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Sh3pxd2a (NM_008018) Mouse Tagged ORF Clone
CAT＃: MG217612
- TrueORF®
Sh3pxd2a (tGFP-tagged) - Mouse SH3 and PX domains 2A (Sh3pxd2a) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_008018" in other vectors (4)
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CNY 12,350.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2310014D11Rik; AA589508; AI256723; AI413738; C230050L11; EG329070; Fish; Gm5098; Sh3md1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG217612 representing NM_008018
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCGCCTACTGCGTGCAAGATGCCACCGTGGTGGACGTGGAGAAGCGGAGGAGCCCCTCTAAACACT ATGTATACATTATCAACGTGACCTGGTCTGACTCCACCTCCCAGACTATCTACCGGAGGTACAGCAAGTT CTTCGACCTGCAGATGCAGCTTCTGGATAAGTTTCCTATTGAAGGTGGCCAGAAGGATCCGAAGCAAAGG ATTATTCCCTTTCTTCCAGGCAAGATCCTCTTCCGGAGAAGCCACATCCGCGACGTGGCTGTGAAGAGAC TAAAGCCCATCGATGAATACTGCAGGGCGCTTGTCCGGCTGCCGCCCCACATTTCACAGTGTGACGAAGT CTTCCGGTTCTTTGAGGCACGGCCTGAGGATGTCAACCCTCCAAAAGAAGACTATGGCAGTTCCAAGAGG AAATCAGTGTGGTTGTCCAGCTGGGCTGAGTCTCCCAAGAAGGACGTGACAGGTGCCGACACCAACGCCG AGCCCATGATCCTGGAACAGTACGTGGTGGTGTCCAACTATAAGAAACAAGAGAACTCGGAGCTGAGCCT CCAGGCCGGGGAGGTGGTAGATGTCATCGAGAAGAACGAAAGCGGCTGGTGGTTTGTGAGCACATCTGAA GAGCAAGGTTGGGTCCCCGCCACCTACTTGGAGGCCCAGAATGGCACACGAGACGACTCGGACATCAACA CCTCCAAGACTGGGGAAGTGTCCAAGAGACGCAAGGCACACCTGCGGCGCCTGGATCGCCGGTGGACCCT GGGCGGGATGGTCAACAGGCAGCACAGCCGAGAAGAGAAGTATGTCACTGTGCAGCCCTACACCAGCCAG AGCAAAGACGAGATCGGCTTCGAGAAGGGTGTCACCGTGGAGGTGATTCGAAAGAACCTGGAAGGCTGGT GGTACATCAGGTACCTCGGCAAAGAGGGTTGGGCACCAGCGTCCTACCTGAAGAAGGCCAAGGATGACCT GCCGACCCGGAAGAAGAACCTGGCGGGTCCGGTGGAGATCATAGGGAACATTATGGAGATCAGCAACCTT CTCAACAAGAAGGCATCTGGGGATAAGGAGGCTCCGGCTGAAGGCGAGGGATCCGAGGCCCCCATCACCA AGAAAGAGATCAGCTTACCGATCCTCTGCAACGCCTCCAATGGCAGCGCCTTGGCCATTCCCGAGAGGAC CACATCCAAGCTAGCCCAGGGCTCCCCAGCTGTGGCCAGGATCGCCCCTCAGAGGGCCCAGATCAGCTCC CCAAATCTGAGGACAAGACCTCCCCCGCGCAGAGAATCCAGCCTGGGGTTCCAGCTGCCAAAGCCGCCAG AGCCCCCTTCTGTTGAGGTAGAATACTACACCATTGCTGAATTCCAGTCCTGCATTTCTGACGGGATCAG CTTTCGAGGCGGACAGAAGGCAGAGGTCATCGACAAGAACTCGGGTGGTTGGTGGTACGTGCAGATCGGG GAGAAGGAGGGCTGGGCCCCAGCCTCATACATTGACAAGCGCAAGAAACCCAACCTCAGCCGCCGAACCA