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Slc14a1 (NM_001171010) Mouse Tagged ORF Clone
CAT＃: MG218377
- TrueORF®
Slc14a1 (tGFP-tagged) - Mouse solute carrier family 14 (urea transporter) member 1 (Slc14a1) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001171010" in other vectors (4)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2610507K20Rik; 3021401A05Rik; UT-B; Utb1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG218377 representing NM_001171010
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGGACAATCTTTGACTGGTGGCACTGATGACGCCCATCACGGTCCTCTTTGGATAGACCCTTTCG GAAATAGAGGCGATAAGGCAGCGCCAGAAGGCTTCAGACGTCTAAGCTTGGCCCTGGCACAAAGGTGGCG AGAACAGGAGCCAGAGGAAGAGATAGCCATGGAAGATAGTCCCACCATGGTTAAAGTAGACCGGGGTGAA AACCAGATTTTATCATGCCGCGGGAGAAGGTGTGGCTTCAAAGTACTTGGCTACGTCACGGGTGACATGA AGGAATTCGCCAACTGGCTGAAAGACAAACCCGTGGTGCTCCAGTTCATGGACTGGATACTTCGTGGCAT ATCCCAGGTGGTGTTTGTCAGCAACCCCATCAGTGGAATCCTGATTCTGGTGGGACTTCTGGTCCAGAAC CCCTGGTGGGCTCTCTGTGGCTGTGTAGGAACTGTGGTCTCCACTCTGACAGCCCTCTTGCTTAGCCAAG ACAGATCGGCGATAGCAGCGGGGCTCCAAGGTTACAATGCCACCCTGGTAGGCATCCTCATGGCTGTCTT CTCAAACAAGGGCGACTATTTCTGGTGGCTGATATTCCCTGTATCTGCTATGTCTATGACTTGCCCGGTT TTCTCGAGCGCGTTGAGCTCCGTGCTCAGCAAGTGGGACCTGCCCGTCTTCACTCTCCCTTTCAACATGG CGTTGTCGATGTACCTGTCAGCCACAGGACACTACAATACGTTTTTCCCAAGTAAACTCTTCACACCTGT CAGCTCCGTGCCCAACATCACGTGGTCTGAGCTCAGCGCCCTGGAGCTATTGAAGTCTCTTCCGGTGGGA GTCGGTCAGATATATGGCTGTGACAACCCGTGGACAGGCGGCATTTTCCTATGTGCTATCCTGCTCTCCT CCCCACTCATGTGCCTGCACGCTGCTATTGGATCGTTGCTGGGTGTCATCGCGGGACTCAGTCTTGCAGC TCCATTTGAAGACATCTACTTTGGGCTCTGGGGTTTCAACAGCTCTCTGGCCTGCATTGCAATTGGAGGG ATGTTCATGGCACTCACCTGGCAGACCCACCTCCTGGCTCTTGCCTGTGCCCTGTTCACTGCCTACTTCG GAGCCTGTATGGCACACCTGATGGCTGTGGTTCACCTGCCAGCTTGTACCTGGTCCTTCTGTTTGGCCAC ACTACTCTTTCTCTTGCTGACCACGAAAAATCCCAACATCTACAGGATGCCCCTCAGCAAAGTTACCTAC TCTGAGGAGAACCGCATCTTCTACCTCCAAAACAAGAAAAGGATGGTTGAAAGCCCCCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218377 representing NM_001171010
Red=Cloning site Green=Tags(s) MNGQSLTGGTDDAHHGPLWIDPFGNRGDKAAPEGFRRLSLALAQRWREQEPEEEIAMEDSPTMVKVDRGE NQILSCRGRRCGFKVLGYVTGDMKEFANWLKDKPVVLQFMDWILRGISQVVFVSNPISGILILVGLLVQN PWWALCGCVGTVVSTLTALLLSQDRSAIAAGLQGYNATLVGILMAVFSNKGDYFWWLIFPVSAMSMTCPV FSSALSSVLSKWDLPVFTLPFNMALSMYLSATGHYNTFFPSKLFTPVSSVPNITWSELSALELLKSLPVG VGQIYGCDNPWTGGIFLCAILLSSPLMCLHAAIGSLLGVIAGLSLAAPFEDIYFGLWGFNSSLACIAIGG MFMALTWQTHLLALACALFTAYFGACMAHLMAVVHLPACTWSFCLATLLFLLLTTKNPNIYRMPLSKVTY SEENRIFYLQNKKRMVESPL TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001171010 |
| ORF Size | 1320 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001171010.1, NP_001164481.1 |
| RefSeq Size | 3880 bp |
| RefSeq ORF | 1323 bp |
| Locus ID | 108052 |
| UniProt ID | Q8VHL0 |
| Gene Summary | Urea channel that facilitates transmembrane urea transport down a concentration gradient. A constriction of the transmembrane channel functions as selectivity filter through which urea is expected to pass in dehydrated form. The rate of urea conduction is increased by hypotonic stress. Plays an important role in the kidney medulla collecting ducts, where it allows rapid equilibration between the lumen of the collecting ducts and the interstitium, and thereby prevents water loss driven by the high concentration of urea in the urine. Facilitates urea transport across erythrocyte membranes. May also play a role in transmembrane water transport, possibly by indirect means.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC215854 | Slc14a1 (untagged) - Mouse solute carrier family 14 (urea transporter), member 1 (Slc14a1), transcript variant 1, (10ug) |
CNY 5,700.00 |
|
| MR218377 | Slc14a1 (Myc-DDK-tagged) - Mouse solute carrier family 14 (urea transporter), member 1 (Slc14a1), transcript variant 1 |
CNY 3,330.00 |
|
| MR218377L3 | Lenti ORF clone of Slc14a1 (Myc-DDK-tagged) - Mouse solute carrier family 14 (urea transporter), member 1 (Slc14a1), transcript variant 1 |
CNY 5,230.00 |
|
| MR218377L4 | Lenti ORF clone of Slc14a1 (mGFP-tagged) - Mouse solute carrier family 14 (urea transporter), member 1 (Slc14a1), transcript variant 1 |
CNY 5,230.00 |
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