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Abcg8 (NM_026180) Mouse Tagged ORF Clone
CAT＃: MG227387
- TrueORF®
Abcg8 (tGFP-tagged) - Mouse ATP-binding cassette sub-family G (WHITE) member 8 (Abcg8), (10ug)
ORF Plasmid: DDK
"NM_026180" in other vectors (4)
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CNY 7,410.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1300003C16Rik; AI114946; sterolin-2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227387 representing NM_026180
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAGAAAACCAAAGAAGAGACCCAGCTGTGGAATGGGACTGTACTTCAGGATGCTTCGGGCCTCC AGGACAGCTTGTTCTCCTCGGAAAGTGACAACAGTCTGTACTTCACCTACAGTGGTCAGTCCAACACTCT GGAGGTCAGAGATCTCACCTACCAGGTGGACATCGCCTCTCAGGTGCCTTGGTTTGAGCAGCTGGCTCAG TTCAAGATACCCTGGAGGTCTCATAGCAGCCAAGACTCCTGTGAGCTGGGCATCCGAAATCTAAGCTTCA AAGTGAGGAGTGGACAGATGCTGGCCATCATAGGGAGCTCAGGCTGCGGGAGAGCCTCACTACTCGACGT GATCACAGGCAGAGGCCACGGTGGCAAGATGAAATCAGGACAAATTTGGATAAATGGGCAACCCAGTACG CCTCAGCTGGTGAGGAAGTGCGTTGCGCATGTGCGGCAGCATGACCAACTGCTGCCCAACCTGACCGTCA GAGAGACCCTGGCTTTCATTGCCCAGATGCGCCTGCCCAGGACCTTCTCCCAGGCCCAGCGTGACAAACG GGTGGAAGACGTAATCGCCGAGCTGCGGCTGCGGCAGTGCGCCAACACCAGAGTGGGCAACACGTATGTA CGTGGGGTGTCCGGGGGTGAGCGCCGACGAGTGAGCATTGGGGTGCAGCTCCTGTGGAACCCAGGAATCC TCATTCTGGATGAACCCACTTCTGGCCTCGACAGCTTCACAGCCCACAATCTGGTGACAACCTTGTCCCG CCTGGCCAAGGGCAACAGGCTGGTGCTCATCTCCCTCCACCAGCCTCGCTCTGACATCTTCAGGCTATTT GACCTGGTCCTTCTGATGACATCTGGCACCCCTATCTACCTGGGGGCGGCGCAGCAAATGGTGCAGTACT TCACATCCATTGGCCACCCTTGTCCTCGCTATAGCAACCCTGCGGACTTCTACGTGGACTTGACCAGCAT CGACAGACGCAGCAAAGAACGGGAGGTGGCCACCGTGGAGAAGGCACAGTCTCTTGCAGCCCTGTTCCTA GAAAAAGTACAAGGCTTTGATGACTTTCTGTGGAAAGCTGAGGCAAAGGAACTCAACACAAGCACCCACA CAGTCAGCCTGACCCTCACACAGGACACTGACTGTGGGACTGCTGTTGAGCTGCCCGGGATGATAGAGCA GTTTTCCACCCTGATCCGTCGTCAGATTTCCAATGACTTCCGGGACCTGCCCACGCTGCTCATTCATGGG TCGGAAGCCTGCCTGATGTCCCTCATCATTGGCTTCCTTTACTACGGCCATGGGGCCAAGCAGCTCTCCT TCATGGACACAGCAGCCCTCCTCTTCATGATAGGGGCGCTCATTCCTTTCAATGTCATCCTGGATGTCGT CTCCAAATGTCACTCGGAGAGGTCAATGCTGTACTATGAGCTGGAAGACGGGCTGTACACTGCTGGTCCT TATTTCTTTGCCAAGATCCTAGGAGAATTGCCGGAGCACTGTGCCTACGTCATCATCTACGCGATGCCCA TCTACTGGCTGACAAACCTGCGGCCCGTGCCTGAGCTCTTCCTTCTACACTTCCTGCTCGTGTGGTTGGT GGTCTTCTGCTGCAGGACCATGGCCCTGGCTGCCTCTGCCATGCTGCCCACCTTCCACATGTCCTCCTTC TTCTGCAATGCCCTCTACAACTCCTTCTACCTTACTGCCGGCTTCATGATAAACTTGGACAACCTGTGGA TAGTGCCTGCATGGATCTCCAAGCTGTCGTTCCTCCGGTGGTGCTTCTCGGGGCTGATGCAGATTCAATT TAATGGACACCTTTACACCACACAAATCGGCAACTTCACCTTCTCCATCCTCGGAGACACGATGATCAGT GCCATGGACCTGAACTCGCATCCACTCTATGCGATCTACCTCATTGTCATCGGCATCAGCTACGGCTTCC TGTTCCTGTACTATCTATCCTTGAAGCTCATCAAACAGAAGTCAATTCAAGACTGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227387 representing NM_026180
