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Mef2c (NM_025282) Mouse Tagged ORF Clone
CAT＃: MG226865
- TrueORF®
Mef2c (tGFP-tagged) - Mouse myocyte enhancer factor 2C (Mef2c) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_025282" in other vectors (4)
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CNY 3,710.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 5430401D19Rik; 9930028G15Rik; AV011172; Mef2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226865 representing NM_025282
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAGAAAAAAGATTCAGATTACGAGGATAATGGATGAGCGTAACAGACAGGTGACTTTTACGAAGA GGAAATTTGGATTGATGAAGAAGGCTTATGAGCTGAGCGTGCTGTGCGACTGTGAGATTGCACTGATCAT CTTCAACAGCACCAACAAGCTGTTCCAGTACGCCAGCACTGACATGGATAAGGTGTTGCTCAAGTACACC GAGTACAACGAGCCGCACGAGAGCCGGACAAACTCAGACATTGTGGAGGCATTGAACAAGAAAGAAAACA AAGGCTCTGAAAGCCCCGATCCTGACTCCTCTTATGCACTCACCCCACGCACTGAAGAAAAATACAAAAA AATTAATGAAGAATTTGATAATATGATCAAGAGTCATAAAATTCCTGCTGTTCCACCTCCCAGCTTTGAG ATGCCAGTTACCATCCCAGTGTCCAGCCATAACAGTTTGGTGTACAGCAATCCTGTCAGCACACTGGGAA ACCCCAATCTTCTGCCACTGGCCCACCCGTCTCTGCAGAGGAATAGTATGTCTCCTGGTGTAACACATAG ACCTCCAAGTGCAGGTAACACAGGCGGTCTGATGGGCGGAGATCTGACATCCGGTGCAGGCACCAGCGCA GGGAATGGATACGGCAACCCCCGGAACTCACCAGGCCTGCTGGTCTCACCTGGTAACCTGAACAAGAATA TACAAGCCAAATCTCCTCCCCCTATGAATCTAGGAATGAATAATCGTAAGCCAGATCTCCGCGTTCTTAT CCCACCTGGCAGCAAGAACACGATGCCATCAGTGAATCAAAGGATAAATAACTCCCAGTCGGCTCAGTCA TTGGCTACCCCGGTGGTTTCCGTAGCAACTCCTACTTTACCAGGACAAGGAATGGGAGGATATCCATCAG CCATTTCAACAACATATGGTACTGAGTACTCTCTGAGTAGCGCAGATCTGTCATCTCTGTCTGGCTTCAA CACTGCCAGTGCGCTCCACCTCGGCTCTGTAACTGGCTGGCAGCAGCAGCACCTACATAACATGCCGCCA TCTGCCCTCAGTCAGTTGGGAGACCGTACCACCACCCCTTCGAGATACCCACAACACACCACGCGCCACG AGGCGGGGAGGTCTCCTGTTGACAGCTTGAGCAGCTGTAGCAGTTCCTACGATGGGAGCGACCGAGAGGA TCACCGGAACGAATTCCACTCCCCCATTGGACTCACCAGACCTTCGCCGGACGAAAGGGAAAGTCCTTCA GTCAAGCGCATGCGACTCTCTGAAGGATGGGCAACA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226865 representing NM_025282
Red=Cloning site Green=Tags(s) MGRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFNSTNKLFQYASTDMDKVLLKYT EYNEPHESRTNSDIVEALNKKENKGSESPDPDSSYALTPRTEEKYKKINEEFDNMIKSHKIPAVPPPSFE MPVTIPVSSHNSLVYSNPVSTLGNPNLLPLAHPSLQRNSMSPGVTHRPPSAGNTGGLMGGDLTSGAGTSA GNGYGNPRNSPGLLVSPGNLNKNIQAKSPPPMNLGMNNRKPDLRVLIPPGSKNTMPSVNQRINNSQSAQS LATPVVSVATPTLPGQGMGGYPSAISTTYGTEYSLSSADLSSLSGFNTASALHLGSVTGWQQQHLHNMPP SALSQLGDRTTTPSRYPQHTTRHEAGRSPVDSLSSCSSSYDGSDREDHRNEFHSPIGLTRPSPDERESPS VKRMRLSEGWAT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025282 |
| ORF Size | 1296 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_025282.3, NP_079558.1 |
| RefSeq Size | 6325 bp |
| RefSeq ORF | 1299 bp |
| Locus ID | 17260 |
| UniProt ID | Q8CFN5 |
| Gene Summary | Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Enhances transcriptional activation mediated by SOX18 (PubMed:11554755). May also be involved in neurogenesis and in the development of cortical architecture. Isoforms that lack the repressor domain are more active than isoform 1 (By similarity). Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B-lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B-cells.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208956 | Mef2c (untagged) - Mouse myocyte enhancer factor 2C (Mef2c), transcript variant 2, (10ug) |
CNY 3,990.00 |
|
| MR226865 | Mef2c (Myc-DDK-tagged) - Mouse myocyte enhancer factor 2C (Mef2c), transcript variant 2 |
CNY 3,330.00 |
|
| MR226865L3 | Lenti ORF clone of Mef2c (Myc-DDK-tagged) - Mouse myocyte enhancer factor 2C (Mef2c), transcript variant 2 |
CNY 5,230.00 |
|
| MR226865L4 | Lenti ORF clone of Mef2c (mGFP-tagged) - Mouse myocyte enhancer factor 2C (Mef2c), transcript variant 2 |
CNY 5,230.00 |