GCACTCTGACGCGGCCCAAGGTGCCGCCACCTGCGCCCCCCAGCAAGCCTAAGGAGGCCGAGGAGAATCC TGTGGGTGCCTGTGAGAGCCAGGGCTCCCCACTGAAGGTTAAATACGAGGAACCCGAGTATGACGTCCCT GCCTTTGGCTTTGACTCAGAGCCCGAGATGAATGAAGAGCCTTCAGGGGACAGAGGTTCAGGTGACAAGC ATCCCGCCCAGCCCCGAAGGATCTCGCCTGCCTCTTCCCTGCAACGGGCCCATTTCAAGGTGGGTGAGTC TTCTGAGGACGTGGCCCTGGAAGAGGAGACCATCTATGAGAATGAGGGCTTCAGGCCATACACAGAAGAC ACCCTGTCTGCCAGAGGCTCCTCTGGGGACAGTGACTCCCCTGGGAGCTCCTCTTTGTCCCTTGCCGTGA AAAACTCCCCTAAATCAGATTCCCCCAAATCCTCATCACTCCTAAAGCTCAAAGCAGAGAAGAATGCCCA GGCAGAACTGGGGAAAAACCAGTCCAACATCTCCTTCTCCTCCTCTGTCACCATCAGCACCACCTGTTCC TCCTCCTCATCCTCGTCCTCCTTGTCCAAGAACAATGGTGACCTGAAACCACGTTCTGCCTCAGATGCAG GTATCCGTGACACCCCTAAGGTTGGGACCAAGAAAGATCCTGATGTGAAGGCCGGGCTGGCCTCCTGCGC CCGAGCCAAGCCATCCGTGAGACCAAAGCCAGTCCTGAACCGAGCGGAGTCTCAAAGCCAGGAGAAGATG GATATTAGTTCCTTACGGCGCCAGCTGAGGCCCACAGGCCAGCTCCGGGGGGGCCTCAAGGGCTCTAGGA GTGAGGACTCAGAGCTGCCTCCACAGATGGCTTCTGAGGGATCCAGGCGAGGTTCTGCGGACATCATCCC TCTCACGGCCACCACTCCCCCGTGTGTCCCCAAAAAGGAATGGGAAGGGCAAGGCGCCACCTACGTGACG TGCAGCGCCTATCAGAAGGTCCAGGACTCGGAGATCAGCTTCCCCGAAGGCGCCGAGGTGCACGTGCTGG AGAAGGCGGAAAGTGGGTGGTGGTACGTGAGGTTTGGGGAGCTGGAGGGCTGGGCTCCTTCCCACTACTT GGTGGCCGAGGAGAACCAGCAACCTGACACAGCTAGCAAAGAGGGAGACACAGGAAAGAGCTCGCAGAAC GAGGGCAAGTCAGACAGCCTGGAAAAGATTGAGAAGCGTGTGCAGGCGCTCAACACTGTGAACCAGAGCA AGAGGGCCACCCCACCCATCCCCTCGAAGCCTCCCGGGGGCTTCGGCAAGACCTCGGGCACCGTAGCGGT GAAGATGAGGAACGGGGTCCGGCAAGTGGCCGTCAGGCCCCAATCTGTGTTTGTGTCTCCGCCACCCAAG GACAACAACCTGTCCTGTGCCCTTCGGAGGAACGAGTCGCTAACGGCCACCGACAGCCTCAGAGGTGTCC GCAGGAACTCCTCCTTTAGCACCGCACGGTCAGCAGCCGCTGAGGCCAAGGGCCGCCTGGCCGAGCGGGC TGCCAGCCAGGGCTCAGAATCGCCCCTGCTGCCTACCCAGCGCAAAGGCATCCCTGTCTCCCCCGTGCGT CCCAAGCCCATAGAGAAGTCCCAGTTTATCCACAACAACCTCAAGGATGTGTACATCTCGATTGCAGACT ATGAGGGGGACGAAGAGACGGCTGGCTTCCAGGAGGGGGTGTCCATGGAGGTGCTGGAGAAGAACCCCAA TGGCTGGTGGTACTGCCAGATCCTGGATGAGGTGAAGCCCTTCAAGGGCTGGGTACCCTCCAACTACCTT GAGAAGAAGAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217612 representing NM_008018
