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Pik3r1 (NM_001024955) Mouse Tagged ORF Clone
CAT＃: MG226192
- TrueORF®
Pik3r1 (tGFP-tagged) - Mouse phosphatidylinositol 3-kinase regulatory subunit polypeptide 1 (p85 alpha) (Pik3r1) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001024955" in other vectors (4)
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CNY 7,456.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | p50alpha; p55alpha; p85alpha; PI3K |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226192 representing NM_001024955
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACACCACGGTTTGGACTATGGAAGACCTGGACTTAGAGTGTGCCAAGACAGATATAAACTGTGGCA CAGACTTGGTGTTTTATATAGAAATGGATCCACCAGCACTGCCCCCCAAACCACCCAAGCCCACTACTGT AGCCAACAACAGCATGAACAACAATATGTCCTTGCAGGATGCTGAATGGTACTGGGGAGACATCTCAAGG GAAGAAGTGAATGAAAAACTCCGAGACACTGCTGATGGGACCTTTTTGGTACGAGACGCATCTACTAAAA TGCACGGCGATTACACTCTTACACTAAGGAAAGGAGGAAATAACAAATTAATCAAAATCTTTCACCGTGA TGGAAAATATGGCTTCTCTGATCCATTAACCTTCAACTCTGTGGTTGAGTTAATAAACCACTACCGGAAT GAGTCTTTAGCTCAGTACAACCCCAAGCTGGATGTGAAGTTGCTCTACCCAGTGTCCAAATACCAGCAGG ATCAAGTTGTCAAAGAAGATAATATTGAAGCTGTAGGGAAAAAATTACATGAATATAATACTCAATTTCA AGAAAAAAGTCGGGAATATGATAGATTATATGAGGAGTACACCCGTACTTCCCAGGAAATCCAAATGAAA AGAACGGCTATCGAAGCATTTAATGAAACCATAAAAATATTTGAAGAACAATGCCAAACCCAGGAGCGGT ACAGCAAAGAATACATAGAGAAGTTTAAACGCGAAGGCAACGAGAAAGAAATTCAAAGGATTATGCATAA CCATGATAAGCTGAAGTCGCGTATCAGTGAGATCATTGACAGTAGGAGGAGGTTGGAAGAAGACTTGAAG AAGCAGGCAGCTGAGTACCGAGAGATCGACAAACGCATGAACAGTATTAAGCCGGACCTCATCCAGTTGA GAAAGACAAGAGACCAATACTTGATGTGGCTGACGCAGAAAGGTGTGCGGCAGAAGAAGCTGAACGAGTG GCTGGGGAATGAAAATACCGAAGATCAATACTCCCTGGTAGAAGATGATGAGGATTTGCCCCACCATGAC GAGAAGACGTGGAATGTCGGGAGCAGCAACCGAAACAAAGCGGAGAACCTATTGCGAGGGAAGCGAGACG GCACTTTCCTTGTCCGGGAGAGCAGTAAGCAGGGCTGCTATGCCTGCTCCGTAGTGGTAGACGGCGAAGT CAAGCATTGCGTCATTAACAAGACTGCCACCGGCTATGGCTTTGCCGAGCCCTACAACCTGTACAGCTCC CTGAAGGAGCTGGTGCTACATTATCAACACACCTCCCTCGTGCAGCACAATGACTCCCTCAATGTCACAC TAGCATACCCAGTATATGCACAACAGAGGCGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226192 representing NM_001024955
Red=Cloning site Green=Tags(s) MYTTVWTMEDLDLECAKTDINCGTDLVFYIEMDPPALPPKPPKPTTVANNSMNNNMSLQDAEWYWGDISR EEVNEKLRDTADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGFSDPLTFNSVVELINHYRN ESLAQYNPKLDVKLLYPVSKYQQDQVVKEDNIEAVGKKLHEYNTQFQEKSREYDRLYEEYTRTSQEIQMK RTAIEAFNETIKIFEEQCQTQERYSKEYIEKFKREGNEKEIQRIMHNHDKLKSRISEIIDSRRRLEEDLK KQAAEYREIDKRMNSIKPDLIQLRKTRDQYLMWLTQKGVRQKKLNEWLGNENTEDQYSLVEDDEDLPHHD EKTWNVGSSNRNKAENLLRGKRDGTFLVRESSKQGCYACSVVVDGEVKHCVINKTATGYGFAEPYNLYSS LKELVLHYQHTSLVQHNDSLNVTLAYPVYAQQRR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001024955 |
| ORF Size | 1362 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001024955.2, NP_001020126.1 |
| RefSeq Size | 5843 bp |
| RefSeq ORF | 1365 bp |
| Locus ID | 18708 |
| Gene Summary | Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (By similarity). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348926).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216127 | Pik3r1 (untagged) - Mouse phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) (Pik3r1), transcript variant 1, (10ug) |
CNY 5,990.00 |
|
| MR226192 | Pik3r1 (Myc-DDK-tagged) - Mouse phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) (Pik3r1), transcript variant 1 |
CNY 4,560.00 |
|
| MR226192L3 | Lenti ORF clone of Pik3r1 (Myc-DDK-tagged) - Mouse phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) (Pik3r1), transcript variant 1 |
CNY 6,460.00 |
|
| MR226192L4 | Lenti ORF clone of Pik3r1 (mGFP-tagged) - Mouse phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) (Pik3r1), transcript variant 1 |
CNY 6,460.00 |
