGGAAGGATTATGTCAAATCTGATAACAAAAGCAACA TCAGATATGTGAACCAACTTCGCCTGACCAGATTAAAAGGCACAGAAGGAGGCACTTATACCTTTCTGGT GTCCAACTCTGATGCCAGTGCTTCCGTGACATTCAACGTTTACGTGAACACAAAACCAGAAATCCTGACG TACGACAGGCTCATAAATGGCATGCTCCAGTGTGTGGCAGAGGGATTCCCGGAGCCCACAATAGATTGGT ATTTTTGTACAGGAGCAGAGCAAAGGTGTACCACTCCTGTCTCACCAGTGGACGTACAGGTCCAGAATGT ATCTGTGTCACCATTTGGAAAACTGGTGGTTCAGAGTTCCATAGACTCCAGCGTCTTCCGGCACAACGGC ACGGTGGAGTGTAAGGCCTCCAACGATGTGGGCAAGAGTTCCGCCTTCTTTAACTTTGCATTTAAAGGTA ACAACAAAGAGCAAATCCAGGCCCACACTCTGTTCACGCCGCTGCTCATTGGCTTTGTGGTCGCAGCTGG CGCGATGGGGATCATTGTGATGGTGCTCACCTACAAATATTTGCAGAAACCCATGTATGAAGTACAATGG AAGGTTGTCGAGGAGATAAATGGAAACAATTATGTTTACATAGACCCGACGCAACTTCCTTATGATCACA AATGGGAGTTTCCCAGAAACAGGCTGAGTTTTGGAAAGACATTGGGAGCTGGTGCCTTCGGGAAGGTCGT TGAGGCCACTGCATATGGCTTGATTAAGTCGGATGCTGCCATGACAGTTGCCGTGAAGATGCTCAAACCA AGTGCCCATTTAACAGAAAGAGAGGCCCTAATGTCGGAACTGAAGGTCCTGAGCTACCTGGGCAATCACA TGAATATTGTGAACCTGCTTGGCGCATGCACGGTGGGAGGGCCCACCCTGGTCATTACAGAATATTGTTG CTATGGTGATCTTTTGAATTTTTTGAGAAGGAAGCGTGACTCGTTTATTTTCTCAAAGCAAGAAGAGCAG GCAGAAGCGGCACTTTATAAGAACCTTCTGCACTCAACGGAGCCTTCCTGTGACAGTTCAAATGAATATA TGGACATGAAGCCTGGCGTTTCCTACGTGGTGCCAACCAAGACAGACAAGAGGAGATCCGCAAGAATAGA CTCGTACATAGAAAGAGACGTGACTCCTGCCATCATGGAAGATGACGAGCTGGCTCTGGACCTGGATGAT TTGCTGAGCTTCTCCTACCAGGTGGCCAAGGGCATGGCGTTCCTCGCCTCCAAGAATTGTATTCACAGAG ATTTGGCAGCCAGGAATATCCTCCTCACTCACGGGCGGATCACAAAGATTTGCGATTTCGGGCTAGCCAG AGACATCAGGAATGATTCGAATTACGTGGTCAAAGGAAATGCACGACTGCCCGTGAAGTGGATGGCACCA GAGAGCATTTTCAGCTGCGTGTACACATTTGAAAGTGATGTCTGGTCCTATGGGATTTTCCTCTGGGAGC TCTTCTCCTTAGGAAGCAGCCCCTACCCAGGGATGCCGGTCGACTCCAAGTTCTACAAGATGATCAAGGA AGGCTTCCGGATGGTCAGCCCGGAGCACGCGCCTGCCGAAATGTATGACGTCATGAAGACTTGCTGGGAC GCTGACCCCTTGAAAAGGCCAACATTCAAGCAGGTTGTCCAACTTATTGAGAAGCAGATCTCGGACAGCA CCAAGCACATTTACTCCAACTTGGCAAACTGCAACCCCAACCCAGAGAACCCCGTGGTGGTGGACCATTC CGTGAGGGTCAACTCGGTGGGCAGCAGCGCCTCTTCTACGCAGCCCCTGCTCGTGCACGAAGATGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227469 representing NM_001122733
Red=Cloning site Green=Tags(s) MRGARGAWDLLCVLLVLLRGQTATSQPSASPGEPSPPSIHPAQSELIVEAGDTLSLTCIDPDFVRWTFKT YFNEMVENKKNEWIQEKAEATRTGTYTCSNSNGLTSSIYVFVRDPAKLFLVGLPLFGKEDSDALVRCPLT DPQVSNYSLIECDGKSLPTDLTFVPNPKAGITIKNVKRAYHRLCVRCAAQRDGTWLHSDKFTLKVRAAIK AIPVVSVPETSHLLKKGDTFTVVCTIKDVSTSVNSMWLKMNPQPQHIAQVKHNSWHRGDFNYERQETLTI SSARVDDSGVFMCYANNTFGSANVTTTLKVVEKGFINISPVKNTTVFVTDGENVDLVVEYEAYPKPEHQQ WIYMNRTSANKGKDYVKSDNKSNIRYVNQLRLTRLKGTEGGTYTFLVSNSDASASVTFNVYVNTKPEILT YDRLINGMLQCVAEGFPEPTIDWYFCTGAEQRCTTPVSPVDVQVQNVSVSPFGKLVVQSSIDSSVFRHNG TVECKASNDVGKSSAFFNFAFKGNNKEQIQAHTLFTPLLIGFVVAAGAMGIIVMVLTYKYLQKPMYEVQW KVVEEINGNNYVYIDPTQLPYDHKWEFPRNRLSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKP SAHLTEREALMSELKVLSYLGNHMNIVNLLGACTVGGPTLVITEYCCYGDLLNFLRRKRDSFIFSKQEEQ AEAALYKNLLHSTEPSCDSSNEYMDMKPGVSYVVPTKTDKRRSARIDSYIERDVTPAIMEDDELALDLDD LLSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIRNDSNYVVKGNARLPVKWMAP ESIFSCVYTFESDVWSYGIFLWELFSLGSSPYPGMPVDSKFYKMIKEGFRMVSPEHAPAEMYDVMKTCWD ADPLKRPTFKQVVQLIEKQISDSTKHIYSNLANCNPNPENPVVVDHSVRVNSVGSSASSTQPLLVHEDA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001122733 |
| ORF Size | 2937 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001122733.1, NP_001116205.1 |
| RefSeq Size | 5205 bp |
| RefSeq ORF | 2940 bp |
| Locus ID | 16590 |
| UniProt ID | P05532 |
| Gene Summary | The c-Kit proto-oncogene is the cellular homolog of the transforming gene of a feline retrovirus (v-Kit). The c-kit protein includes characteristics of a protein kinase transmembrane receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222941 | Kit (untagged) - Mouse kit oncogene (Kit), transcript variant 1, (10ug) |
CNY 10,768.00 |
|
| MR227469 | Kit (Myc-DDK-tagged) - Mouse kit oncogene (Kit), transcript variant 1 |
CNY 10,752.00 |
|
| MR227469L3 | Lenti ORF clone of Kit (Myc-DDK-tagged) - Mouse kit oncogene (Kit), transcript variant 1 |
CNY 11,690.00 |
|
| MR227469L4 | Lenti ORF clone of Kit (mGFP-tagged) - Mouse kit oncogene (Kit), transcript variant 1 |
CNY 11,690.00 |
