United States
Germany
Japan
United Kingdom
China
Rspo1 (NM_138683) Mouse Tagged ORF Clone
CAT＃: MG226539
- TrueORF®
Rspo1 (tGFP-tagged) - Mouse R-spondin homolog (Xenopus laevis) (Rspo1), (10ug)
ORF Plasmid: DDK
"NM_138683" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,200.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | R-spondin; Rspondin |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226539 representing NM_138683
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGCTTGGGCTGTGCGTGGTGGCCCTGGTTCTGAGCTGGACACACATCGCCGTGGGCAGCCGGGGGA TCAAGGGCAAGAGACAGAGGCGGATCAGTGCTGAGGGGAGCCAAGCCTGCGCCAAGGGCTGTGAGCTCTG TTCAGAAGTCAACGGTTGCCTCAAGTGCTCGCCCAAGCTCTTCATTCTGCTGGAGAGGAACGACATCCGC CAGGTGGGCGTCTGCCTGCCGTCCTGCCCACCTGGATACTTTGATGCCCGCAACCCCGACATGAACAAAT GCATCAAATGCAAGATCGAGCACTGTGAGGCCTGCTTCAGCCACAACTTCTGCACCAAGTGTCAGGAGGG CTTGTACTTACACAAGGGCCGCTGCTATCCAGCCTGCCCTGAGGGCTCTACAGCCGCTAACAGCACCATG GAGTGCGGCAGTCCTGCACAATGTGAAATGAGCGAGTGGTCCCCGTGGGGACCCTGCTCCAAGAAGAGGA AGCTGTGCGGTTTCCGGAAGGGATCGGAAGAGCGGACACGCAGAGTGCTCCATGCTCCCGGGGGAGACCA CACCACCTGCTCCGACACCAAAGAGACCCGCAAGTGTACCGTGCGCAGGACGCCCTGCCCAGAGGGGCAG AAGAGGAGGAAGGGGGGCCAGGGCCGGAGGGAGAATGCCAACAGGCATCCGGCCAGGAAGAACAGCAAGG AGCCGGGCTCCAACTCTCGGAGACACAAAGGGCAACAGCAGCCACAGCCAGGGACAACAGGGCCACTCAC ATCAGTAGGACCTACCTGGGCACAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226539 representing NM_138683
Red=Cloning site Green=Tags(s) MRLGLCVVALVLSWTHIAVGSRGIKGKRQRRISAEGSQACAKGCELCSEVNGCLKCSPKLFILLERNDIR QVGVCLPSCPPGYFDARNPDMNKCIKCKIEHCEACFSHNFCTKCQEGLYLHKGRCYPACPEGSTAANSTM ECGSPAQCEMSEWSPWGPCSKKRKLCGFRKGSEERTRRVLHAPGGDHTTCSDTKETRKCTVRRTPCPEGQ KRRKGGQGRRENANRHPARKNSKEPGSNSRRHKGQQQPQPGTTGPLTSVGPTWAQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138683 |
| ORF Size | 795 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_138683.2, NP_619624.2 |
| RefSeq Size | 1834 bp |
| RefSeq ORF | 798 bp |
| Locus ID | 192199 |
| UniProt ID | Q9Z132 |
| Gene Summary | Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes (PubMed:21693646). Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination (By similarity). Regulates Wnt signaling by antagonizing DKK1/KREM1-mediated internalization of LRP6 through an interaction with KREM1 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC212441 | Rspo1 (untagged) - Mouse R-spondin homolog (Xenopus laevis) (Rspo1), (10ug) |
CNY 3,600.00 |
|
| MR226539 | Rspo1 (Myc-DDK-tagged) - Mouse R-spondin homolog (Xenopus laevis) (Rspo1) |
CNY 3,600.00 |
|
| MR226539L3 | Lenti ORF clone of Rspo1 (Myc-DDK-tagged) - Mouse R-spondin homolog (Xenopus laevis) (Rspo1) |
CNY 5,890.00 |
|
| MR226539L4 | Lenti ORF clone of Rspo1 (mGFP-tagged) - Mouse R-spondin homolog (Xenopus laevis) (Rspo1) |
CNY 6,000.00 |