Red=Cloning site Green=Tags(s) MLAYCVQDATVVDVEKRRSPSKHYVYIINVTWSDSTSQTIYRRYSKFFDLQMQLLDKFPIEGGQKDPKQR IIPFLPGKILFRRSHIRDVAVKRLKPIDEYCRALVRLPPHISQCDEVFRFFEARPEDVNPPKEDYGSSKR KSVWLSSWAESPKKDVTGADTNAEPMILEQYVVVSNYKKQENSELSLQAGEVVDVIEKNESGWWFVSTSE EQGWVPATYLEAQNGTRDDSDINTSKTGEVSKRRKAHLRRLDRRWTLGGMVNRQHSREEKYVTVQPYTSQ SKDEIGFEKGVTVEVIRKNLEGWWYIRYLGKEGWAPASYLKKAKDDLPTRKKNLAGPVEIIGNIMEISNL LNKKASGDKEAPAEGEGSEAPITKKEISLPILCNASNGSALAIPERTTSKLAQGSPAVARIAPQRAQISS PNLRTRPPPRRESSLGFQLPKPPEPPSVEVEYYTIAEFQSCISDGISFRGGQKAEVIDKNSGGWWYVQIG EKEGWAPASYIDKRKKPNLSRRTSTLTRPKVPPPAPPSKPKEAEENPVGACESQGSPLKVKYEEPEYDVP AFGFDSEPEMNEEPSGDRGSGDKHPAQPRRISPASSLQRAHFKVGESSEDVALEEETIYENEGFRPYTED TLSARGSSGDSDSPGSSSLSLAVKNSPKSDSPKSSSLLKLKAEKNAQAELGKNQSNISFSSSVTISTTCS SSSSSSSLSKNNGDLKPRSASDAGIRDTPKVGTKKDPDVKAGLASCARAKPSVRPKPVLNRAESQSQEKM DISSLRRQLRPTGQLRGGLKGSRSEDSELPPQMASEGSRRGSADIIPLTATTPPCVPKKEWEGQGATYVT CSAYQKVQDSEISFPEGAEVHVLEKAESGWWYVRFGELEGWAPSHYLVAEENQQPDTASKEGDTGKSSQN EGKSDSLEKIEKRVQALNTVNQSKRATPPIPSKPPGGFGKTSGTVAVKMRNGVRQVAVRPQSVFVSPPPK DNNLSCALRRNESLTATDSLRGVRRNSSFSTARSAAAEAKGRLAERAASQGSESPLLPTQRKGIPVSPVR PKPIEKSQFIHNNLKDVYISIADYEGDEETAGFQEGVSMEVLEKNPNGWWYCQILDEVKPFKGWVPSNYL EKKN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008018 |
| ORF Size | 3372 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008018.4, NP_032044.2 |
| RefSeq Size | 10464 bp |
| RefSeq ORF | 3375 bp |
| Locus ID | 14218 |
| UniProt ID | O89032 |
| Gene Summary | Adapter protein involved in invadopodia and podosome formation, extracellular matrix degradation and invasiveness of some cancer cells. Binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1- or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. In association with ADAM12, mediates the neurotoxic effect of amyloid-beta peptide (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223641 | Sh3pxd2a (untagged) - Mouse SH3 and PX domains 2A (Sh3pxd2a), transcript variant 1, (10ug) |
CNY 14,350.00 |
|
| MR217612 | Sh3pxd2a (Myc-DDK-tagged) - Mouse SH3 and PX domains 2A (Sh3pxd2a), transcript variant 1 |
CNY 11,210.00 |
|
| MR217612L3 | Lenti ORF clone of Sh3pxd2a (Myc-DDK-tagged) - Mouse SH3 and PX domains 2A (Sh3pxd2a), transcript variant 1 |
CNY 13,110.00 |
|
| MR217612L4 | Lenti ORF clone of Sh3pxd2a (mGFP-tagged) - Mouse SH3 and PX domains 2A (Sh3pxd2a), transcript variant 1 |
CNY 13,110.00 |
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