Red=Cloning site Green=Tags(s) MAEKTKEETQLWNGTVLQDASGLQDSLFSSESDNSLYFTYSGQSNTLEVRDLTYQVDIASQVPWFEQLAQ FKIPWRSHSSQDSCELGIRNLSFKVRSGQMLAIIGSSGCGRASLLDVITGRGHGGKMKSGQIWINGQPST PQLVRKCVAHVRQHDQLLPNLTVRETLAFIAQMRLPRTFSQAQRDKRVEDVIAELRLRQCANTRVGNTYV RGVSGGERRRVSIGVQLLWNPGILILDEPTSGLDSFTAHNLVTTLSRLAKGNRLVLISLHQPRSDIFRLF DLVLLMTSGTPIYLGAAQQMVQYFTSIGHPCPRYSNPADFYVDLTSIDRRSKEREVATVEKAQSLAALFL EKVQGFDDFLWKAEAKELNTSTHTVSLTLTQDTDCGTAVELPGMIEQFSTLIRRQISNDFRDLPTLLIHG SEACLMSLIIGFLYYGHGAKQLSFMDTAALLFMIGALIPFNVILDVVSKCHSERSMLYYELEDGLYTAGP YFFAKILGELPEHCAYVIIYAMPIYWLTNLRPVPELFLLHFLLVWLVVFCCRTMALAASAMLPTFHMSSF FCNALYNSFYLTAGFMINLDNLWIVPAWISKLSFLRWCFSGLMQIQFNGHLYTTQIGNFTFSILGDTMIS AMDLNSHPLYAIYLIVIGISYGFLFLYYLSLKLIKQKSIQDW TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_026180 |
| ORF Size | 2019 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_026180.2 |
| RefSeq Size | 3674 bp |
| RefSeq ORF | 2022 bp |
| Locus ID | 67470 |
| UniProt ID | Q9DBM0 |
| Gene Summary | ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg(2+)- and ATP-dependent sterol transport across the cell membrane (PubMed:16352607, PubMed:16867993, PubMed:18402465). Plays an essential role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile (PubMed:12444248, PubMed:14504269, PubMed:14657202, PubMed:25378657). Plays an important role in preventing the accumulation of dietary plant sterols in the body (PubMed:12444248, PubMed:14657202). Required for normal sterol homeostasis (PubMed:12444248, PubMed:14657202). The heterodimer with ABCG5 has ATPase activity (PubMed:16352607, PubMed:16867993).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220347 | Abcg8 (untagged) - Mouse ATP-binding cassette, sub-family G (WHITE), member 8 (Abcg8), (10ug) |
CNY 8,650.00 |
|
| MR227387 | Abcg8 (Myc-DDK-tagged) - Mouse ATP-binding cassette, sub-family G (WHITE), member 8 (Abcg8) |
CNY 7,392.00 |
|
| MR227387L3 | Lenti ORF clone of Abcg8 (Myc-DDK-tagged) - Mouse ATP-binding cassette, sub-family G (WHITE), member 8 (Abcg8) |
CNY 8,650.00 |
|
| MR227387L4 | Lenti ORF clone of Abcg8 (mGFP-tagged) - Mouse ATP-binding cassette, sub-family G (WHITE), member 8 (Abcg8) |
CNY 8,650.00 |
