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Ppm1k (NM_175523) Mouse Tagged ORF Clone
CAT＃: MG217753
- TrueORF®
Ppm1k (tGFP-tagged) - Mouse protein phosphatase 1K (PP2C domain containing) (Ppm1k) nuclear gene encoding mitochondrial protein, (10ug)
ORF Plasmid: DDK
"NM_175523" in other vectors (4)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2900063A19Rik; A930026L03Rik; PP2Cm |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG217753 representing NM_175523
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTATCAGCGGCCTTCATTACTTTGTTGAGAAGTGGTGGGAATCAGGTGAAGAAGAGAGTGTTGTTAA GCTCCATCCTCCTGCAGGACCACAGGCAGGCGACTCCTGCCTGCTACTTCTCCACTTCAGAGGCCAGGTG TTCTCGGTTTGATCCAGATGGAAGTGGGCAGCCAGCCACTTGGGACAATTTTGGGATCTGGGATAACCGC ATTGATGAGCCAATTCTGCTGCCACCTAGCATCAAGTACGGCAAACCAATTCCCAAAATTAGCCTGGAGA ACGTGGGCTGTGCCTCTCTCATTGGCAAACGGAAAGAAAACGAAGATCGATTTGGGTTTGCACAGCTGAC AGAAGAGGTGCTATACTTCGCAGTCTATGATGGACATGGGGGCCCTGCAGCCGCTGACTTCTGTCACACA CACATGGAGAAATGTGTTATGGATTTGCTTCCTCGGGAGAAAGACTTGGAAACTGTCCTGACCTTGGCCT TTCTAGAAATAGATAAAGCCTTCGCGAGTTATGCCCACCTGTCTGCAGATGCAAGCCTCCTGACCTCTGG GACTACTGCAACAGTAGCCCTGTTGAGAGATGGTGTTGAACTGGTGGTAGCCAGTGTTGGAGACAGCCGG GCTCTTTTGTGTAGAAAAGGAAAACCCATGAAGCTGACCACTGACCATACCCCAGAAAGAAAAGATGAGA AAGAAAGGATCAAGAAATTCGGTGGGTTTGTAGCTTGGAATAGTTTGGGACAGCCCCATGTAAATGGCAG ACTTGCAATGACAAGGAGTATCGGAGATTTGGATCTTAAAGCCAGTGGGGTAATTGCAGAACCTGAGACA ACAAGGATTAAGCTCTACCATGCTGACGACAGTTTCCTGGTCCTTACCACAGATGGGATTAACTTCATGG TGAATAGTCAAGAGATCTGCGACTTTGTCAACCAGTGCCACGATCCTAAAGAAGCAGCTCATTCGGTGAC TGAGCAGGCAATACAGTACGGTACCGAAGACAACAGCACTGCAGTAGTGGTGCCCTTTGGTGCTTGGGGA AAATACAAGAACTCTGAAATTACCTTCTCATTCAGCAGAAGCTTTGCCTCCAGTGGGAGATGGGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217753 representing NM_175523
Red=Cloning site Green=Tags(s) MLSAAFITLLRSGGNQVKKRVLLSSILLQDHRQATPACYFSTSEARCSRFDPDGSGQPATWDNFGIWDNR IDEPILLPPSIKYGKPIPKISLENVGCASLIGKRKENEDRFGFAQLTEEVLYFAVYDGHGGPAAADFCHT HMEKCVMDLLPREKDLETVLTLAFLEIDKAFASYAHLSADASLLTSGTTATVALLRDGVELVVASVGDSR ALLCRKGKPMKLTTDHTPERKDEKERIKKFGGFVAWNSLGQPHVNGRLAMTRSIGDLDLKASGVIAEPET TRIKLYHADDSFLVLTTDGINFMVNSQEICDFVNQCHDPKEAAHSVTEQAIQYGTEDNSTAVVVPFGAWG KYKNSEITFSFSRSFASSGRWA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175523 |
| ORF Size | 1116 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175523.5 |
| RefSeq Size | 5560 bp |
| RefSeq ORF | 1119 bp |
| Locus ID | 243382 |
| UniProt ID | Q8BXN7 |
| Gene Summary | Regulates the mitochondrial permeability transition pore and is essential for cellular survival and development.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC213167 | Ppm1k (untagged) - Mouse protein phosphatase 1K (PP2C domain containing) (Ppm1k), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 3,990.00 |
|
| MR217753 | Ppm1k (Myc-DDK-tagged) - Mouse protein phosphatase 1K (PP2C domain containing) (Ppm1k), nuclear gene encoding mitochondrial protein |
CNY 3,330.00 |
|
| MR217753L3 | Lenti ORF clone of Ppm1k (Myc-DDK-tagged) - Mouse protein phosphatase 1K (PP2C domain containing) (Ppm1k), nuclear gene encoding mitochondrial protein |
CNY 5,230.00 |
|
| MR217753L4 | Lenti ORF clone of Ppm1k (mGFP-tagged) - Mouse protein phosphatase 1K (PP2C domain containing) (Ppm1k), nuclear gene encoding mitochondrial protein |
CNY 5,230.00 |
